breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 193 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AssemblyC74_contig_1259,1940CT74.9% 2766.8 / inf 1718T48T (ACG→ACA) ‡rasttk_feature_annotation_tool=annotate_proteins_km er_v2Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
*AssemblyC74_contig_1259,1950GA74.6% 2717.1 / inf 1726T48M (ACG→ATG) ‡rasttk_feature_annotation_tool=annotate_proteins_km er_v2Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
*AssemblyC74_contig_1259,0620TC74.2% 3188.7 / inf 1676G92G (GGA→GGGrasttk_feature_annotation_tool=annotate_proteins_km er_v2Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
*AssemblyC74_contig_1533,1610TG73.8% 4748.8 / inf 2240T194P (ACC→CCC) rasttk_feature_annotation_tool=annotate_proteins_km er_v2Mobile element protein
*AssemblyC74_contig_11170CT73.5% 1402.8 / inf 1386intergenic (–/‑475)–/rasttk_feature_annotation_tool=annotate_proteins_km er_v2–/Two‑component transcriptional response regulator, OmpR family
*AssemblyC74_contig_431,2170AG73.4% 3528.0 / inf 1896intergenic (‑2/–)rasttk_feature_annotation_tool=annotate_proteins_km er_v2/–hypothetical protein/–
*AssemblyC74_contig_1259,2250AG72.5% 1456.2 / inf 993V38A (GTG→GCG) ‡rasttk_feature_annotation_tool=annotate_proteins_km er_v2Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
*AssemblyC74_contig_1259,2240CT72.5% 1358.2 / inf 993V38V (GTG→GTA) ‡rasttk_feature_annotation_tool=annotate_proteins_km er_v2Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
*AssemblyC74_contig_1533,1090TG71.5% 3951.8 / inf 2023K211T (AAA→ACA) rasttk_feature_annotation_tool=annotate_proteins_km er_v2Mobile element protein
*AssemblyC74_contig_11980AG70.2% 1201.8 / inf 885intergenic (–/‑394)–/rasttk_feature_annotation_tool=annotate_proteins_km er_v2–/Two‑component transcriptional response regulator, OmpR family
*AssemblyC74_contig_431,0460TG69.1% 3196.3 / inf 1892E57A (GAA→GCA) rasttk_feature_annotation_tool=annotate_proteins_km er_v2hypothetical protein
*AssemblyC74_contig_1650TC68.9% 288.5 / inf 805intergenic (–/‑527)–/rasttk_feature_annotation_tool=annotate_proteins_km er_v2–/Two‑component transcriptional response regulator, OmpR family
*AssemblyC74_contig_1660AG68.7% 282.2 / inf 809intergenic (–/‑526)–/rasttk_feature_annotation_tool=annotate_proteins_km er_v2–/Two‑component transcriptional response regulator, OmpR family
*AssemblyC74_contig_1259,2390GA67.8% 1140.3 / inf 1036G33G (GGC→GGTrasttk_feature_annotation_tool=annotate_proteins_km er_v2Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
*AssemblyC74_contig_301530AG66.6% 1130.1 / inf 1187T51T (ACA→ACGrasttk_feature_annotation_tool=annotate_proteins_si milarityMobile element protein
*AssemblyC74_contig_301660TC65.3% 1288.0 / inf 1459L56L (TTA→CTA) rasttk_feature_annotation_tool=annotate_proteins_si milarityMobile element protein
*AssemblyC74_contig_302100TC64.6% 1155.7 / inf 1449R70R (CGT→CGCrasttk_feature_annotation_tool=annotate_proteins_si milarityMobile element protein
*AssemblyC74_contig_474850GA64.3% 1642.0 / inf 1645D162N (GAT→AAT) rasttk_feature_annotation_tool=elepa@bvbrchypothetical protein
*AssemblyC74_contig_1533,6630CA64.1% 864.1 / inf 861T26T (ACG→ACTrasttk_feature_annotation_tool=annotate_proteins_km er_v2Mobile element protein
*AssemblyC74_contig_446660TC61.6% 2824.7 / inf 2975K157K (AAA→AAGrasttk_feature_annotation_tool=annotate_proteins_km er_v2hypothetical protein

Marginal new junction evidence (lowest skew 10 of 40 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AssemblyC74_contig_47 = 622NA (NA)22 (0.030)
+GATACGTCAAATGTTACGAA
10/208 0.9 5.7% noncoding (21/29 nt) direct CRISPR repeat with sequence agtgtagatgtaagtaattggaatacgtc
?AssemblyC74_contig_61 = 87 434 (0.300)coding (336/420 nt) rasttk_feature_annotation_tool=elepa@bvbrc hypothetical protein
* ? AssemblyC74_contig_47 487 =NA (NA)6 (0.010)
+CTTGTAACATTT
6/224 1.8 11.5% coding (486/879 nt) rasttk_feature_annotation_tool=elepa@bvbrc hypothetical protein
?AssemblyC74_contig_61 22 = 51 (0.040)coding (401/420 nt) rasttk_feature_annotation_tool=elepa@bvbrc hypothetical protein
* ? AssemblyC74_contig_61 1 =0 (0.000)4 (0.000) 3/230 3.0 2.1% intergenic (–/+2) –/rasttk_feature_annotation_tool=elepa@bvbrc –/hypothetical protein
?AssemblyC74_contig_61 = 78 380 (0.290)coding (345/420 nt) rasttk_feature_annotation_tool=elepa@bvbrc hypothetical protein
* ? AssemblyC74_contig_40 = 62NA (NA)230 (0.680)
+G
47/246 3.0 100% intergenic (–/+119) –/rasttk_feature_annotation_tool=annotate_proteins_km er_v2 –/Cold shock protein of CSP family
?AssemblyC74_contig_5 1 = 0 (0.000)intergenic (–/+540) –/rasttk_feature_annotation_tool=annotate_proteins_si milarity –/FIG00627359: hypothetical protein
* ? AssemblyC74_contig_13 1 =0 (0.000)185 (0.590) 43/248 3.2 73.7% coding (1/234 nt) rasttk_feature_annotation_tool=annotate_proteins_si milarity Transcriptional antiterminator of lichenan operon, BglG family
?AssemblyC74_contig_34 2046 = 132 (0.420)intergenic (‑223/–) rasttk_feature_annotation_tool=annotate_proteins_km er_v2/– PaaD‑like protein (DUF59) involved in Fe‑S cluster assembly/–
* ? AssemblyC74_contig_1 = 261377 (0.900)294 (0.700) 57/246 3.3 61.1% intergenic (–/‑331) –/rasttk_feature_annotation_tool=annotate_proteins_km er_v2 –/Two‑component transcriptional response regulator, OmpR family
?AssemblyC74_contig_7 2 = 0 (0.000)intergenic (–/+269) –/rasttk_feature_annotation_tool=annotate_proteins_km er_v2 –/Lysyl‑tRNA synthetase (class II) (EC 6.1.1.6)
* ? AssemblyC74_contig_25 = 85550 (0.000)218 (0.710)
+A
41/246 3.5 76.9% intergenic (+310/–) rasttk_feature_annotation_tool=annotate_proteins_km er_v2/– hypothetical protein/–
?AssemblyC74_contig_34 2046 = 132 (0.420)intergenic (‑223/–) rasttk_feature_annotation_tool=annotate_proteins_km er_v2/– PaaD‑like protein (DUF59) involved in Fe‑S cluster assembly/–
* ? AssemblyC74_contig_16 35042 =NA (NA)136 (0.450)
+G
42/246 4.1 100% intergenic (+88/–) rasttk_feature_annotation_tool=annotate_proteins_km er_v2/– hypothetical protein/–
?AssemblyC74_contig_4 = 189292 0 (0.000)intergenic (+1/–) rasttk_feature_annotation_tool=elepa@bvbrc/– hypothetical protein/–
* ? AssemblyC74_contig_12 = 59001277 (0.670)347 (0.850) 51/242 4.3 71.5% coding (337/672 nt) rasttk_feature_annotation_tool=annotate_proteins_km er_v2 Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
?AssemblyC74_contig_18 = 27071 6 (0.010)coding (2229/2232 nt) rasttk_feature_annotation_tool=annotate_proteins_km er_v2 hypothetical protein
* ? AssemblyC74_contig_41 = 246586 (0.580)475 (0.470) 81/248 4.7 45.0% intergenic (‑7/+105) rasttk_feature_annotation_tool=elepa@bvbrc/rasttk_feature_annotation_tool=elepa@bvbrc hypothetical protein/hypothetical protein
?AssemblyC74_contig_41 250 = 576 (0.570)intergenic (‑11/+101) rasttk_feature_annotation_tool=elepa@bvbrc/rasttk_feature_annotation_tool=elepa@bvbrc hypothetical protein/hypothetical protein