New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	3158934 =	42 (0.730)	5 (0.090)	5/326	NT	11.0%	coding (953/2097 nt)	opuA	glycine betaine ABC transporter, ATPase/permease fusion protein
	?	AE015451	3158952 =	41 (0.750)					coding (971/2097 nt)	opuA	glycine betaine ABC transporter, ATPase/permease fusion protein

CCTGACTCACCAGTAGCAGCGCCAAGGGTAAAGCGACACCCGGTGAGTCACGGCCGTGGCCGTGCAGGCTCACCCAAGCACAACCCAAGCGATTGACCAGCAGCACTGCCAGTGCTGTGGCCGATAGCCACCCCGCCCCCTCGCGCCACGCGCCAGCAAGCATGGCCAGGGCCGCCACCTCCACCAGCAGCGCCAGCCAGAACCAACCACCTCGTCCGGCGGCAGCGAGCGCTGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/3159170‑3158934 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgctgccgCCGGACGAGGTGGTTGGTTCTGGCTGGCGCTGCTGGTGGAGGTGGCGGCCCTGGCCATGCTTGCTGGCGCGTGGCGCGAGGGGGCGGGGTGGCTATCGGCCACAGCACTGGCAGTGCTGCTGGTCAATCGCTTGGGTTGTGCTTGGGTGAGCCTGCACGGCCACGGCCGTGACTCACCGGGTGTCGCTTTACCCTTGG  >  AE015451/3158952‑3159149                                                                                                                                                                                                                                                                                                                                                                                                                                                     CCTGACTCACCAGTAGCAGCGCCAAGGGTAAAGCGACACCCGGTGAGTCACGGCCGTGGCCGTGCAGGCTCACCCAAGCACAACCCAAGCGATTGACCAGCAGCACTGCCAGTGCTGTGGCAGATAGCCACCCCGCCCCCTCGCGCCACGCGCCAGCAAGCATGGCCAGGGCCGCCACCTCCACCAGCAGCGCCAGCCAGAACCAACCACCTCGTCCGGCGGCAGCGAGCGCTGC                                                                                                                                                                                                          >  1:746270/1‑235         ACCAGTAGCAGCGCCAAGGGTAAAGCGACACCCGGTGAGTCACGGCCGTGGCCGTGCAGGCTCACCCAAGCACAACCCAAGCGATTGACCAGCAGCACTGCCAGTGCTGTGGCCGATAGCCACCCCGCCCCCTCGCGCCACGCGCCAGCAAGCATGGCCAGGGCCGCCACCTCCACCAGCAGCGCCAGCCAGAACCAACCACCTCGTCCGGCGGCAGCGAGCGCTGCCGCCGGAC                                                                                                                                                                                                  <  1:686716/235‑1                                                                                                          CTGCCAGTCCTGTGGCCGATAGCCACCCCGCCCCCTCGCGCCACGCGCCAGCAAGCATGGCCAGGGCCGCCACCTCCACCAGCAGCGCCAGCCAGAACCAACCACCTCGTCCGGCGGCAGCGAGCGCT                                                                                                                                                                                                            <  1:259998/128‑1                                                                                                               AGTGCTGTGGCCGATAGCCACCCCGCCCCCTCGCGCCACGCGCCAGCAAGCATGGCCAGGGCCGCCACCTCCACCAGCAGCGCCAGCCAGAACCAACCACCTCGTCCGGCGGCAGCGAGCGCTGCCGCCGGACGAGGTGGTTGGTTCTGGCTG                                                                                                                                                                              <  2:449556/153‑1                                                                                                                                CCACCCCGCCCCCTCGCGCCACGCGCCAGCAAGCATGGCCAGGGCCGCCACCTCCACCAGCAGCGCCAGCCAGAACCACCCACCTCGTCCGGCGGCAGCGAGCGCTGCCGCCGGACGAGGTGGTTGGTTCTGGC                                                                                                                                                                                <  2:794961/134‑1                                                                                                                                                          CAGCAAGCATGGCCAGGGCCGCCACCTCCACCAGCAGCGCCAGCCAGAACCAACCACCTCGTCCGGCGGCAGCGAGCGCTGCCGCCGGACGAGGTGGTTGGTTCTGGCTGGCGCTGCTGGTGGAGGTGGCGGCCCTGGCCATGCTTGCTGGCGCGTGGCGCGAGGGGGCGGGGTGGCTATCGGCCACAGCACTGGCAGTGCTGCTGGTCAATCGCCTGGGTTGTGCTTGGGTGA                                                  >  1:651659/1‑234                                                                                                                                                                  agGGCCAGGGCCGCCACCTCCACCAGCAGCGCCAGCCAGAACCAACCACCTCGTCCGGCGGCAGCGAGCGCTGCCGCCGGACGAGGTGGTTGGTTCTGGCTGGCGCTGCTGGTGGAGGTGGCGGCCCTGGCCATGCTTGCTGGCGCGTGGCGCGAGGGGGCGGGGTGG                                                                                                            <  1:27098/166‑1                                                                                                                                                                                                                                      CGCTGCCGCCGGACGAGGTGGTTGGTTCTGGCTGGCGCTGCTGGTGGAGGTGGCGGCCCTGGCCATGCTTGCTGGCGCGTGGCGCGAGGGGGCGGGGTGGCTATCGGCCACAGCACTGGCAGTGCTGCTGGTCAATCGCTTGGGTTGTGCTTGGGTGAGCCTGCACGGCCACGGCCGTGACTCACCGGGTGTCGCTTTACCCTTGG  <  2:610295/206‑1                                                                                                                                                                                                                                        CTGCCGCCGGACGAGGTGGTTGGTTCTGGCTGGCGCTGCTGGTGGAGGTGGCGGCCCTGGCCATGCTTGCTGGCGCGTGGCGCGAGGGGGCGGGGTGGCTATCGGCCACAGCACTGGCAGTGCTGCTGGTCAATCGCTTGGGTTGTGCTTGGGTGAGCCTGCACGGCCACGGCCGTGACTCACCGG                    <  1:858838/186‑1                                                                                                                                                                                                                                        CTGCCGCCGGACGAGGTGGGTGGTTCGGGCTGGCGCTGCTGGTGGAGGTGGCGGCCCTGGCCATGCTTGCTGGCGCGTGGCGCGAGGGGGCGGGGTGGCTATCGGCCACAGCACTGGCAGTGCTGCTG                                                                              <  2:115335/128‑1                                                                                                                                                                                                                                             GCCGGACGAGGTGGTTGGTTCTGGCTGGCGCTGCTGGTGGAGGTGGCGGCCCTGGCCATGCTTGCTGGCGCGTGGCGCGAGGGGGCGGGGTGGCTATCGGCCACAGCACTGGCAGTGCTGCTGGTCAATCGCTTGGGTTGTGCTT                                                        <  2:1086423/145‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                     CCTGACTCACCAGTAGCAGCGCCAAGGGTAAAGCGACACCCGGTGAGTCACGGCCGTGGCCGTGCAGGCTCACCCAAGCACAACCCAAGCGATTGACCAGCAGCACTGCCAGTGCTGTGGCCGATAGCCACCCCGCCCCCTCGCGCCACGCGCCAGCAAGCATGGCCAGGGCCGCCACCTCCACCAGCAGCGCCAGCCAGAACCAACCACCTCGTCCGGCGGCAGCGAGCGCTGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/3159170‑3158934 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgctgccgCCGGACGAGGTGGTTGGTTCTGGCTGGCGCTGCTGGTGGAGGTGGCGGCCCTGGCCATGCTTGCTGGCGCGTGGCGCGAGGGGGCGGGGTGGCTATCGGCCACAGCACTGGCAGTGCTGCTGGTCAATCGCTTGGGTTGTGCTTGGGTGAGCCTGCACGGCCACGGCCGTGACTCACCGGGTGTCGCTTTACCCTTGG  >  AE015451/3158952‑3159149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.