New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5644984	103 (1.200)	3 (0.040)	3/342	NT	2.8%	coding (337/2184 nt)	PP_4954	conserved membrane protein of unknown function
	?	AE015451	= 5645051	110 (1.330)					coding (404/2184 nt)	PP_4954	conserved membrane protein of unknown function
Rejected: Frequency below/above cutoff threshold.

CCAGAACGAGATGGCCCTGCTGATTCCGCTGTTCCTCGGCATCATCGCCAGCGCCCTGGCCGAAACCGACGACAGCTGGCAGGGCCGCCTCAGCGCCCTGGCCGTTACCCTGGTGTGCTTCGCCATCGCTGCACTGGCAGTCGAGTTGCTATTCCCCTACCCCTGGATCTTCGTCGTTGCCCTGGCCCTGGCTGCCTTTGGCCTGACCATGCTCGGGGCGCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5644761‑5644984 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgctggTCCACGCCGATCATGGTGTAGACCGCAGTGATCAAGGTGGCCGAAGCGATGGCGCCGTAGCGCTCGCCCAGCGCCCCGAGCATGGTCAGGCCAAAGGCAGCCAGGGCCAGGGCAACGACGAAGATCCAGGGGTAGGGGAATAGCAACTCGACTGCCAGTGCAGCGATGGCGAAGCACACCAGGGTAACGGCCAGGGCGCTGAGGCGGCCCTGCCAGCTGTCGTCGGTTTCG  <  AE015451/5645051‑5644821                                                                                                                                                                                                                                                                                                                                                                                                                                                                         CCAGAACGAGATGGCCCTGCTGATTCCGCTGTTCCTCGGCATCATCGCCAGCGCCCTGGCCGAAACCGACGACAGCTGGCAGGGCCGCCTCAGCGCCCTGGCCGTTACCCTGGTGTGCTTCGCCATCGCTGCACTGGCAGTCGAGTTGCTATTCCCCTACCCCTGGATCTTCGTCGTTGCCCTGGCCCTGGCTGCCTTTGGCCTGACCATGCTCGGGGCGCTGG                                                                                                                                                                                                                                         >  2:1160155/1‑224                             CTGTTCCTCGGCATCATCGCCAGCGCCCTGGCCGAAACCGACGACAGCTGGCAGGGCCGCCTCAGCGCCCTGGCCGTTACCCTGGTGTGCTTCGCCATCGCTGCACTGGCAGTCGAGTTGCTATTCCCCTACCCCTGGATCTTCGTCGTTGCCCTGGCCCTGGCTGCCTTTGGCCTGACCATGCTCGGGGCGCTG                                                                                                                                                                                                                                          <  2:566006/195‑1                                                                                                                                                                   CTGGATCTTCGTCGTTGCCCTGGCCCTGGCTGCCTTTGGCCTGACCATGCTCGGGGCGCTGGTCCACGCCGATCATGGTGTAGACCGCAGTGATCAAGGTGGCCGAAGCGATGGCGCCGTAGCGCTCGCCCGGCGCCCCGAGCATGGTCAGGCCAAAGGCAGCCAGc                                                                                                                                >  1:1289106/1‑166                                                                                                                                                                         CTTCGTCGTTGCCCTGGCCCTGGCTGCCTTTGGCCTGACCATGCTCGGGGCGCTGGTCCACGCCGATCATGGTGTAGACCGCAGGGATCAAGGTGGCCGAAGCGATGGCGCCGTAGCGCTCGCCCAGCGCCCCGAGCATGGT                                                                                                                                                   >  2:1047120/1‑142                                                                                                                                                                              TCGTTGCCCTGGCCCTGGCTGCCTTTGGCCTGACCATGCTCGGGGCGCTGGTCCACGCCGATCATGGTGTAGACCGCAGTGATCAAGGTGGCCGCCGCGATGGCGCCGTAGCGCTCGCCCAGCGCCCCGAGCATG                                                                                                                                                     >  2:131532/1‑135                                                                                                                                                                                                                            GCTGGTCCACGCCGATCATGGTGTAGACCGCAGTGATCAAGGTGGCCGAAGCGATGGCGCCGTAGCGCTCGCCCAGCGCCCCGAGCATGGTCAGGCCAAAGGCAGCCAGGGCCAGGGCAACGACGAAGATCCAGGGGTAG                                                                                                  >  2:1189855/1‑140                                                                                                                                                                                                                             gTGGTCCACGCCGATCATGGTGTAGACCGCAGTGATCAAGGTGGCCGAAGCGATGGCGCCGTAGCGCTCGCCCAGCGCCCCGAGCATGGTCAGGCCAAAGGCAGCCAGGGCCAGGGCAACGACGAAGATCCAGGGGTAGGGGAATAGCAACTCGACTGCCAGTGCAGCGATGGCGAAGCACACCAGGGTAACGGCCAGGGCGCTGAGGCGGCCCTGCCAGCTGTCGTCGGTTTCG  <  2:789958/234‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                         CCAGAACGAGATGGCCCTGCTGATTCCGCTGTTCCTCGGCATCATCGCCAGCGCCCTGGCCGAAACCGACGACAGCTGGCAGGGCCGCCTCAGCGCCCTGGCCGTTACCCTGGTGTGCTTCGCCATCGCTGCACTGGCAGTCGAGTTGCTATTCCCCTACCCCTGGATCTTCGTCGTTGCCCTGGCCCTGGCTGCCTTTGGCCTGACCATGCTCGGGGCGCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5644761‑5644984 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgctggTCCACGCCGATCATGGTGTAGACCGCAGTGATCAAGGTGGCCGAAGCGATGGCGCCGTAGCGCTCGCCCAGCGCCCCGAGCATGGTCAGGCCAAAGGCAGCCAGGGCCAGGGCAACGACGAAGATCCAGGGGTAGGGGAATAGCAACTCGACTGCCAGTGCAGCGATGGCGAAGCACACCAGGGTAACGGCCAGGGCGCTGAGGCGGCCCTGCCAGCTGTCGTCGGTTTCG  <  AE015451/5645051‑5644821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.