New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 3643473	71 (0.830)	4 (0.050)	4/336	NT	5.6%	coding (574/804 nt)	PP_3210	ABC transporter, permease protein
	?	AE015451	= 3643535	67 (0.830)					coding (512/804 nt)	PP_3210	ABC transporter, permease protein

AGGCTGAACAGCGCCACGCCAGTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTGCAGCAGGATCGCATAGGGAAACAGACTGGCCTCCA  >  AE015451/3643301‑3643757                                                                                                                                                                                                                                           |                                                                                                                                                                                                                               aGGCGGAACAGCGCCACGCCAGTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACtt                                                                                                                                                                                                                                <  1:1211279/235‑1 (MQ=255)            cgccacgccaGTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTg                                                                                                                                                                                                                     <  1:1469266/235‑1 (MQ=255)            cgccacgccaGTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTg                                                                                                                                                                                                                     <  1:1189585/235‑1 (MQ=255)                acgccaGTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGgcgc                                                                                                                                                                                                                 <  1:640818/235‑1 (MQ=255)                 cgccaGTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGcgcg                                                                                                                                                                                                                >  1:1508319/1‑235 (MQ=255)                              gATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGGTCAGATGGAACa                                                                                                                                                                                                  >  1:125142/1‑236 (MQ=255)                                  aaCACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGtt                                                                                                                                                                                              <  1:1490395/236‑1 (MQ=255)                                   acacCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGGTCCGATGGAACAGGtt                                                                                                                                                                                              >  1:221499/1‑235 (MQ=255)                                       cAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATCCGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTgccagc                                                                                                                                                                                                                          >  1:232521‑M2/1‑197 (MQ=255)                                            cggcgAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGCTGGAACAGGTTGAg                                                                                                                                                                                           >  2:726153/1‑229 (MQ=255)                                             ggcgAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGa                                                                                                                                                                                                     >  1:1437250/1‑218 (MQ=255)                                                 aaTAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGt                                                                                                                                                                                               >  2:870135/1‑220 (MQ=255)                                                   tAGGCGGGGGATGTTGAGCTGTAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTg                                                                                                                                                                                                                     <  1:103754/196‑1 (MQ=255)                                                    aGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCCCCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGgcgc                                                                                                                                                                                                                 <  1:197570/199‑1 (MQ=255)                                                       cGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCt                                                                                                                                                                         <  1:1358094/236‑1 (MQ=255)                                                         ggggATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTg                                                                                                                                                                        <  1:1001721/235‑1 (MQ=255)                                                          gggATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTgccagcggcttggcctt                                                                                                                                                                                                               >  2:707965‑M2/1‑178 (MQ=255)                                                                   aGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGtt                                                                                                                                                                                              <  1:532141/203‑1 (MQ=255)                                                                    gCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGgcgc                                                                                                                                                                                                                 <  1:1317911/183‑1 (MQ=255)                                                                     gtGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTg                                                                                                                                                                                                                     <  2:72060/177‑1 (MQ=255)                                                                     cTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGa                                                                                                                                                                             <  1:1234619/218‑1 (MQ=255)                                                                             ccGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGACCTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGtt                                                                                                                                                                                                             <  1:247716/178‑1 (MQ=255)                                                                                           tGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAg                                                                                                                                                             <  2:538877/212‑1 (MQ=255)                                                                                           tGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGaa                                                                                                                                     <  1:1075161/236‑1 (MQ=255)                                                                                                cGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTg                                                                                                                                                                        <  1:445150/196‑1 (MQ=255)                                                                                                 gCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACg                                                                                                                                                         <  1:735671/210‑1 (MQ=255)                                                                                                 gCCAGGCCCGCACCCGGGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCg                                                                                                                                         <  1:611043/226‑1 (MQ=255)                                                                                                    aGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCg                                                                                                                                                <  1:995892/216‑1 (MQ=255)                                                                                                      gCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTgc                                                                                                                                                                                                                              <  2:1118438‑M2/136‑3 (MQ=255)                                                                                                      gCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCggg                                                                                                                                                                                                              >  2:1266149/1‑151 (MQ=255)                                                                                                      gCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGccacca                                                                                                                          >  1:1107179/1‑236 (MQ=255)                                                                                                        ccGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGc                                                                                                                        >  1:1073100/1‑236 (MQ=255)                                                                                                          gCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAg                                                                                                                                                             <  1:1375110/197‑1 (MQ=255)                                                                                                          gCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAg                                                                                                                                                             >  2:1375107/1‑197 (MQ=255)                                                                                                           ctgggcctgcgcagcgtcaaccctggcctgctcaacctgttccatctgaaccgcgccAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTATGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGAGGGAACAgg                                                                                                                                                                                                <  2:1428409‑M1/104‑1 (MQ=255)                                                                                                           ctgggcctgcgcagcgtcaaccctggcctgctcaacctgttccatctgaaccgcgccAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCACCAGCACTTGCCAGCGGCTg                                                                                                                                                                                                                     <  1:71093‑M1/83‑1 (MQ=255)                                                                                                            tgggcctgcgcagcgtcaaccctggcctgctcaacctgttccatctgaaccgcgccAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCgg                                                                                                                                                                                                                        <  2:1425219‑M1/80‑1 (MQ=255)                                                                                                             cccGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTg                                                                                                                     <  1:611681/234‑1 (MQ=255)                                                                                                              ccGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCGAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCa                                                                                                                                                              <  1:717911/192‑1 (MQ=255)                                                                                                              ccGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCt                                                                                                                                                                         <  2:1341855/181‑1 (MQ=255)                                                                                                                 tGCCGCCCGTACCGGCCACGAACTCGGCCACCACCGCGCCGAGCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGGCAGCGGCTGGCGCGGTTCAGATGGAACAGGtt                                                                                                                                                                                              <  1:1183455/157‑1 (MQ=255)                                                                                                                      cagcgtcaaccctggcctgctcaacctgttccatctgaaccgcgccAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAAc                                                                                                                                                                                                   <  1:707723‑M1/101‑1 (MQ=255)                                                                                                                       ccGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATc                                                                                                            <  2:548756/233‑1 (MQ=255)                                                                                                                        cGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGAAGGCGCATCAGCACTTGCCATCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCa                                                                                                           >  2:1426655/1‑233 (MQ=255)                                                                                                                         gTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAACAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCCGa                                                                                                                                                                                                         >  2:85852/1‑138 (MQ=255)                                                                                                                            ccGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATc                                                                                                            <  1:870137/228‑1 (MQ=255)                                                                                                                                 ctggcctgctcaacctgttccatctgaaccgcgccAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGcggtgca                                                                                                                                                                                                           <  1:781381‑M1/93‑4 (MQ=255)                                                                                                                                  acgaACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGGGGTTCACATGGAACAGGTCGAGCAg                                                                                                                                                                                        <  2:525966/146‑1 (MQ=255)                                                                                                                                     taCTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAACGCGCTGGGAATGCGCGGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCATGGCGCAGATCACCAACGCCAAGGt                                                                                              <  2:928850/232‑1 (MQ=255)                                                                                                                                      aCTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCgtg                                                                                          <  1:1240849/236‑1 (MQ=255)                                                                                                                                       cTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGtt                                                                                                                                                                                              <  1:1160255/135‑1 (MQ=255)                                                                                                                                       cTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGtt                                                                                                                                                                                              >  2:1160252/1‑135 (MQ=255)                                                                                                                                        tCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGtt                                                                                                                                                                                              <  2:121563/134‑1 (MQ=255)                                                                                                                                            ccacccCCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCg                                                                                       <  2:676877/233‑1 (MQ=255)                                                                                                                                                caccGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCa                                                                                                                                                                                                           <  2:411162/113‑1 (MQ=255)                                                                                                                                                caccGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGc                                                                                   <  2:1107176/233‑1 (MQ=255)                                                                                                                                                acccGGGCCGAGCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGATg                                                                                                                                                                        <  2:479559/146‑1 (MQ=255)                                                                                                                                                     cgcCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAGTGCGCAGGCGCAACAGCACTTgccagcggcttggccttgatcggcgcggtggtggccgagttcgt                                                                                                                                                                                    >  2:565200‑M2/1‑87 (MQ=255)                                                                                                                                                        cGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCgatg                                                                                                                                             >  2:1103669/1‑167 (MQ=255)                                                                                                                                                        cGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATc                                                                                                                                 >  1:67491/1‑179 (MQ=255)                                                                                                                                                              aGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGcc                                                                                                   <  1:1258487/203‑1 (MQ=255)                                                                                                                                                                gCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGtt                                                                                                                                                                                              <  2:457956/110‑1 (MQ=255)                                                                                                                                                                  gaaGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCt                                                                                                                                                                         <  1:291068/128‑1 (MQ=255)                                                                                                                                                                   cAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGgcgc                                                             <  1:772954‑M1/235‑1 (MQ=255)                                                                                                                                                                          gGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTgg                                                                                                                                                  >  2:114210‑M1/2‑144 (MQ=255)                                                                                                                                                                            GCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGccac                                                    >  1:1209314‑M1/1‑236 (MQ=255)                                                                                                                                                                            GCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGccac                                                    >  1:948703‑M1/1‑236 (MQ=255)                                                                                                                                                                             CGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGc                                                                                      <  2:528009‑M1/201‑1 (MQ=255)                                                                                                                                                                              gATGCGCAGGCCAGCGAAAAAGGACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTgccagcggcttggccttgatcggcgcggtggtggccgagttcgtcgccggtacgggcggcacgggtgcgggcctggcgtatcagatcctgcaagcgggattccagctcaacatcccccgcct                                                                                                      <  1:44498‑M2/184‑123 (MQ=255)                                                                                                                                                                              gATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGGTGGAACAGGGTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGGTGGCGGTGATCGGGaa                                                                                                                                     >  2:233759/1‑153 (MQ=255)                                                                                                                                                                                tGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATc                                                                                                            <  2:1028653/176‑1 (MQ=255)                                                                                                                                                                                 gcgcAGGCCAGCGAAAAAGGACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTgccagcggcttggccttgatcggcgcggtggtggccgagttcgtcgccggtacgggcggcacgggtgcgggcctggcgtatcagatcctgcaagcgggattccagctcaacatcccccgcctatt                                                                                                   >  2:44498‑M2/1‑59 (MQ=255)                                                                                                                                                                                  ggcAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGccac                                                    <  1:993718/229‑1 (MQ=255)                                                                                                                                                                                         cAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCa                                                                                                                                                                                         <  1:480841/90‑1 (MQ=255)                                                                                                                                                                                         cAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCa                                                                                                                                                                                         >  2:480839/1‑90 (MQ=255)                                                                                                                                                                                         cAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTAc                                        >  1:975092/1‑235 (MQ=255)                                                                                                                                                                                          aGCGAAAAAGCACGGTAAGGCGCGGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAgatgatgat                                                                      <  2:363560/204‑1 (MQ=255)                                                                                                                                                                                           gCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTg                                     <  1:420555/236‑1 (MQ=255)                                                                                                                                                                                            cGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTg                                     <  1:305565/235‑1 (MQ=255)                                                                                                                                                                                             gAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGGTGAGCAGGCCAGGGGTGACGCTGCGCAGGCCCAg                                                                                                                                                             >  2:177444/1‑114 (MQ=255)                                                                                                                                                                                                    cACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCACCGCCAAg                                                                                                >  1:1264468/1‑168 (MQ=255)                                                                                                                                                                                                    cACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTGCAGCAGGa                             <  1:578057/235‑1 (MQ=255)                                                                                                                                                                                                         tAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAgcgg                                                                >  1:504137/1‑195 (MQ=255)                                                                                                                                                                                                           aGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGtt                                          <  1:836577/215‑1 (MQ=255)                                                                                                                                                                                                             gcgcTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTGCAGCAGGATCGCATAgg                    <  1:1256799/235‑1 (MQ=255)                                                                                                                                                                                                                    gAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGaa                                                                                                                                     <  2:811567/115‑1 (MQ=255)                                                                                                                                                                                                                    gAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGaa                                                                                                                                     >  1:811569/1‑115 (MQ=255)                                                                                                                                                                                                                    gAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGGTGGCGATGATCGGGAAGATCGCCACCAGCGCGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGcgcggc                                                          >  1:304747/1‑188 (MQ=255)                                                                                                                                                                                                                    gAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGATGAGCAGGCCAGGGTTGACGCTGCGCAGCCCCAGGACGGTGTTGGCga                                                                                                                                               >  2:441709/1‑105 (MQ=255)                                                                                                                                                                                                                              gcgcAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTGCAGCAGGATCGCATAGGGAAACAGACTGGCCTCCa  >  1:377513/1‑236 (MQ=255)                                                                                                                                                                                                                                gcAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGGTACCTGcagcag                               >  2:1189145/1‑205 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                               AGGCTGAACAGCGCCACGCCAGTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTGCAGCAGGATCGCATAGGGAAACAGACTGGCCTCCA  >  AE015451/3643301‑3643757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.