New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	4284707 =	29 (0.660)	3 (0.070)	3/324	NT	9.9%	coding (112/849 nt)	hbd	3‑hydroxybutyryl‑CoA dehydrogenase
	?	AE015451	4284752 =	27 (0.650)					coding (157/849 nt)	hbd	3‑hydroxybutyryl‑CoA dehydrogenase

GCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCAGCCAACGTCGCCGCCGACTGCCTGATTGCCACCAATACTTCGTCGCTGTCGGTGACCCAACTGGCCGCCAGCA  >  AE015451/4284526‑4284983                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        gCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCa                                                                                                                                                                                                                                   <  2:526253/233‑1 (MQ=255)    aCGATGTGTATCGGCGGTGGCCAAGGCGTCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAgg                                                                                                                                                                                                                             >  1:423061/1‑236 (MQ=255)                                 cGCCGTCGTGTACGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTc                                                                                                                                                                                                                                    <  1:142421/200‑1 (MQ=255)                                    aggatgcgctgcttgagctgcaggttctcggtagccgcctcgatcaccagctgtgcagcgctgagctgggtgtagtcggtgctggtacggatgcgggctttggcggctgcggccttgtcggcgtcgagagtgcccttgctgacctgGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAg                                                                                                                                                                                                                                  >  2:326059‑M1/147‑199 (MQ=255)                                          gtTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGc                                                                                                                                                                                                       >  2:665240/1‑220 (MQ=255)                                                    gTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAgccg                                                                                                                                                                                               <  1:147950/218‑1 (MQ=255)                                                                       aTGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCa                                                                                                                                                          <  1:704706/236‑1 (MQ=255)                                                                                         gCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAgg                                                                                                                                                                                                        >  2:712179/1‑172 (MQ=255)                                                                                         gCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGc                                                                                                                                                                                    <  2:51252/192‑1 (MQ=255)                                                                                         gCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGc                                                                                                                                                                                    >  1:51252/1‑192 (MQ=255)                                                                                              gATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGCCACGGCCGCAGCCGCCAAAGCCCGCACCCGTACCAGCACCGa                                                                                                                                                                   >  1:244457/1‑204 (MQ=255)                                                                                                                 ggCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGaa                                                                                                                >  1:51664/1‑236 (MQ=255)                                                                                                                    aaCGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAg                                                                                                                                                                         <  2:698492/176‑1 (MQ=255)                                                                                                                          aTTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCg                                                                                                                                                                    >  2:469805/1‑175 (MQ=255)                                                                                                                                    tgtgCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAgcgc                                                                                             <  1:698722/236‑1 (MQ=255)                                                                                                                                      tgCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCAt                                                                                           >  1:354132/1‑236 (MQ=255)                                                                                                                                         gcCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAACCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGcac                                                                                                                                            >  2:567377/1‑184 (MQ=255)                                                                                                                                                 cGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAggcggc                                                                                                                         >  1:244317/1‑195 (MQ=255)                                                                                                                                                                    gtggATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCAGCCAACGTcgccgc                                                             >  1:514671/1‑236 (MQ=255)                                                                                                                                                                         ccttgctgacctgGCGCTCGAGCGCGGTGTGGCCACCTTGAGCCAGAACCTCGAGCGcc                                                                                                                                                                                                                                         <  2:226886‑M1/46‑1 (MQ=255)                                                                                                                                                                              ctgacctggcgctcgaggttcttgctcaaggtggccacaccgcgctcgagcgcCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCt                                                                                        >  2:326059‑M2/54‑199 (MQ=255)                                                                                                                                                                              ccGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCAGCCAACGTCGCCGCCGACTGCCTg                                                   <  1:468601/236‑1 (MQ=255)                                                                                                                                                                                 aCGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCAGCCAACGTCGCCGCCGACTGCCTGAt                                                 <  1:507903/235‑1 (MQ=255)                                                                                                                                                                                   gCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCAGCCAACGTCGCCGCCGACTGCCTGATTGc                                              <  1:173524/236‑1 (MQ=255)                                                                                                                                                                                     ggcgctcgaggttctggctcaaggtggccacaccgcgctcgagcgcCAGGTCAGCAAgg                                                                                                                                                                                                                             <  2:226886‑M2/13‑1 (MQ=255)                                                                                                                                                                                        GCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCAGCCAACGTCGCCGCCGACTGCCTGATTGccacca                                         <  1:34601‑M1/236‑1 (MQ=255)                                                                                                                                                                                          tcgaggttcttgctcaaggtggccacaccgcgctcgagcgcCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCCGCCAACGTCGCCGCCGACTGCCTGa                                                  >  1:225883‑M2/42‑225 (MQ=255)                                                                                                                                                                                                gcgGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTac                                                                                                                                                               <  1:2596/110‑1 (MQ=255)                                                                                                                                                                                                gcgGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTac                                                                                                                                                               >  2:2596/1‑110 (MQ=255)                                                                                                                                                                                                               tGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCAGCCAACGTCGCCGCCGACTGCCTGATTGCCACCAATACTTCGTCGc                             >  2:454271/1‑225 (MQ=255)                                                                                                                                                                                                                 aGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCACAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAag                                                                                                   >  2:438667/1‑153 (MQ=255)                                                                                                                                                                                                                 aGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCga                                                                                                                   >  2:102492/1‑137 (MQ=255)                                                                                                                                                                                                                   cAAGAACCTCGAGCGCCAGGGCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGAGCGAGGCGGCCACCGAGAACCTg                                                                                                            >  2:184421/1‑142 (MQ=255)                                                                                                                                                                                                                           tcgagcgcCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCAGCCAACGTCGCCGCCGACTGCCTGATTGCCACCAATACTTCGTCGCTGTCGGTGACCCAACTGgccgcc      <  1:665242‑M2/228‑1 (MQ=255)                                                                                                                                                                                                                              agcgcCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCt                                                                                                                                                       <  2:178081‑M2/83‑1 (MQ=255)                                                                                                                                                                                                                               gcgcCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCAGCCAACGTCGCCGCCGACTGCCTGATTGCCACCAATACTTCGTCGCTGTCGGTGACCCAACTGGCCGCCAGCa  >  1:392016‑M2/5‑236 (MQ=255)                                                                                                                                                                                                                                cgcCAGGTCAGCAAGTGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCg                                                                                                                                                                               <  2:758302‑M2/59‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        GCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGCCAAAGCCCGCATCCGTACCAGCACCGACTACACCCAGCTCAGCGCTGCACAGCTGGTGATCGAGGCGGCTACCGAGAACCTGCAGCTCAAGCAGCGCATCCTGCAGCAGGTGGCAGCCAACGTCGCCGCCGACTGCCTGATTGCCACCAATACTTCGTCGCTGTCGGTGACCCAACTGGCCGCCAGCA  >  AE015451/4284526‑4284983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.