New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4591278	28 (0.640)	3 (0.070)	3/324	NT	11.0%	coding (349/816 nt)	liuC	methylglutaconyl‑CoA hydratase
	?	AE015451	= 4591409	22 (0.530)					coding (480/816 nt)	liuC	methylglutaconyl‑CoA hydratase

TGGCCATCGACCAGTTGGCCGAAGACGCCAGCCTGCGCTTTGTGCTGCTGCGCGGCCGTGGCCGGCACTTCAGCGCCGGTGCCGACCTGGCCTGGATGCAGCAGTCGGCGCAACTGGACTTCAACACCAACCTGGATGATGCCCACGAACTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/4591048‑4591278 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcggcgcgcGGCGCGTTCGCCAATGGCCTTGACCACGAACGGGCTGATGACCGCCGGGGCCAGGCCGATGCGTACTTCCGACAGGCACAGTTGGGCGTCTTCGGCGCCGATGGCCATGTCGCAGCAGCTGATCAGGCCCAGCGCGCCGCCAAAGGCTGCACCTTGCACCACGGCCAGGGTCGGGGCCTTGAGGCGATGCAGGGCGTACATCAGCTCGCCCAGTTCGTGGGCATCATCCAGGT  <  AE015451/4591409‑4591177                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  TGGCCATCGACCAGTTGGCCGAAGACGCCAGCCTGCGCTTTGTGCTGCTGCGCGGCCGTGGCCGGCACTTCAGCGCCGGTGCCGACCTGGCCTGGATGCAGCAGTCGGCGCAACTGGACTTCAACACCAACCTGGATGATGCCCACGAACTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCGGC                                                                                                                                                                                                                                        <  1:456508/234‑1                                            tCTGCTGCGCGGCCGTGGCCGGCACTTCAGCGCCGGTGCCGACCTGGCCTGGATGCAGCAGTCGGCGCAACTGGACTTCAACACCAACCTGGATGATGCCCACGAACTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCGGCGCGTTCGCCAATGGCCTTGACCACGAACGGGCTGATGACCGCCGG                                                                                                                                                                                           <  2:554149/235‑1                                                             GCCGGCACTTCAGCGCCGGTGCCGACCTGGCCTGGATGCAGCAGTCGGCGCAACTGGACTTCAACACCAACCTGGATGATGCCCACGAACTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCGGCGCGTTCGCCAATGGCCTTGACCACGAACGGGCTGATGACCGCCGGGGCCAGGCCGATGCGTA                                                                                                                                                                          <  1:426241/236‑1                                                                                                                                                    gACTGGGCGAGCGGATGGACGCCCTGCATCGACTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCG                                                                                                                                                                                                                                              <  2:56398/80‑1                                                                                                                                                                          CCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGG                                                                                                                                                                                                                                                   <  1:254303/54‑1                                                                                                                                                                          CCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGG                                                                                                                                                                                                                                                   >  2:254301/1‑54                                                                                                                                                                                                                         CCTTTGGCGGCGCGCGGCGCGTTCGCCAATGGCCTTGACCACGAACGGGCTGATGACCGCCGGGGCCAGGCCGATGCGTACTTCCGACAGGCACAGTTGGGCGTCTTCGGCGCC                                                                                                                                        >  1:36997/1‑114                                                                                                                                                                                                                                 GGCGCGCGGCGCGTTCGCCAATGGCCTTGACCACGAACGGGCTGATGACCGCCGGGGCCAGGCCGATGCGTACTTCCGACAGGCACAGTTGGGCGTCTTCGGCGCCGATGGCCATGTCGCAGCAGCTGATCAGGCCCAGCGCGCCGCCAAAGGCTGCACCTTGCACCACGGCCAGGGTCGGGGCCTTGAGGCGATGCAGGGCGTACATCAGCTCGCCCAGTTCGTGGGC             >  2:531177/1‑229                                                                                                                                                                                                                                  GCGCGCGGCGCGTTCGCCAATGGCCTTGACCACGAACGGGCTGATGACCGCCGGGGCCAGGCCGATGCGTACTTCCGACAGGCACAGTTGGGCGTCTTCGGCGCCGATGGCCATGTCGCAGCAGCTGATCAGGCCCAGCGCGCC                                                                                                 <  2:505696/144‑1                                                                                                                                                                                                                                      GCGGCGCGTTCGCCAATGGCCTTGACCACGAACGGGCTGATGACCGCCGGGGCCAGGCCGATGCGTACTTCCGACAGGCACAGTTGGGCGTCTTCGGCGCCGATGGCCATGTCGCAGCAGCTGATCAGGCCCAGCGCGCCGCCAAAGGCTGCACCTTGCACCACGGCCAGGGTCGGGGCCTTGAGGCGATGCAGGGCGTACATCAGCTCGCCCAGTTCGTGGGCATCATCCAGGT  <  1:356739/235‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  TGGCCATCGACCAGTTGGCCGAAGACGCCAGCCTGCGCTTTGTGCTGCTGCGCGGCCGTGGCCGGCACTTCAGCGCCGGTGCCGACCTGGCCTGGATGCAGCAGTCGGCGCAACTGGACTTCAACACCAACCTGGATGATGCCCACGAACTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/4591048‑4591278 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcggcgcgcGGCGCGTTCGCCAATGGCCTTGACCACGAACGGGCTGATGACCGCCGGGGCCAGGCCGATGCGTACTTCCGACAGGCACAGTTGGGCGTCTTCGGCGCCGATGGCCATGTCGCAGCAGCTGATCAGGCCCAGCGCGCCGCCAAAGGCTGCACCTTGCACCACGGCCAGGGTCGGGGCCTTGAGGCGATGCAGGGCGTACATCAGCTCGCCCAGTTCGTGGGCATCATCCAGGT  <  AE015451/4591409‑4591177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.