Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	303,121	+GCT	41.7%	coding (1286/1314 nt)	envZ →	Osmolarity sensor protein EnvZ

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 303121	22 (0.410)	16 (0.280) +GCT	15/314	NT	41.7%	coding (1286/1314 nt)	envZ	Osmolarity sensor protein EnvZ
	?	AE015451	303122 =	22 (0.380)					coding (1287/1314 nt)	envZ	Osmolarity sensor protein EnvZ

CGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCGACGTTCTTGCCGGTGGTGACCTCGATGCCCTCCAG  >  AE015451/302896‑303354                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          cgcCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATC‑TCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctg                                                                                                                                                                                                                                 <  1:682938‑M1/235‑11 (MQ=255)                   gtgtGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGa                                                                                                                                                                                                               >  1:845865/1‑236 (MQ=255)                              cGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGc                                                                                                                                                                                                    >  1:635000/1‑236 (MQ=255)                               ggggCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCtgt                                                                                                                                                                                                                   >  1:605882/1‑220 (MQ=255)                                gggCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACt                                                                                                                                                                                                       <  2:644569/231‑1 (MQ=255)                                   cACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTAAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCctct                                                                                                                                                                                                >  1:593942/1‑235 (MQ=255)                                       ggTATTGACGAGGCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCtgtgt                                                                                                                                                                                                                 <  2:1054256/214‑1 (MQ=255)                                        gTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgcc                                                                                                                                                                                                                            <  2:489997‑M1/202‑16 (MQ=255)                                                      gAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgca                                                                                                                                                                            >  1:982042‑M1/1‑173 (MQ=255)                                                           ggAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                                                        <  2:634997/235‑1 (MQ=255)                                                           ggAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGAGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                                                        <  1:457196/235‑1 (MQ=255)                                                               aCCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCa                                                                                                                                                                                             >  1:713783/1‑210 (MQ=255)                                                                             gTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCAcc                                                                                                                                                                             <  2:729092/212‑1 (MQ=255)                                                                             gTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCAcc                                                                                                                                                                             >  1:729095/1‑212 (MQ=255)                                                                                 aTTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTgg                                                                                                                                                    >  2:574710/1‑233 (MQ=255)                                                                                     gcgGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacag                                                                                                                                                                     <  1:897793‑M1/212‑71 (MQ=255)                                                                                           aTCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAg                                                                                                                                                                                    <  2:1025770/191‑1 (MQ=255)                                                                                           aTCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAg                                                                                                                                                                                    >  1:1025773/1‑191 (MQ=255)                                                                                              gggCACGGGGCGGCAATGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacg                                                                                                                                                                                        <  1:231643‑M1/184‑52 (MQ=255)                                                                                              gggCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCcgc                                                                                                                                     >  1:1010127/1‑235 (MQ=255)                                                                                                      ggcggcAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAgcggcg                                                                                                                            >  1:1050646/1‑236 (MQ=255)                                                                                                        cggcAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacg                                                                                                                                                                                        >  1:1003861‑M1/1‑123 (MQ=255)                                                                                                               ggCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAgc                                                                                                                                <  2:845862/223‑1 (MQ=255)                                                                                                                    cGGGCTGGGGCTGGCGCTCGGCACGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcact                                                                                                                                                                                                    <  1:348983‑M1/150‑40 (MQ=255)                                                                                                                                    aTCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgc                                                                                                <  2:1025110‑M1/234‑140 (MQ=255)                                                                                                                                    aTCGTCAAGCGGATTGCTGCGCAACAAGGCGGGATCGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaat                                                                                                                                                                                                                     <  2:304644‑M1/117‑23 (MQ=255)                                                                                                                                        tCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGGTGCCGCTGGGGCTGCTGCTGCCGCGGAATGCtgg                                                                                                                                                                                                                   >  1:241467/1‑114 (MQ=255)                                                                                                                                                           aCATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGc                                                                        <  2:1013099/235‑1 (MQ=255)                                                                                                                                                            cATGGCGGGAACGTGGAGTTGCGTAACCGGTCTTGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTggg                                                                                                                                                                                                                                           >  1:142888/1‑71 (MQ=255)                                                                                                                                                            cATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctg                                                                                                                                                                                                                                 <  2:79118‑M1/81‑11 (MQ=255)                                                                                                                                                                         tGGAGTGGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                                                        <  2:632391/125‑1 (MQ=255)                                                                                                                                                                           gAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcgcggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgccctt                                                                                            <  2:937667‑M1/199‑144 (MQ=255)                                                                                                                                                                           gAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGt                                                         <  2:1050643/234‑1 (MQ=255)                                                                                                                                                                                 cGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagcc                                                                                                                                                                               <  2:629391‑M1/110‑61 (MQ=255)                                                                                                                                                                                     aCCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggttaaagccgcacctacagggattgcgcagtacctgtggca                                                                                                                                               >  2:586268‑M1/1‑46 (MQ=255)                                                                                                                                                                                               cggcggGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGt                                                         <  1:27907/214‑1 (MQ=255)                                                                                                                                                                                                 gcggGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtccgggtctgattgggcccgccattcttctccaggtgctcgatgatcatcccggcg                                  >  1:818777‑M1/1‑34 (MQ=255)                                                                                                                                                                                                    ggATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtccgggtctgattgggcccgccattcttctccaggtgctcgatgatcatcccggcgac                                <  2:818774‑M1/234‑204 (MQ=255)                                                                                                                                                                                                       tCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttga                                                                                                            >  2:738602‑M1/1‑28 (MQ=255)                                                                                                                                                                                                                          gCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggcttgcgcagtccctgtggcagcggcgtggccg                                                                                                                                   >  2:796885‑M1/1‑9 (MQ=255)                                                                                                                                                                                                                               tGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtccgggtctgattgggcccgccattcttctccaggtgctcgatgatcatcccggcgacgttcttgccggtggtgacctcgatgccc    >  1:979959‑M1/1‑4 (MQ=255)                                                                                                                                                                                                                               tGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAg                                                           >  2:395383/1‑180 (MQ=255)                                                                                                                                                                                                                                  ggctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtccgggtctgattgggcccgccattcttctccaggtgctcgatgatcatcccggcgacgttcttgccggtggtgacctcgatgccctc  >  2:378078‑M1/1‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          CGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCGACGTTCTTGCCGGTGGTGACCTCGATGCCCTCCAG  >  AE015451/302896‑303354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.