New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4158769	26 (1.010)	3 (0.120)	3/304	NT	11.8%	coding (779/1062 nt)	PP_3661	conserved membrane protein of unknown function, UPF0324 family
	?	AE015451	= 4158850	20 (0.810)					coding (860/1062 nt)	PP_3661	conserved membrane protein of unknown function, UPF0324 family

TGCATCTGGATACCATGGGCGCCGGGCTGCTGCTGGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCTGGACACCTTTGCCTTGACCATGGCCATGACGGCGTTGGGCATGGAGACCCGCTTCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGC  >  AE015451/4158568‑4158999                                                                                                                                                                                                          |                                                                                                                                                                                                                                       tgCATCTGGATACCATGGGCGCCGGGCTGCTGCTGGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGtt                                                                                                                                                                                                       >  1:147696/1‑235 (MQ=255)            aCCATGGGCGCCGGGCTGCTGCTGGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCt                                                                                                                                                                                                   >  2:510710/1‑228 (MQ=255)            aCCATGGGCGCCGGGCTGCTGCTGGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTACTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGc                                                                                                                                                                                             <  2:389416/234‑1 (MQ=255)             ccATGGGCGCCGGGCTGCTGCTGGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCtt                                                                                                                                                                                           <  1:510714/235‑1 (MQ=255)                  ggCGCCGGGCTGCTGCTGGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCg                                                                                                                                                                                                     >  2:442151/1‑220 (MQ=255)                  ggCGCCGGGCTGCTGCTGGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCt                                                                                                                                                                                            <  1:57457/229‑1 (MQ=255)                            tgctgctgGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGgc                                                                                                                                                                                                                          <  2:471898/189‑1 (MQ=255)                            tgctgctgGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGgc                                                                                                                                                                                                                          >  1:471902/1‑189 (MQ=255)                              ctgctgGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCcctggtcct                                                                                                                                                                            <  2:122518/233‑1 (MQ=255)                                    ggcggcACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCagcacttgcatcgaattcaccaggaccagggccaggaagccgaaagcg                                                                                                                                                                                        <  1:176135‑M1/215‑49 (MQ=255)                                     gcggcACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCtt                                                                                                                                                                                           <  1:118782/211‑1 (MQ=255)                                                 aCGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCagcacttgcatcgaattcaccaggaccagggccaggaagccga                                                                                                                                                                                             >  2:176131‑M1/1‑154 (MQ=255)                                                      gTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTgcctg                                                                                                                                                  <  1:463719‑M2/235‑6 (MQ=255)                                                           gcAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTg                                                                                                                                                                        >  1:30589/1‑208 (MQ=255)                                                           gcAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGACCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTgcctgg                                                                                                                                                 <  2:497039‑M2/231‑7 (MQ=255)                                                                                        gCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCt                                                                                                                      <  2:429906/229‑1 (MQ=255)                                                                                                                 caTCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGc                                                                                                                                                              <  2:366129/164‑1 (MQ=255)                                                                                                                         aGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCTGGaca                                                                                                                 <  1:464900/201‑1 (MQ=255)                                                                                                                         aGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCTGGACACCTTTGCCTTGACCATGGCCa                                                                                            <  2:147692/222‑1 (MQ=255)                                                                                                                           aTGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGa                                                                                                                                         <  2:321191/175‑1 (MQ=255)                                                                                                                                                     ggTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGATTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGag                                                                                                                                                                >  2:150021/1‑125 (MQ=255)                                                                                                                                                          tgctgGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGa                                                                                                                                         >  2:278797/1‑144 (MQ=255)                                                                                                                                                                    gggCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCTGGACACCTTTGCCTTGACCATGGCCATGACg                                                                                       >  1:372472/1‑184 (MQ=255)                                                                                                                                                                       ccTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCTGGACACCTTTGCCTTGACCATGGCCATGACGGCGTTGGGCATGGAGACCCGCTTCAGCCAGATTCGCCAGGCTGGGCCACggg                                   >  1:333266/1‑233 (MQ=255)                                                                                                                                                                           tgGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCTGGACACCTTTGCCTTGACCATGGCCa                                                                                            <  2:374889/172‑1 (MQ=255)                                                                                                                                                                                 aGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCTGGACACCTTTGCCTTGACCATGGCCATGACGGCGTTGGGCATGGAGACCCGCTTCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCg                       <  1:497043/235‑1 (MQ=255)                                                                                                                                                                                    cGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCTGGACACCTTTGCCTTGACCATGGCCATGACGGCGTTGGGCATGGAGACCCGCTTCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTg                    <  1:442155/235‑1 (MQ=255)                                                                                                                                                                                     gTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCTGGACACCTTTGCCTTGACCATGGCCATGACGGCGTTGGGCATGGAGACCCGCTTCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGc                   >  1:188272/1‑235 (MQ=255)                                                                                                                                                                                      ttCGCGCAAGGCAGGCCAGGCagcacttgcatcgaattcaccaggaccagggccaggaagccgaaagcgaaccagggcatggcaatgcgcccgttgccctg                                                                                                                                                        >  2:397270‑M1/1‑21 (MQ=255)                                                                                                                                                                                           gcAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCcctg                                                                                                                                                                                 <  2:150457/71‑1 (MQ=255)                                                                                                                                                                                           gcAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCcctg                                                                                                                                                                                 >  1:150461/1‑71 (MQ=255)                                                                                                                                                                                                     ccaggcagcacttgcatcgaattcaccaggaccagggccaggaagccgaaagcgaaccaggtcatggcaatgcgcccgttgccctgcgcctggcctgccttgcgcgaacggctgatccacaggcccaccaccagcagtaccggcaccagcagcatgacccgggtcatcttgacgatggtggcgacatgcgtggcttcggggctgacattgctggccgcgcctaccacctgc       <  2:497039‑M1/231‑226 (MQ=255)                                                                                                                                                                                                      caggcagcacttgcatcgaattcaccaggaccagggccaggaagccgaaagcgaaccagggcatggcaatgcgcccgttgccctgcgcctggcctgccttgcgcgaacggctgatccacaggcccaccaccagcagtaccggcaccagcagcatgacccgggtcatcttgacgatggtggcgacatgcgtggcttcggggctgacattgctggccgcgcctaccacctgcgccac  <  1:463719‑M1/235‑231 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                                       TGCATCTGGATACCATGGGCGCCGGGCTGCTGCTGGGCGGCACTGTCCACGAAGTGGCGCAGGTGGTAGGCGCGGCCAGCAATGTCAGCCCCGAAGCCACGCATGTCGCCACCATCGTCAAGATGACCCGGGTCATGCTGCTGGTGCCGGTACTGCTGGTGGTGGGCCTGTGGATCAGCCGTTCGCGCAAGGCAGGCCAGGCGCAGGGCAACGGGCGCATTGCCATGCCCTGGTTCGCTTTCGGCTTCCTGGCCCTGGTCCTGGTGAATTCGATGCAAGTGCTGCCTGGCAGCGTGACCCAGGCCGTGAACAGCCTGGACACCTTTGCCTTGACCATGGCCATGACGGCGTTGGGCATGGAGACCCGCTTCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGC  >  AE015451/4158568‑4158999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.