Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	6,077,996	+TACT	8.7%	coding (1230/1290 nt)	PP_5330 ←	Major facilitator family transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 6077996	43 (0.750)	4 (0.070) +TACT	3/334	NT	8.7%	coding (1230/1290 nt)	PP_5330	Major facilitator family transporter
	?	AE015451	6077997 =	43 (0.750)					coding (1229/1290 nt)	PP_5330	Major facilitator family transporter

AATACGATATGACTTCATTGTGACAATTGGATGAAAGGCCATCACTGGAAGCAGGTCTGGCCTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCACTGCGCCAATCACGCCCACGCACAGGCCGGTCACGGCATACAGCGGGAACAGCAACTCGGGACGGGT  >  AE015451/6077765‑6078217                                                                                                                                                                                                                                        |                                                                                                                                                                                                                              aagaCGATATGACTTCATTGTGACAATTGGATGAAAGGCCATCACTGGAAGCAGGTCTGGCCTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACgt                                                                                                                                                                                                                           <  1:337138/233‑1 (MQ=255)           gACTTCATTGTGACAATTGGATGAAAGGCCATCACTGGAAGCAGGTCTGGCCTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTaggcag                                                                                                                                                                                                                  >  1:124068/1‑235 (MQ=255)                 aTTGTGACAATTGGATGAAAGGCCATCACTGGAAGCAGGTCTGGCCTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACg                                                                                                                                                                                                                            >  1:309696/1‑219 (MQ=255)                   tgtgACAATTGGATGAAAGGCCATCACTGGAAGCAGGTCTGGCCTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCg                                                                                                                                                                                                         <  1:634462/236‑1 (MQ=255)                     tgACAATTGGATGAAAGGCCATCACTGGAAGCAGGTCTGGCCTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCGGCCACGTAGTAGGCAGGCGCCATCgg                                                                                                                                                                                                        >  1:157229/1‑235 (MQ=255)                                 gAAAGGCCATCACTGGAAGCAGGTCTGGCCTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTcagc                                                                                                                                                                                           <  1:438958/236‑1 (MQ=255)                                           cACTGGAAGCAGGTCTGGCCTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGtagtag                                                                                                                                                                                                                      <  2:701405/199‑1 (MQ=255)                                           cACTGGAAGCAGGTCTGGCCTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGt                                                                                                                                                                                   <  2:640715/234‑1 (MQ=255)                                                             ccTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCGGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGc                                                                                                                                                                      >  1:139271/1‑229 (MQ=255)                                                               tctTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGc                                                                                                                                                                                      <  2:366400/211‑1 (MQ=255)                                                                     cgGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAg                                                                                                                                                       >  1:597342/1‑236 (MQ=255)                                                                                  tCCTACAGGGGGTATGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGTCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGcc                                                                                                                                           <  1:582813/235‑1 (MQ=255)                                                                                  gccTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTAGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGcc                                                                                                                                           <  2:642645/234‑1 (MQ=255)                                                                                    cTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGcc                                                                                                                                           <  2:597339/233‑1 (MQ=255)                                                                                      aCAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAgg                                                                                                                                             <  1:626374/229‑1 (MQ=255)                                                                                                  cacaGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCCGCAGTGCGGTCACCCCCATCGGCGTCAGCCCGCCa                                                                                                                                                          >  2:212794/1‑204 (MQ=255)                                                                                                  cacaGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCATCCCg                                                                                                                                                             >  1:119133/1‑201 (MQ=255)                                                                                                         cTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGtagtag                                                                                                                                                                                                                      <  1:1039276/137‑1 (MQ=255)                                                                                                         cTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGtagtag                                                                                                                                                                                                                      >  2:1039273/1‑137 (MQ=255)                                                                                                         cTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGGTGTACGAGAACGACAACCCg                                                                                                                   >  1:226008/1‑236 (MQ=255)                                                                                                          tCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCg                                                                                                                   >  1:880790/1‑235 (MQ=255)                                                                                                               gCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCGGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTcagca                                                                                                                                                                                          >  2:266160/1‑159 (MQ=255)                                                                                                                     acCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTTTCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTCGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTcagc                                                                                                                                                                                           <  2:1029366/152‑1 (MQ=255)                                                                                                                                  gAGCGGCTTTAGGCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCAGCTTCAGCAGTGCGGTcacc                                                                                                                                                                               >  1:1029369/1‑151 (MQ=255)                                                                                                                                  gAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAATCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATc                                                                                                                                                                                                          >  1:139462/1‑124 (MQ=255)                                                                                                                                  gAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACg                                                                                                                                 >  1:407640/1‑197 (MQ=255)                                                                                                                                       gCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGc                                                                                         <  2:569805/232‑1 (MQ=255)                                                                                                                                        cTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTc                                                                                                                                                                                                    >  1:1036737/1‑124 (MQ=255)                                                                                                                                        cTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTc                                                                                                                                                                                                    <  2:1036734/124‑1 (MQ=255)                                                                                                                                                    agagGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCtactacgtggcaggcctgtgcgccgtcgggctcaccttcagcagtgcggtcaccaccatcggcgtcagcccgccaaagatcgcgtaggcc                                                                                                                                     >  1:560708‑M1/1‑85 (MQ=255)                                                                                                                                                    agagGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCtactacgtggcaggcctgtgcgccgtcgggctcaccttcagcagtgcggtcaccaccatcggcgtcagcccgccaaagatcgcgtaggcc                                                                                                                                     <  2:560705‑M1/175‑91 (MQ=255)                                                                                                                                                                ccGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCACTGCGCCa                                                             >  1:759257/1‑235 (MQ=255)                                                                                                                                                                             cAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGAc                                                                             >  2:627432/1‑206 (MQ=255)                                                                                                                                                                                    tgtggGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCACTGCGCCAATCACGCCCACGCACAGGCCg                                        <  1:386829/236‑1 (MQ=255)                                                                                                                                                                                     gttgGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCACTGCGCCAATCACGCCCACGCACAGGCCgg                                       <  1:789530/236‑1 (MQ=255)                                                                                                                                                                                            aGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCACTGCGCCAATCACGCCCACGCACAGGCCGGTCACgg                                 <  1:747295/235‑1 (MQ=255)                                                                                                                                                                                                       gCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGcc                                                                                                                                           <  2:252184/118‑1 (MQ=255)                                                                                                                                                                                                            cGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCa                                                                     >  2:608565/1‑183 (MQ=255)                                                                                                                                                                                                              gCTAGACCGCCGGCGCACAGGCCTGCCCCGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGa                                                                                    <  1:211556/166‑1 (MQ=255)                                                                                                                                                                                                              gCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCg                                                                                                                                                                                                         <  2:192359/49‑1 (MQ=255)                                                                                                                                                                                                              gCTAGACCGACGGCGCACAGGCCCGCCACGTAGTAGGCAGGCGCCATCg                                                                                                                                                                                                         >  1:192361/1‑49 (MQ=255)                                                                                                                                                                                                                     cGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCACt                                                                   >  2:235039/1‑176 (MQ=255)                                                                                                                                                                                                                          gcgcACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCACTGCGCCAATCACGCCCACGCACAGGCCGGTCACGGCATACAGCGGGAACAGCAACTCGGGACGGGt  >  1:236397/1‑236 (MQ=255)                                                                                                                                                                                                                          gcgcACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCCCACCTTCAGCAGTGCGGTCACCACCATCGGCGGCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGACCGCCtt                                                                                      >  2:931971/1‑152 (MQ=255)                                                                                                                                                                                                                           cgatggcgcctgcctactacgtggcaggcgccatcgggctcaccttcagcagtgcggtcaccaccatcggcgtcagcccgccaaagatcgcgtaggccacgttgtacgagaacgacaacccgctgaaacgcaccaccgccgggaacgc                                                                                         >  1:861853‑M1/8‑14 (MQ=255)                                                                                                                                                                                                                           cgatggcgcctgcctactacgtggcaggcgccatcgggctcaccttcagcagtgcggtcaccaccatcggcgtcagcccgccaaagatcgcgtaggccacgttgtacgagaacgacaacccgctgaaacgcaccaccgccgggaacgc                                                                                         <  2:861850‑M1/141‑135 (MQ=255)                                                                                                                                                                                                                           cgatggcgCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCACTGCGCCAATCACGCCCACGCACAGGCCGGt                                      >  2:531977/8‑199 (MQ=255)                                                                                                                                                                                                                            gcACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCatc                                                                   >  2:559377/1‑167 (MQ=255)                                                                                                                                                                                                                             cacaGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCAccgccg                                                                                                <  2:966449/139‑1 (MQ=255)                                                                                                                                                                                                                                      gCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGCTCGCGTAGGCCACGCTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGa                                                                                    <  2:531128/142‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                              AATACGATATGACTTCATTGTGACAATTGGATGAAAGGCCATCACTGGAAGCAGGTCTGGCCTCTTCGCGGCTAAAGCCGTTCCTACAGGGGGTTTGCACAGGCCTCAAGGCCGACACCTAACCTGTAGGAGCGGCTTTAGCCGCGAAGAGGCCAGTAGCCGAAGCGCAGATCAGCGCTTGTTGGCGAGCAGGTACAGGCCCACGGCTAGACCGACGGCGCACAGGCCTGCCACGTAGTAGGCAGGCGCCATCGGGCTCACCTTCAGCAGTGCGGTCACCACCATCGGCGTCAGCCCGCCAAAGATCGCGTAGGCCACGTTGTACGAGAACGACAACCCGCTGAAACGCACCACCGCCGGGAACGCCTTGACCATGACATAAGGCACTGCGCCAATCACGCCCACGCACAGGCCGGTCACGGCATACAGCGGGAACAGCAACTCGGGACGGGT  >  AE015451/6077765‑6078217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.