New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5790883	84 (0.980)	3 (0.040)	3/340	NT	3.6%	coding (835/1065 nt)	hemE	Uroporphyrinogen decarboxylase
	?	AE015451	= 5790885	79 (0.960)					coding (833/1065 nt)	hemE	Uroporphyrinogen decarboxylase
Rejected: Frequency below/above cutoff threshold.

TGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAGTGCCGTGGCCATAGCTGGCCAGGATGCGCGCCACTTCCTTGCGAATGGCCTCGGGCTTGGCATACAGCACGGTCGGGTCCATGTTGCCTTGCAGCGCCACCTTGTCACCCACGCGGCGACG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5790644‑5790883 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggcgacgCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGGCCACGGCATCACGCCGGAAGTTGACCCGGAACATGCGGGTGCATTCATCAACGCCGTGCACGAACTGTCGGCGCAGTACCACCAGTGAC  <  AE015451/5790885‑5790652                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            TGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAGTGCCGTGGCCATAGCTGGCCAGGATGCGCGCCACTTCCTTGCGAATGGCCTCGGGCTTGGCATACAGCACGGTCGGGTCCATGTTGCCTTGCAGCGCCACCCTGTCACCCACGCGGC                                                                                                                                                                                                                                                >  1:462069/1‑236   CATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAGTGCCGTGGCCATAGCTGGCCAGGATGCGCGCCACTTCCTTGCGAATGGCCTCGGGCTTGGCATACAGCACGGTCGGGTCCATGTTGCCTTGCAGCGCCACCTTGTCACCCACGCGGCG                                                                                                                                                                                                                                               >  1:888845/1‑235                                                                                                                                                                                                TCGGGTCCATGTTGCCTTGCAGCGCCACCTTGTCACCCACGCGGCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGG                                                                                                                         <  1:439784/164‑1                                                                                                                                                                                                TCGGGTCCATGTTGCCTTGCAGCGCCACCTTGTCACCCACGCGGCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCAT                                                                                                                                                             >  2:841636/1‑128                                                                                                                                                                                                             GCCTTGCAGCGCCACCTTGTCACCCACGCGGCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGCCCGTGCTGTATGCCAAGCCCGAGGC                                                                                                                                                                >  2:439782/1‑112                                                                                                                                                                                                                                         CGGCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAG                                                                                                                                                                  <  1:1075479/82‑1                                                                                                                                                                                                                                         CGGCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAG                                                                                                                                                                  >  2:1075476/1‑82                                                                                                                                                                                                                                          GGCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGGCCACGGCATCACGCCGGAAGTTGACCCGGAACATGCGGGTGCATTCATCAACGCCGTGCACGAACTGTCGGCGCAGTACCACCA       <  1:1155769/236‑1                                                                                                                                                                                                                                          GGCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGGCCACGGCATCACGCCGGAAGTTGACCCGGAACATGCGGGTGCATTCATCAACGCCGTGCACGAACTGTCGGCGCAGTACCACCA       <  1:300122/236‑1                                                                                                                                                                                                                                          GGCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGGCCACGGCATCACGCCGGAAGTTGACCCGGAACATGCGGGTGCATTCATCAACGCCGTGCACGAACTGT                       <  2:922728/220‑1                                                                                                                                                                                                                                          GGCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGG                                                                                           <  2:1337865/152‑1                                                                                                                                                                                                                                          GGCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGG                                                                                           >  1:1337868/1‑152                                                                                                                                                                                                                                          GGCGACGCCCGTCGCCGCGTGGGTGACAAGGTCGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGGCCACGGCATCACGCCGGAAGTTGACCCGGAACATGCGGGTGCATTCATCAACGCCGTGCA                               <  1:418019/212‑1                                                                                                                                                                                                                                           GCGACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGG                                                                                                             <  2:1228648/133‑1                                                                                                                                                                                                                                              ACGCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGGCCACGGCATCACGCCGGAAGTTGACCCGGAACATGCGGGTGCATTCATCAACGCCGTGCACGAACTGTCGGCGCAG               <  2:632222/224‑1                                                                                                                                                                                                                                                GCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGGCCACGGCATCACGCCGGAAGTTGACCCGGAACATGCGGGTGCATTCATCAACGCCGTGCACGAACTGTCGGCGCAGTACCACCAGTGAC  <  1:767941/235‑1                                                                                                                                                                                                                                                GCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGGCCACGGCATCACGCCGGAAGTTGACCCGGAACATGCGGGTGCATTCATCAACGCCGTGCACGAACTGTCGGCGCAGTACCACCAGTGAC  <  1:577022/235‑1                                                                                                                                                                                                                                                GCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGGCCACGGCATCACGCCGGAAGTTGACCCGGAACATGCGGGTGCATTCATCAACGCCGTGCACGAACTGTCGGCGCAGTACCACCAGTGAC  <  1:1305647/235‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            TGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAGTGCCGTGGCCATAGCTGGCCAGGATGCGCGCCACTTCCTTGCGAATGGCCTCGGGCTTGGCATACAGCACGGTCGGGTCCATGTTGCCTTGCAGCGCCACCTTGTCACCCACGCGGCGACG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5790644‑5790883 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggcgacgCCCGTCGCCGCGTGGGTGACAAGGTGGCGCTGCAAGGCAACATGGACCCGACCGTGCTGTATGCCAAGCCCGAGGCCATTCGCAAGGAAGTGGCGCGCATCCTGGCCAGCTATGGCCACGGCACTGGCCATGTGTTCAACCTTGGCCACGGCATCACGCCGGAAGTTGACCCGGAACATGCGGGTGCATTCATCAACGCCGTGCACGAACTGTCGGCGCAGTACCACCAGTGAC  <  AE015451/5790885‑5790652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.