Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	303,121	+GCT	41.7%	coding (1286/1314 nt)	envZ →	Osmolarity sensor protein EnvZ

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 303121	22 (0.410)	16 (0.280) +GCT	15/314	NT	41.7%	coding (1286/1314 nt)	envZ	Osmolarity sensor protein EnvZ
	?	AE015451	303122 =	22 (0.380)					coding (1287/1314 nt)	envZ	Osmolarity sensor protein EnvZ

TGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCGACGTTCTTGCCGGTGGTGACCTCGATGCCCTC  >  AE015451/302894‑303351                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      cgccgtatgtggtgctcagtgtgctggaccggggcaccggtattgacgagtcggagctggaaaccatctcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctgctgctg                                                                                                                                                                                                                                 <  1:682938‑M2/7‑1 (MQ=255)                     gtgtGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGa                                                                                                                                                                                                            >  1:845865/1‑236 (MQ=255)                                cGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGc                                                                                                                                                                                                 >  1:635000/1‑236 (MQ=255)                                 ggggCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCtgt                                                                                                                                                                                                                >  1:605882/1‑220 (MQ=255)                                  gggCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACt                                                                                                                                                                                                    <  2:644569/231‑1 (MQ=255)                                     cACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTAAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCctct                                                                                                                                                                                             >  1:593942/1‑235 (MQ=255)                                       gtattgacgagtcggagctggaaaccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGcc                                                                                                                                                                                                                            <  2:489997‑M2/12‑1 (MQ=255)                                         ggTATTGACGAGGCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCtgtgt                                                                                                                                                                                                              <  2:1054256/214‑1 (MQ=255)                                                     gagctggaaaccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCa                                                                                                                                                                            >  1:982042‑M2/177‑236 (MQ=255)                                                             ggAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                                                     <  2:634997/235‑1 (MQ=255)                                                             ggAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGAGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                                                     <  1:457196/235‑1 (MQ=255)                                                                 aCCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCa                                                                                                                                                                                          >  1:713783/1‑210 (MQ=255)                                                                               gTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCAcc                                                                                                                                                                          >  1:729095/1‑212 (MQ=255)                                                                               gTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCAcc                                                                                                                                                                          <  2:729092/212‑1 (MQ=255)                                                                                   aTTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTgg                                                                                                                                                 >  2:574710/1‑233 (MQ=255)                                                                                    gcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                                                     <  1:897793‑M2/67‑1 (MQ=255)                                                                                             gggcacggggcggcaatggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACg                                                                                                                                                                                        <  1:231643‑M2/48‑1 (MQ=255)                                                                                             aTCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAg                                                                                                                                                                                 >  1:1025773/1‑191 (MQ=255)                                                                                             aTCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAg                                                                                                                                                                                 <  2:1025770/191‑1 (MQ=255)                                                                                                gggCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCcgc                                                                                                                                  >  1:1010127/1‑235 (MQ=255)                                                                                                       cggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACg                                                                                                                                                                                        >  1:1003861‑M2/127‑174 (MQ=255)                                                                                                        ggcggcAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAgcggcg                                                                                                                         >  1:1050646/1‑236 (MQ=255)                                                                                                                 ggCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAgc                                                                                                                             <  2:845862/223‑1 (MQ=255)                                                                                                                   cgggctggggctggcgctcggcacgcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACt                                                                                                                                                                                                    <  1:348983‑M2/36‑1 (MQ=255)                                                                                                                                   atcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGc                                                                                                <  2:1025110‑M2/136‑1 (MQ=255)                                                                                                                                   atcgtcaagcggattgctgcgcaacaaggcgggatcgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAAt                                                                                                                                                                                                                     <  2:304644‑M2/19‑1 (MQ=255)                                                                                                                                          tCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGGTGCCGCTGGGGCTGCTGCTGCCGCGGAATGCtgg                                                                                                                                                                                                                >  1:241467/1‑114 (MQ=255)                                                                                                                                                           catggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctgctgctg                                                                                                                                                                                                                                 <  2:79118‑M2/7‑1 (MQ=255)                                                                                                                                                             aCATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGc                                                                     <  2:1013099/235‑1 (MQ=255)                                                                                                                                                                          gagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCGCGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCtt                                                                                            <  2:937667‑M2/140‑1 (MQ=255)                                                                                                                                                                           tGGAGTGGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                                                     <  2:632391/125‑1 (MQ=255)                                                                                                                                                                             gAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGt                                                      <  2:1050643/234‑1 (MQ=255)                                                                                                                                                                                cgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGcc                                                                                                                                                                               <  2:629391‑M2/57‑1 (MQ=255)                                                                                                                                                                                    accggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGTTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCa                                                                                                                                               >  2:586268‑M2/50‑138 (MQ=255)                                                                                                                                                                                                gcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCg                                  >  1:818777‑M2/38‑235 (MQ=255)                                                                                                                                                                                                 cggcggGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGt                                                      <  1:27907/214‑1 (MQ=255)                                                                                                                                                                                                   ggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCGAc                                <  2:818774‑M2/200‑1 (MQ=255)                                                                                                                                                                                                      tcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGa                                                                                                            >  2:738602‑M2/32‑155 (MQ=255)                                                                                                                                                                                                                         gccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGCTTGCGCAGTCCCTGTGGCAGCGGCGTGGCcg                                                                                                                                   >  2:796885‑M2/13‑113 (MQ=255)                                                                                                                                                                                                                              tggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCGACGTTCTTGCCGGTGGTGACCTCGATGccc    >  1:979959‑M2/8‑235 (MQ=255)                                                                                                                                                                                                                                 tGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAg                                                        >  2:395383/1‑180 (MQ=255)                                                                                                                                                                                                                                 ggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCGACGTTCTTGCCGGTGGTGACCTCGATGCCCTc  >  2:378078‑M2/5‑234 (MQ=255)                                                                                                                                                                                                                                   ctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCGACGTTCTTGCCg                      <  2:1071048‑M2/210‑1 (MQ=255)                                                                                                                                                                                                                                   ctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCGACGTTCTTGCCg                      >  1:1071051‑M2/3‑212 (MQ=255)                                                                                                                                                                                                                                     gctgctgctGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCGACGTTCTTGCCGGTGGTGACCTc           <  2:979956/221‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      TGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCGACGTTCTTGCCGGTGGTGACCTCGATGCCCTC  >  AE015451/302894‑303351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.