| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | AM260480 | 1930349 = | 40 (1.360) | 8 (0.310) | 8/186 | 1.2 | 19.3% | coding (2771/2817 nt) | fdwA | Tungsten‑containing formate dehydrogenase alpha subunit |
| ? | AM260480 | 1930363 = | 32 (1.250) | coding (2757/2817 nt) | fdwA | Tungsten‑containing formate dehydrogenase alpha subunit | |||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
ATTTCCCGAAACTCCGGATAATCACCCAACAAAAAGGGCCGCCTGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATCGCCGCCTCGTAATAGCAGAACGGCACGAACACCGCCC > AM260480/1930216‑1930481 | atTTCCCGAAACTCCGGATAATCACCCAACAAAAAGGGCCGCCTGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCcg < 1:529050/134‑1 (MQ=255)atTTCCCGAAACTCCGGATAATCACCCAACAAAAAGGGCCGCCTGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCcg > 1:664548/1‑134 (MQ=255) caaAAAGGGCCGCCTGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAcc < 2:401772/134‑1 (MQ=255) gccgccTGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTa < 1:270956/134‑1 (MQ=255) ccgccTGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTa < 1:252924/133‑1 (MQ=255) ccgccTGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTa > 2:252924/1‑133 (MQ=255) ttacgaggcggcgatcaacaagctgaccaatgcggcactggaccccttcggcaagatcccggagttcaagtactgcgcggtgaagatggcactgGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACt > 1:1057738‑M1/95‑134 (MQ=255) cgccTGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACt > 1:443241/1‑134 (MQ=255) tGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAc > 2:412953/1‑119 (MQ=255) tGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAc < 1:412953/119‑1 (MQ=255) ggcgatcaacaagctgaccaatgcggcactggaccccttcggcaagatcccggagttcaagtactgcgcggtgaagatggcactgGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCg > 1:1077477‑M1/86‑134 (MQ=255) ggcgatcaacaagctgaccaatgcggcactggaccccttcggcaagatcccggagttcaagtactgcgcggtgaagatggcactgGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCg < 2:1077477‑M1/49‑1 (MQ=255) tcaacaagctgaccaatgcggcactggaccccttcggcaagatcccggagttcaagtactgcgcggtgaagatggcactgGGCGGCGCGCCGCCCAGTGCCATCTTc < 1:970582‑M1/27‑1 (MQ=255) aCGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGcc > 1:25661/1‑133 (MQ=255) aCGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGcc < 2:25661/133‑1 (MQ=255) ccccGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAgg < 2:484767/134‑1 (MQ=255) ccAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGTGTcc < 2:957089/134‑1 (MQ=255) ggaccccttcggcaagatcccggagttcaagtactgcgcggtgaagatggcactgGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCAt < 2:555541‑M1/79‑1 (MQ=255) tCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCgccgcc > 2:632061/1‑66 (MQ=255) tCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCgccgcc < 1:632061/66‑1 (MQ=255) tCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTgg > 1:61728/1‑134 (MQ=255) ccttcggcaagatcccggagttcaagtactgcgcggtgaagatggcactgGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGt < 2:633736‑M1/83‑1 (MQ=255) cgccAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATcg < 2:125172/134‑1 (MQ=255) ccAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAc > 2:615750/1‑64 (MQ=255) ccAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAc < 1:615750/64‑1 (MQ=255) cAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCcggcgg > 2:396910/1‑39 (MQ=255) cAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCcggcgg < 1:396910/39‑1 (MQ=255) cAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGgcgcgc < 2:707440/44‑1 (MQ=255) cAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGgcgcgc > 1:707440/1‑44 (MQ=255) cAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAcc > 1:397543/1‑64 (MQ=255) cAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAcc < 2:397543/64‑1 (MQ=255) ggATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATCgccgcc < 2:61728/134‑1 (MQ=255) ggATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATCgccgcc < 2:443241/134‑1 (MQ=255) tactgcgcggtgaagatggcactgGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATCGCCGCCTCGTaa < 2:146492‑M1/108‑1 (MQ=255) gccgccgTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATcg < 2:1079362/119‑1 (MQ=255) gccgccgTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATcg > 1:1079362/1‑119 (MQ=255) cgTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGcc < 1:64395/76‑1 (MQ=255) cgTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGcc > 2:64395/1‑76 (MQ=255) gTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAcc < 2:450967/46‑1 (MQ=255) gTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAcc > 1:1033929/1‑46 (MQ=255) gTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAcc < 2:1033929/46‑1 (MQ=255) gTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCAcc > 1:450967/1‑46 (MQ=255) gTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATCGCCGCCTCGTAATAGCAGAACg > 1:525932/1‑134 (MQ=255) gaagatggcactgGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGa < 2:970582‑M1/94‑1 (MQ=255) cTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATCGCCGCCTCGTAATAGCAGAACGGCACGAACACCg > 2:833032/1‑134 (MQ=255) cggcggCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCtt > 1:930918/1‑57 (MQ=255) cggcggCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCtt < 2:930918/57‑1 (MQ=255) cggcggCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATCGCCGCCTCGTAATAGCAGAACGGCACGAACACCGccc < 2:525932/134‑1 (MQ=255) | ATTTCCCGAAACTCCGGATAATCACCCAACAAAAAGGGCCGCCTGCGCTACCCGCGCAGACGGCCCCGCCAGGGCTTGCCGTCGGCCCTACACCGCCGCCAGGATCTGCCCGCCGCCGTAGCTCGACTGCTCCGGCGGCGCGCCGCCCAGTGCCATCTTCACCGCGCAGTACTTGAACTCCGGGATCTTGCCGAAGGGGTCCAGTGCCGCATTGGTCAGCTTGTTGATCGCCGCCTCGTAATAGCAGAACGGCACGAACACCGCCC > AM260480/1930216‑1930481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |