New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 2135599	61 (0.710)	4 (0.050)	4/328	NT	6.9%	coding (1152/2448 nt)	fadE	medium‑long chain acyl‑CoA dehydrogenase
	?	AE015451	= 2135585	52 (0.660)					coding (1166/2448 nt)	fadE	medium‑long chain acyl‑CoA dehydrogenase

CGCGGCCACGCTCGGTCAGGTGGTACTTGAGGATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/2135361‑2135599 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggcaccggtgccgCCAAGTACACCAGCCTGGTGACCGGCCAGTACGCCAATATCCGCGAGCAGTTCAACGTGCCATTGGCGGCCTTCGAAGGCATCCAGGAATCGCTGGCACGCATTGGCGGCAACGCCTGGCTGATGGACAGTGCCCGCCTGCTGACCGCCAAGGCCGTGGACCTGGGCGAAAAGCCCTCGGTGCTGTCGGCGATCCTCAA  <  AE015451/2135585‑2135387                                                                                                                                                                                                                                                                                                                                                                                                                                                        CGCGGCCACGCTCGGTCAGGTGGTACTTGAGGATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCG                                                                                                                                                                                                               >  2:845729/1‑233  GCGGCCACGCTCGGTCAGGTGGTACTTGAGGATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGC                                                                                                                                                                                                           >  1:666147/1‑236                                              CCGAGGGCTTTTCGCCCAGGTCCCCGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCcttcg                                                                                                                                                                                                              <  1:149558/189‑6                                                                               CGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCG                                                                                                                                                                                                                     >  1:526613/1‑149                                                                                GGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCG                                                                                                                                                                                                               >  2:657506/1‑154                                                                                                                                    AGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCG                                                                                                                                                                                                         <  2:759800/108‑1                                                                                                                                                                            TGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGCCAAGTACACCAGCCTGGTGACCGGCCAGTACGCCAATATCCGCGAGCAGTTCAACGTGCCATTGGCGGCCTTCGAAGGCATCCAGGAATCGCT                                                                                                           >  2:572535/1‑162                                                                                                                                                                                                   TACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGCCAAGTACACCAGCCTGGGGACCGG                                                                                                                                                                                >  2:1489846/1‑70                                                                                                                                                                                                           GGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGCCAAGTACACCAGCCTGGTGACCGGCCAGTACGCCAATATCCGCCAGCAGTTCAACGTGCCATTGGCGGCCTTC                                                                                                                               >  1:184193/1‑111                                                                                                                                                                                                                       GGTGTACTTGGCGGCACCGGTGCCGCCAAGTACACCAGCCTGGTGACCGGCCAGTACGCCAATATCCGCGAGCAGTTCAACGTGCCATTGGCGGCCTTCGAAGGCATCCAGGAATCGCTGGCACGCATTGGCGGCAACGCCTGGCTGATGGACAGTTCCCGCCTGCTGACCGCCAAGGCCGTGGgc                                        >  1:157882/1‑184                                                                                                                                                                                                                                              CGCCAAGTACACCAGCCTGGTGACCGGCCAGTACGCCAATATCCGCGAGCAGTTCAACGTGCCATTGGCGGCCTTCGAAGGCATCCAGGAATCGCTGGCACGCATTGGCGGCAACGCCTGGCTGATGGACAGTGCCCGCCTGCTGACCGCCAAGGCCGTGGACCTGGGCGAAAAGCCCTCGGGGCTGTCGGCGCTCCTCAA  >  1:143921/1‑201                                                                                                                                                                                                                                              CGCCAAGTACACCAGCCTGGTGACCGGCCAGTACGCCAATATCCGCGAGCAGTTCAACGTGCCATTGGCGGCCTTCGAAGGCATCCAGGAATCGCTGGCACGCATTGGCGGCAACGCCTGGCTGATGGACAGTGCCCGCCTGCTGACCG                                                      >  2:526611/1‑149                                                                                                                                                                                                                                                                                                                                                                                                                                                        CGCGGCCACGCTCGGTCAGGTGGTACTTGAGGATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/2135361‑2135599 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggcaccggtgccgCCAAGTACACCAGCCTGGTGACCGGCCAGTACGCCAATATCCGCGAGCAGTTCAACGTGCCATTGGCGGCCTTCGAAGGCATCCAGGAATCGCTGGCACGCATTGGCGGCAACGCCTGGCTGATGGACAGTGCCCGCCTGCTGACCGCCAAGGCCGTGGACCTGGGCGAAAAGCCCTCGGTGCTGTCGGCGATCCTCAA  <  AE015451/2135585‑2135387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.