New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	409939 =	35 (1.360)	3 (0.120)	3/302	NT	9.6%	coding (725/2646 nt)	aceE	Pyruvate dehydrogenase E1 component
	?	AE015451	410027 =	23 (0.940)					coding (637/2646 nt)	aceE	Pyruvate dehydrogenase E1 component

TGACTTCGTCCATGCGGCGCTGCAGGGCACCGTTGGTGTCCTTGGCCAGCAGTGGGTCCCAGAAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGG  >  AE015451/409727‑410147                                                                                                                                                                                                                      |                                                                                                                                                                                                                 tGACTTCGTCCATGCGGCGCTGCAGGGCACCGTTGGTGTCCTTGGCCAGCAGTGGGTCCCAGAAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGc                                                                                                                                                                                                     >  1:499211/1‑226 (MQ=255)                     tGCAGGGCACCGTTGGTGTCCTTGGCCAGCAGTGGGTCCCAGAAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTcc                                                                                                                                                                                  <  2:451540/225‑1 (MQ=255)                                 ttGGTGTCCTTGGCCAGCAGTGGGTCCCAGAAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGc                                                                                                                                                                               <  1:291211/216‑1 (MQ=255)                                  tGGTGTCCTTGGCCAGCAGTGGGTCCCAGAAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGcc                                                                                                                                                           >  1:443888/1‑235 (MQ=255)                                    gtgtCCTTGGCCAGCAGTGGGTCCCAGAAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCa                                                                                                                                                                                                         >  1:426475/1‑187 (MQ=255)                                     tgtCCTTGGCCAGCAGTGGGTCCCAGAAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATg                                                                                                                                                       >  1:516222/1‑236 (MQ=255)                                             gCCAGCAGTGGGTCCCAGCAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCGCCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCa                                                                                                                                                                                                         <  2:504615/178‑1 (MQ=255)                                               cagcagTGGGTCCCAGAAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCtcgtcg                                                                                                                                                                         >  2:383013/1‑208 (MQ=255)                                               cagcagTGGGTCCCAGAAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCtcgtcg                                                                                                                                                                         <  1:383017/208‑1 (MQ=255)                                                            caggaagtggacggcaacggcctgtcttcgtacccgcacccatggctgatgcctgacttctggcagttcccgaccgtttcgatgggtctgggcccgatccaggcgatctaccaggcacgcttcatgaagtacctggaagcccgtggcttcatcccggccggc                                                                                                                                                                                                           >  2:380562‑M1/155‑162 (MQ=255)                                                               aaaCGGCCCCAGACGACTTTGTTGACGGTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTgccg                                                                                                                               >  1:385108/1‑235 (MQ=255)                                                                             ggcTTCGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTcc                                                                                                                                                                                  <  2:278672/167‑1 (MQ=255)                                                                                  tgttgAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATg                                                                                                                                                       >  2:456515/1‑191 (MQ=255)                                                                                   gttgAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGGTGTCCAGCTTCTCGcggccgg                                                                                                                                                                                                            >  2:135232/1‑137 (MQ=255)                                                                                     tgAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAg                                                                                                                                                                                                        >  2:461270/1‑139 (MQ=255)                                                                                                   cccGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTgcc                                                                                                                                <  1:49090/197‑1 (MQ=255)                                                                                                          cGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACgg                                                                                                             >  1:165646/1‑210 (MQ=255)                                                                                                                gcGCCTTCGAGTTCCTGGATGCTCTTGCCGTTGCCGCGACCCGGGCCGTCGAGGCGCTGCAGGCTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTACGGCTCGTCGCACTCAc                                                                                                                                                                  <  2:86845/150‑1 (MQ=255)                                                                                                                        gAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCAc                                                                                                               <  2:119437/194‑1 (MQ=255)                                                                                                                         aGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTg                                                                    <  1:293140/236‑1 (MQ=255)                                                                                                                                ggATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCgg                                                              >  1:179937/1‑235 (MQ=255)                                                                                                                                     atCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGccc                                                          <  2:443884/234‑1 (MQ=255)                                                                                                                                        ttgccgttgccgCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGccc                                                          <  2:431903/231‑1 (MQ=255)                                                                                                                                         tgccgttgccgCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTcc                                                                                                                                                                                  >  1:134708/1‑110 (MQ=255)                                                                                                                                         tgccgttgccgCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTcc                                                                                                                                                                                  <  2:134704/110‑1 (MQ=255)                                                                                                                                                   gcgAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGCTGaa                                                                                                                    >  2:163461/1‑162 (MQ=255)                                                                                                                                                              cGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGc                               >  1:238439/1‑236 (MQ=255)                                                                                                                                                                   aGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAg                            <  2:179933/234‑1 (MQ=255)                                                                                                                                                                                   cttcatgaagtacctggaagcccgtggctacatccCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCAGGAAGCACCAGACCTTCTGc                                                                                                                                     <  1:313716‑M1/78‑1 (MQ=255)                                                                                                                                                                                    ttcatgaagtacctggaagcccgtgtctgcaaccCGGCCGGCCAGGGCGATTGCACCCAGGGATTcc                                                                                                                                                                                  <  1:326783‑M1/33‑1 (MQ=255)                                                                                                                                                                                      tgatgaCGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGc                                                            <  2:313269/183‑1 (MQ=255)                                                                                                                                                                                        atgaCGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATgg        <  2:238435/233‑1 (MQ=255)                                                                                                                                                                                           aCGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGa                                                                                                                                             >  1:57465/1‑97 (MQ=255)                                                                                                                                                                                               aGATCAGGTTGTCCAGCTTCTCGCCGCCGGCCAGGGCgct                                                                                                                                                                                                  >  2:417878/1‑38 (MQ=255)                                                                                                                                                                                                    aGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCgg  <  2:385104/227‑1 (MQ=255)                                                                                                                                                                                                    aGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATGGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCgg                                      <  2:117328/191‑1 (MQ=255)                                                                                                                                                                                                       ttGTCCAGCTCCCCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCGCGTCGCACTCACCGTCGccc                                                                                                                                                          <  2:57462/72‑1 (MQ=255)                                                                                                                                                                                                              gCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAg                                                        >  1:275970/1‑163 (MQ=255)                                                                                                                                                                                                              cttcatccCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTgccggc                                                                                                                             <  1:463980‑M1/86‑1 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                 TGACTTCGTCCATGCGGCGCTGCAGGGCACCGTTGGTGTCCTTGGCCAGCAGTGGGTCCCAGAAACGGCCCCAGACGACTTTGTTGAC‑GTTCCAGCCACCGCCACGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGG  >  AE015451/409727‑410147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.