New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	5200909 =	32 (0.730)	3 (0.070)	3/332	NT	8.9%	coding (94/774 nt)	yafJ	putative amidotransferase
	?	AE015451	5200932 =	30 (0.710)					coding (71/774 nt)	yafJ	putative amidotransferase

CGAACGGGTGGGTGTTGGACAGGCACACCCTGCCGACGTTGGCCTGACGGATATGACCGATCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTCGACCCGGCCTGCGCCACGCGCAGCGGCTGGCGGCAAGTCGTCACGGTAACGGTCGCTGCGCTCGGCAGCCAGCGGCGCCTGGGTGCCGAGCTGGTCATCCTC  >  AE015451/5200720‑5201133                                                                                                                                                                                                                          |                                                                                                                                                                                                          cGAACGGGTGGGTGTTGGACAGGCACACCCTGCCGACGTTGGCCTGACGGATATGACCGATCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCgg                                                                                                                                                                                               <  1:296291/225‑1 (MQ=255)     cGGGTGGGTGTTGGACAGGCACACCCTGCCGACGTTGGCCTGACGGATATGACCGATCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAAc                                                                                                                                                                                  <  1:615433/234‑1 (MQ=255)                       gCACACCCTGCCGACGTTGGCCTGACGGATATGACCGATCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTgg                                                                                                                                                              <  1:346067/236‑1 (MQ=255)                                  cGACGTTGGCCTGACGGATATGACCGATCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGcc                                                                                                                                                    <  1:790896/235‑1 (MQ=255)                                   gACGTTGGCCTGACGGATATGACCGATCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGcc                                                                                                                                                    >  2:790894/1‑234 (MQ=255)                                      gTTGGCCTGACGGATATGACCGATCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCcagca                                                                                                                                               <  1:369299/236‑1 (MQ=255)                                                accgtgacgacgtgccgccagccgctgcgcgtggcgcaggccgggtcgagccccgtctgtaaacggagacacgcctatgtgtgaactgctgggcatgagtgccaacgtccctaccgacatcgttttgagcttcaccgtcctgatgcagcggggcgggcgaaccggcccgcTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGc                                                                                                                                                     <  1:435691‑M2/55‑1 (MQ=255)                                                        cccGATCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCAt                                                                                                                                                                                                       <  2:355142/161‑1 (MQ=255)                                                            aTCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCa                                                                                                                                                            >  1:435494/1‑201 (MQ=255)                                                            aTCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCa                                                                                                                                                            <  2:435492/201‑1 (MQ=255)                                                                 cacttcacttTGGATCGGGTAGCGCTGCACGAGGTGGGCCCCTTCCGACGCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTcaca                                                                                                                                        <  1:237716/216‑1 (MQ=255)                                                                    ttcacttTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTa                                                                                                                    >  2:747472/1‑233 (MQ=255)                                                                      cacttTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGGCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGGTGGCACTCATg                                                                                                                                                      >  2:362475/1‑197 (MQ=255)                                                                          tttGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTg                                                                                                                                                               >  1:264971/1‑184 (MQ=255)                                                                          tttGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCCCCCGCCCCGCTGCATCAGGCCGGt                                                                                                                                                                                              >  2:18637/1‑153 (MQ=255)                                                                            tGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACt                                                                                                                                                          >  2:472205/1‑187 (MQ=255)                                                                              gcaggccgggtcgagccccgtctgtaaacggagacacgcctatgtgtgaactgctgggcatgagtgccaacgtccctaccgacatcgttttcagcttcaccggcctgatgcagcggggcgggcgaaccggcccgcaccgcTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGt                                                                                                                                                                         <  2:777425‑M2/35‑1 (MQ=255)                                                                              aTCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTc                                                                                                        >  1:291459/1‑235 (MQ=255)                                                                                 gggTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGaaaa                                                                                                                                                                                   <  2:667167/157‑1 (MQ=255)                                                                                 gggTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATCGGCGTGTCCCCGtt                                                                                                                      >  2:366470/1‑218 (MQ=255)                                                                                 gggTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCACGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGaac                                                                                                                                                                                          >  2:210464/1‑149 (MQ=255)                                                                                                  gTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTc                                                                                                        <  1:8020/215‑1 (MQ=255)                                                                                                  ccccgtctgtaaacggagacacgcctacgtgtgaactgctgggcatgagtgccaacgtccctaccgacatcgttttcagcttcaccggcctgatgcagcggggcgggcgaaccggcccgcTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTgg                                                                                                                                                              <  1:572882‑M2/46‑1 (MQ=255)                                                                                                         aCTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCCCGACCCGGCCt                                                                                              >  2:506872/1‑218 (MQ=255)                                                                                                                 cTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTCGACCCGGCCTGCGCCACGCGCAGCGGCTGGCGGCa                                                                     <  1:505766/235‑1 (MQ=255)                                                                                                                        ctCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTGGGCa                                                                                                                                                            >  2:216506/1‑141 (MQ=255)                                                                                                                                   gTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGaa                                                                                                                                                                                           >  1:187279/1‑99 (MQ=255)                                                                                                                                       gcatgagtgccaacgtccctaccgacatcgttttcagcttcaccggcctgatgcaGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGACGGTGAAGCTCAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTCGACCCGGCCTGCGCCACGCGCAGCGGCTGGCGGCACGTCGTCACGGt                                                         <  1:435691‑M1/170‑1 (MQ=255)                                                                                                                                         caaCAGCCTCAGGCCGCGCCCTTCCTACAAGCCTTTCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGCTTCGCCCGCCCCGCTGCCTCACGCCGGTg                                                                                                                                                                                             <  2:447771/90‑1 (MQ=255)                                                                                                                                                ccaacgtccctaccgacatcgttttcagcttcaccggcctgatgcaGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACGTAGGCGTGTCTCCGTTTACAGACgggg                                                                                                           <  1:572882‑M1/120‑1 (MQ=255)                                                                                                                                                   aGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTCGACCCGGCCTGCGCCACGCGCAGCGGCTGGCGGCAAGTCGTCACGGTAACGGTCGCTGCGCTCGGcagc                                   >  1:3073/1‑235 (MQ=255)                                                                                                                                                       cgcgGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTG‑AAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTCGACCCGGCCt                                                                                              >  2:468842/1‑171 (MQ=255)                                                                                                                                                           accgacatcgttttcagcttcaccggcctgatgcaGCGGTGCGGGCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTCGACCCGGCCTgc                                                                                            <  2:777425‑M1/140‑1 (MQ=255)                                                                                                                                                            ccTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGcc                                                                                                                                                    >  2:297528/1‑113 (MQ=255)                                                                                                                                                                         cTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTCGACCCGGCCTGCGCCACGCGCAGCGGCTGGCGGCAAGTCGTCACGGTAACGGTCGCTGCGCTCGGCAGCCAGCGGCGCCTGGGTGCCGAGCt            >  1:355006/1‑236 (MQ=255)                                                                                                                                                                                    atgcagcGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTCGACCCGGCCTGCGCCACGCGCAGCGGCTGGCGGCAAGTCGTCACGGTAACGGTCGCTGCGCTCGGCAGCCAGCGGCGCCTGGGTGCCGAGCTGGTCATCCTc  <  1:777427/229‑1 (MQ=255)                                                                                                                                                                                                               gcccgcccCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTCGACCCGGCCTGCGCCACGCGCAGCGGCTGGCGGCAAGTCGTCACGGTAACGGTCGCTGCGCTCGGCAGCCAGCGGCGCCTGGGTGCCGAGCt            <  1:272806/203‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                          CGAACGGGTGGGTGTTGGACAGGCACACCCTGCCGACGTTGGCCTGACGGATATGACCGATCACCACTTCACTTTTGATCGGGTAGCGCTGCACGAGGTTGGCCACTTCCGACTCGCTGCTCGCGGCCGGGTCCTGGAACAGGCGCAGGCCGCGGCCTTCGTAAAAGCCTATCCCCCAGCCGTCACGGTGCGG‑‑‑‑‑GCCGGTTCGCCCGCCCCGCTGCATCAGGCCGGTGAAGCTGAAAACGATGTCGGTAGGGACGTTGGCACTCATGCCCAGCAGTTCACACATAGGCGTGTCTCCGTTTACAGACGGGGCTCGACCCGGCCTGCGCCACGCGCAGCGGCTGGCGGCAAGTCGTCACGGTAACGGTCGCTGCGCTCGGCAGCCAGCGGCGCCTGGGTGCCGAGCTGGTCATCCTC  >  AE015451/5200720‑5201133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.