New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	409939 =	35 (1.360)	3 (0.120)	3/302	NT	9.6%	coding (725/2646 nt)	aceE	Pyruvate dehydrogenase E1 component
	?	AE015451	410027 =	23 (0.940)					coding (637/2646 nt)	aceE	Pyruvate dehydrogenase E1 component

CGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGGGTACGAAGACAGGCCGTTGCCGTCCACTTCCTGGCGGAAGTTGTTCATCTGGTCTTCGCTGATACGGCCTTCCATGAAGGCACGGGCGTAGACGCCTGGAGATGCGTGGCCCT  >  AE015451/409831‑410260                                                                                                                                                                                                     |                                                                                                                                                                                                                                          cGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACgg                                                                                                                                                                                                                              >  1:165646/1‑210 (MQ=255)               gAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCAc                                                                                                                                                                                                                                <  2:119437/194‑1 (MQ=255)                aGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTg                                                                                                                                                                                     <  1:293140/236‑1 (MQ=255)                       ggATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCgg                                                                                                                                                                               >  1:179937/1‑235 (MQ=255)                            atCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGccc                                                                                                                                                                           <  2:443884/234‑1 (MQ=255)                               ttgccgttgccgCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGccc                                                                                                                                                                           <  2:431903/231‑1 (MQ=255)                                          gcgAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGCTGaa                                                                                                                                                                                                                                     >  2:163461/1‑162 (MQ=255)                                                     cGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGc                                                                                                                                                >  1:238439/1‑236 (MQ=255)                                                          aGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAg                                                                                                                                             <  2:179933/234‑1 (MQ=255)                                                                             tgatgaCGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGc                                                                                                                                                                             <  2:313269/183‑1 (MQ=255)                                                                               atgaCGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATgg                                                                                                                         <  2:238435/233‑1 (MQ=255)                                                                                           aGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCgg                                                                                                                   <  2:385104/227‑1 (MQ=255)                                                                                           aGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATGGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCgg                                                                                                                                                       <  2:117328/191‑1 (MQ=255)                                                                                                     gCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAg                                                                                                                                                                         >  1:275970/1‑163 (MQ=255)                                                                                                               gccggcaagcagaaggtctggtgcttcatgggcgacggtgagtgcgacgagccggaatccctgggtgcaatcgccctggccggccgGGATGAAg                                                                                                                                                                                                                                    <  1:463980‑M2/8‑1 (MQ=255)                                                                                                                       gcagaaggtctggtgcttcctgggcgacggtgagtgcgacgagccggaatccctgggtgcaatcgccctggccggccgGGATGTAGCCACGGGCTTCCAGGTACTTCATGAAg                                                                                                                                                                                                         <  1:313716‑M2/35‑1 (MQ=255)                                                                                                                                 aCCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCAGGAAGCACCAGGCCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGCTCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGCGAACTGCCAGAAGTCAg                                                                                                                                      <  1:518075/170‑1 (MQ=255)                                                                                                                                          ttCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGGGTACGAAGACAGGCCGTTGCCGTCCACTTCCTGGCGGACGTTGTTCATCTGGt                                                              >  2:238758/1‑233 (MQ=255)                                                                                                                                              ggCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCAGGCAGCGTGCCTAGTAGATGGCCt                                                                                                                                                                                      >  2:241065/1‑109 (MQ=255)                                                                                                                                                              cGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGt                                                                                                                       <  1:137651/156‑1 (MQ=255)                                                                                                                                                              cGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGt                                                                                                                       >  2:137647/1‑156 (MQ=255)                                                                                                                                                               gTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGGGTACGAAGACAGGCCg                                                                                                   <  2:516218/175‑1 (MQ=255)                                                                                                                                                                  gCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCTCAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGGGTACGAAAACAGGCCGGTGCAGTCCACTTCCTGGCGGAAGTTGTTCATCTGGTCTTCGCTGATa                                                   >  1:114611/1‑220 (MQ=255)                                                                                                                                                                    ggaatccctgggtgcaatcgccctggccggccgGGTTGCAGACACGGGCTTCCAGGTACTTCATGaa                                                                                                                                                                                                          <  1:326783‑M2/34‑1 (MQ=255)                                                                                                                                                                           gCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGGGTACGAAGACAGGCCGTTGCCGTCCACTTCCTGGCGGGAGTTGTTCATCTGGTCTTAGCTGATACGGCCTTCCATGAAGGCACGGGCg                           >  1:143806/1‑235 (MQ=255)                                                                                                                                                                                  aCCTTCTGCTGGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGGGTACGAAGACAGGCCGTTGCCGTCCACTTCCTGGCGGAAgttgtt                                                                      <  2:463976/185‑1 (MQ=255)                                                                                                                                                                                         gCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGGGTACGAAGACAGGCCGTTGCCGTCCACTTCCTGGCGGAAGTTGTTCATCTGGTCTTCGCTGATACGGCCTTCCATGAAGGCACGGGCGTAGACGCCTGGAGAt            >  1:306241/1‑236 (MQ=255)                                                                                                                                                                                             gccggccgGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGGGTACGAAGACAGGCCGTTGCCGTCCACTTCCTg                                                                                  >  2:380562‑M2/9‑162 (MQ=255)                                                                                                                                                                                                     ggATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGGGTACGAAGACAGGCCGTTGCCGTCCACTTCCTGGCGGAAGTTGTTCATCTGGTCTTCGCTGATACGGCCTTCCATGAAGGCACGGGCGTAGACGCCTGGAGATGCGTGGCCCt  >  2:297751/1‑234 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                                                                          CGGAACACGCCTTCGAGTTCCTGGATGATCTTGCCGTTGCCGCGAACCGGGCCGTCGAGGCGCTGCAGGTTGCAGTTGATGACGAAGATCAGGTTGTCCAGCTTCTCGCGGCCGGCCAGGGCGATTGCACCCAGGGATTCCGGCTCGTCGCACTCACCGTCGCCCATGAAGCACCAGACCTTCTGCTTGCCGGCCGGGATGAAGCCACGGGCTTCCAGGTACTTCATGAAGCGTGCCTGGTAGATCGCCTGGATCGGGCCCAGACCCATCGAAACGGTCGGGAACTGCCAGAAGTCAGGCATCAGCCATGGGTGCGGGTACGAAGACAGGCCGTTGCCGTCCACTTCCTGGCGGAAGTTGTTCATCTGGTCTTCGCTGATACGGCCTTCCATGAAGGCACGGGCGTAGACGCCTGGAGATGCGTGGCCCT  >  AE015451/409831‑410260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.