New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4591278	28 (0.640)	3 (0.070)	3/324	NT	11.0%	coding (349/816 nt)	liuC	methylglutaconyl‑CoA hydratase
	?	AE015451	= 4591409	22 (0.530)					coding (480/816 nt)	liuC	methylglutaconyl‑CoA hydratase

ACCTGGATGATGCCCACGAACTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGAACAGCCCGCAAGCATTGCGCGCCACCAAGGACTTGCTGCGCGAAGTGGACGACGGCGAACTCAGCCCGGCCCTGCGCCGTTACTGCGAAAACACCATC  >  AE015451/4591177‑4591640                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        aCCTGGATGATGCCCACGAACTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCgc                                                                                                                                                                                                                                       <  1:356739‑M2/235‑3 (MQ=255)         gatgCCCACGAACTGGGCGAGCTGATGTACGCCCTGCGTCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTa                                                                                                                                                                                                                              >  1:541957/1‑236 (MQ=255)            gCCCACGAACTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCgcgcgcc                                                                                                                                                                                                                                  >  2:531177‑M2/1‑222 (MQ=255)               cACGAACTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCCGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCt                                                                                                                                                                                                                        >  1:610838/1‑236 (MQ=255)                    aCTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCAcc                                                                                                                                                                                                                    <  1:275717/235‑1 (MQ=255)                                    tccGCCCTGCATCGCCTCAAGGCCCCGACCCTGGGCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCAccgcc                                                                                                                                                                                                                 <  2:683088/220‑1 (MQ=255)                                               tCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTgcgcg                                                                                                                                                                                           >  1:293657/1‑233 (MQ=255)                                                        ggCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCa                                                                                                                                                                                         >  1:282802/1‑226 (MQ=255)                                                                                        ccTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGc                                                                                                                                                                                                             >  2:787487/1‑174 (MQ=255)                                                                                                ggcgCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCgcgcgc                                                                                                                                                                                                                                   <  2:505696‑M2/144‑7 (MQ=255)                                                                                                     gcTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCAc                                                                                                                                   >  1:801780/1‑235 (MQ=255)                                                                                                     gcTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGCCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCt                                                                                                                                                                                                                        >  2:725011/1‑150 (MQ=255)                                                                                                         ggCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTgcgcg                                                                                                                                                                                           >  1:234723/1‑175 (MQ=255)                                                                                                                 cAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCa                                                                                                                                    <  2:406100/222‑1 (MQ=255)                                                                                                                                     tCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGaa                                                                                                   >  1:558710/1‑235 (MQ=255)                                                                                                                                       ggCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCgcgcgccgccaaagg                                                                                                                                                                                                                          >  1:36997‑M2/1‑99 (MQ=255)                                                                                                                                         cgcCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGG‑CAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGAACAGcc                                                                                              >  1:724372/1‑235 (MQ=255)                                                                                                                                                   gCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGAACAGCCCGCAAGCAtt                                                                                    <  1:84612/236‑1 (MQ=255)                                                                                                                                                            tgCCTGTCGCAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGAACAGCCCGCAAGc                                                                                       <  1:688360/224‑1 (MQ=255)                                                                                                                                                                         tACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCgcgcgccgccaaaggctgcaccttgcaccacggccagggtcggggccttgaggcgatgcagggcgtacatcagctcgcccagttcgtgggcatcatccaggttggtgttgaagtccagttgcgccgactgctgcatccaggccaggtcggcaccggcgctgaagtgccggc                                                              <  1:426241‑M2/236‑172 (MQ=255)                                                                                                                                                                            gCAGCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGAGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGc                                                                                                                           <  1:91857/172‑1 (MQ=255)                                                                                                                                                                                      ggCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGaa                                                                                                   >  1:351123/1‑186 (MQ=255)                                                                                                                                                                                          ccGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCgcgcgccgccaaaggctgcaccttgcaccacggccagggtcggggccttgaggcgatgcagggcgtacatcagctcgcccagttcgtgggcatcatccaggttggtgttgaagtccagttgcgccgactgctgcatccaggccaggtcggcaccggcgctgaagtgccggccacggccgcgcagcaga                                             <  2:554149‑M2/236‑189 (MQ=255)                                                                                                                                                                                           cggcggTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGaa                                                                                                   <  1:215426/181‑1 (MQ=255)                                                                                                                                                                                              aggTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGAACAGCCCGCAAGCATTGCGCGCCACCAAGGACTTGCTGCGCGAAGTGGACGACGGCGa                                          <  1:765798/234‑1 (MQ=255)                                                                                                                                                                                                gTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAAcc                                                                                                             >  2:660771/1‑166 (MQ=255)                                                                                                                                                                                                            gtCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGAACAGCCCGCAAGCATTGCGCGCCACCAAGGACTTGCTGCGCGAAGTGGACGACGGCGa                                          <  2:583931/220‑1 (MQ=255)                                                                                                                                                                                                              cGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGAACAGCCCGCAAGCATTGCGCGCCACCAAGGACTTGCTGCGCGAAGTgg                                                    >  2:765796/1‑209 (MQ=255)                                                                                                                                                                                                                        gCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGAACAGCCCGCAAGCATTGCGCGCCACCAAGGACTTGCTGCGCGAAGTGGACGACGGCGAACTCAGCCCGGCCCTGCGCCGTTACt                >  1:550503/1‑235 (MQ=255)                                                                                                                                                                                                                                       gccgcgcgccgccaaaggctgcaccttgcaccacggccagggtcggggccttgaggcgatgcagggcgtacatcagctcgcccagttcgtgggcatcatccaggttggtgttgaagtccagttgcgccgactgctgcatccaggccaggtcggcaccggcgctgaagtgccggccacggccgcgcagcagcacaaagcgcaggctggcgtcttcggccaactggtcgatggcca  <  1:456508‑M2/234‑232 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        ACCTGGATGATGCCCACGAACTGGGCGAGCTGATGTACGCCCTGCATCGCCTCAAGGCCCCGACCCTGGCCGTGGTGCAAGGTGCAGCCTTTGGCGGCGCGCTGGGCCTGATCAGCTGCTGCGACATGGCCATCGGCGCCGAAGACGCCCAACTGTGCCTGTCGGAAGTACGCATCGGCCTGGCCCCGGCGGTCATCAGCCCGTTCGTGGTCAAGGCCATTGGCGAACGCGCCGCGCGCCGCTACGCCCTCACCGCCGAGCGTTTCACTGGCGTGCGCGCACGCGAGCTGGGCCTGCTGGCCGAGGTGTACCCGGCCAGCGAACTGGACGACCACGTCGAAGCCTGGGTAAGCAACCTGCTGCAGAACAGCCCGCAAGCATTGCGCGCCACCAAGGACTTGCTGCGCGAAGTGGACGACGGCGAACTCAGCCCGGCCCTGCGCCGTTACTGCGAAAACACCATC  >  AE015451/4591177‑4591640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.