Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	6,077,996	+TACT	8.7%	coding (1230/1290 nt)	PP_5330 ←	Major facilitator family transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 6077996	43 (0.750)	4 (0.070) +TACT	3/334	NT	8.7%	coding (1230/1290 nt)	PP_5330	Major facilitator family transporter
	?	AE015451	6077997 =	43 (0.750)					coding (1229/1290 nt)	PP_5330	Major facilitator family transporter

AGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/6077912‑6077996 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ACGTAGTAGG‑CAG‑GCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGC  >  AE015451/6077997‑6078130                                                                                      ||||                                                                                                                                         AGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCTACTACGTGGCAGGCCTGTGCGCCGTCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCC                                                    >  1:560708/1‑175 AGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCTACTACGTGGCAGGCCTGTGCGCCGTCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCC                                                    <  2:560705/175‑1                                                                        cgatggcGCCTGCCTACTACGT‑‑‑‑GG‑CAG‑GCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGC  >  1:861853/8‑148                                                                        cgatggcGCCTGCCTACTACGT‑‑‑‑GG‑CAG‑GCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGC  <  2:861850/141‑1                                                                                      ||||                                                                                                                                         AGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/6077912‑6077996 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ACGTAGTAGG‑CAG‑GCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGC  >  AE015451/6077997‑6078130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.