Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	4,775,320	Δ160 bp	5.6%	coding (3788‑3947/10413 nt)	pvdD ←	non‑ribosomal peptide synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4775319	53 (0.920)	3 (0.060)	3/318	NT	5.6%	coding (3948/10413 nt)	pvdD	non‑ribosomal peptide synthetase
	?	AE015451	4775480 =	51 (0.950)					coding (3787/10413 nt)	pvdD	non‑ribosomal peptide synthetase

ACCTGGGTATTGACGAAGAAGCCCACCACCCCTTCGGTTTCGCCGCGCGTGCGATTGGCCACCGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATC  >  AE015451/4775092‑4775550                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        aCCTGGGTATTGACGAAGAAGCCCACCACCCCTTCGGTTTCGCCGCGCGTGCGATTGGCCACCGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTg                                                                                                                                                                                                                                 <  1:927309/236‑1 (MQ=255)     aggTATTGACGAAGAAGCCCACCACCCCTTCGGTTTCGCCGCGCGTGCGATTGGCCACCGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGcc                                                                                                                                                                                                                             <  2:638109/235‑1 (MQ=255)                     gCCCACCACCCCTTCGGTTTCGCCGCGCGTGCGATTGGCCACCGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGcc                                                                                                                                                                                                                             <  2:844217/220‑1 (MQ=255)                       ccaccaCCCCTTCGGTTTCGCCGCGCGTGCGATTGGCCACCGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAgtcggtc                                                                                                                                                                                                           <  1:1063515/236‑1 (MQ=255)                                cTTCGGTTTCGCCGCGCGTGCGATTGGCCACCGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGgcagt                                                                                                                                                                                                                                    <  2:398288‑M1/202‑6 (MQ=255)                                          gccgcGCGTGCGATTGGCCACCGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACGCCCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCt                                                                                                                                                                                                                                  <  1:329084/195‑1 (MQ=255)                                          gccgcGCGTGCGATTGGCCACCGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGc                                                                                                                                                                                         >  1:827745/1‑235 (MQ=255)                                                cgTGCGATTGGCCACCGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGAGGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGACCAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTg                                                                                                                                                                                                                                     <  2:296423/185‑1 (MQ=255)                                                             agcGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCt                                                                                                                                                                                        <  2:390969/215‑1 (MQ=255)                                                              ccGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGCTCGGCAATTCCAGCACCGGCTGATCACCGCCCAACtgtg                                                                                                                                                                      <  2:595031/234‑1 (MQ=255)                                                                 gaaCGGCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCt                                                                                                                                                                                        <  2:368120/211‑1 (MQ=255)                                                                  cACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTgcgc                                                                                                                                                                                                                              >  2:631410/1‑174 (MQ=255)                                                                       ccACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCGGCGCCGGGCGCGGGCAGTCGGTCGGCTAtgcac                                                                                                                                                                                                 >  1:178102/1‑194 (MQ=255)                                                                            cgGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAg                                                                                                                                                      >  1:636441/1‑236 (MQ=255)                                                                              gATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCt                                                                                                                                                                                        <  1:63986/200‑1 (MQ=255)                                                                                  tCCGGCTGAGTCGAGTAACGAGGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCGGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTg                                                                                                                                                                                                                                     <  2:231484/151‑1 (MQ=255)                                                                                            tCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTgctcg                                                                                                                                      >  1:605604/1‑236 (MQ=255)                                                                                               aGTAACGATGAAGCAGGGTCTGGAGGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAgtcggtc                                                                                                                                                                                                           <  2:38189/164‑1 (MQ=255)                                                                                                  aaCGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTc                                                                                                                                                                  >  1:829582/1‑202 (MQ=255)                                                                                                    cGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGgcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatc                                                                                                                              <  2:738351‑M1/236‑108 (MQ=255)                                                                                                         aaGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTgctc                                                                                                                                       <  2:605601/222‑1 (MQ=255)                                                                                                         aaGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTg                                                                                                                                          >  2:928639/1‑219 (MQ=255)                                                                                                            cAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCcg                                                                                                                                                >  2:681638/1‑210 (MQ=255)                                                                                                                  cTGGAAGCTGGCCAGCAGCAACATGGACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGGGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAgtcggtc                                                                                                                                                                                                           >  1:975443/1‑145 (MQ=255)                                                                                                                       ggcgggCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCATGCCCCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAgtcggtc                                                                                                                                                                                                           <  2:272204/136‑1 (MQ=255)                                                                                                                            gCCAGCGGCAGCATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGt                                                                                                                                                                                      <  2:124839/156‑1 (MQ=255)                                                                                                                            gCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCCCCgg                                                                                                                                                                                          >  2:48874/1‑152 (MQ=255)                                                                                                                            gCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCcgc                                                                                                      >  1:538577/1‑236 (MQ=255)                                                                                                                             ccAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCt                                                                                                                                                                                        <  2:915351/153‑1 (MQ=255)                                                                                                                             ccAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCt                                                                                                                                                                                        >  1:915354/1‑153 (MQ=255)                                                                                                                              cagcagCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGGTGcaatacgccga                                                                                                    <  1:1014983/236‑11 (MQ=255)                                                                                                                              cagcagCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGt                                                                                                    <  1:358077/236‑1 (MQ=255)                                                                                                                                    caACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCcgcg                                                                                                                                              >  2:654715/1‑188 (MQ=255)                                                                                                                                         tGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGc                                                                                                              <  1:707925/215‑1 (MQ=255)                                                                                                                                         tGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGc                                                                                                              >  2:707922/1‑215 (MQ=255)                                                                                                                                         tGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCg                                                                                            >  2:378515/1‑233 (MQ=255)                                                                                                                                              aGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGc                                                                                                                          >  1:246193/1‑198 (MQ=255)                                                                                                                                                   gACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGt                                                                                                 >  1:155967/1‑218 (MQ=255)                                                                                                                                                   gACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCAc                                                                                    >  2:884512/1‑231 (MQ=255)                                                                                                                                                   gACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGCGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCCCGCCTCCAGCCATTGGCGCTGcct                                                                                                            >  1:189852/1‑206 (MQ=255)                                                                                                                                                    aCG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCg                                                                                  >  2:673494/1‑232 (MQ=255)                                                                                                                                                     cG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTgctcg                                                                                                                                      >  2:1062206/1‑179 (MQ=255)                                                                                                                                                         cctgggcctgggCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGgcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatcaccagcacatgcgcctgggcctcaaggcgcaaaaggtcaacgcggatcagtg                                                                          >  1:495580‑M1/1‑77 (MQ=255)                                                                                                                                                         cctgggcctgggCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCCTGGCTGCGCCGGGCGCGGGTAgtcg                                                                                                                                                                                                              >  2:816801/1‑104 (MQ=255)                                                                                                                                                              gcctgggcctgCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTAt                                                                                         >  2:523937/1‑216 (MQ=255)                                                                                                                                                              gcctgggcctgCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGGCGGTCGGCATTTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCa                                                                                                                                                               >  1:218143/1‑146 (MQ=255)                                                                                                                                                                                 gTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGgcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatcaccagcacatgcgcctgggcctcaaggcgcaaaaggtcaacgcggatcagtgggccgttgaccaggtcgaagg                                                     <  2:495577‑M1/233‑181 (MQ=255)                                                                                                                                                                                 gTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCGATTCCAGCACCGGCTGTTCACCGCCCa                                                                                                                                                                            >  2:949125/1‑114 (MQ=255)                                                                                                                                                                                   gCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGAc                                                 <  1:76381/235‑1 (MQ=255)                                                                                                                                                                                    ccTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGa                                               <  1:486952/236‑1 (MQ=255)                                                                                                                                                                                            ggcggcgCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTgctc                                                                                                                                       <  2:247459/139‑1 (MQ=255)                                                                                                                                                                                                       cAGCGCCAGGCTCAACTGGCCGCCGGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCCCCGCCCAACTGTGCTTCCCAGTAGGCCGGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGcc                                                       <  1:246487/209‑1 (MQ=255)                                                                                                                                                                                                        aGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGt                                                                    >  1:762221/1‑195 (MQ=255)                                                                                                                                                                                                                      cTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAg                                                                                                  <  2:378578/151‑1 (MQ=255)                                                                                                                                                                                                                       tGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCa             <  1:681641/235‑1 (MQ=255)                                                                                                                                                                                                                       gggCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGAc                                                               <  2:792079/184‑1 (MQ=255)                                                                                                                                                                                                                           cgccAGCGCGGgcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatcaccagcacatgcgcctgggcctcaaggcgcaaaaggtcaacgcggatcagtgggccgttgaccaggtcgaagggttcacgggccaa                                        <  1:769058‑M1/204‑194 (MQ=255)                                                                                                                                                                                                                            gccAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACg                                                              <  2:384713/181‑1 (MQ=255)                                                                                                                                                                                                                             ccAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTg                                       >  2:411746/1‑203 (MQ=255)                                                                                                                                                                                                                             ccAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTg                                       <  1:411749/203‑1 (MQ=255)                                                                                                                                                                                                                              cAGCGCGGgcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatcaccagcacatgcgcctgggcctcaaggcgcaaaaggtcaacgcggatcagtgggccgttgaccaggtcgaagggttcacgggccaactgctcgaccgtggcctgcaattcgacag           <  2:479078‑M1/230‑223 (MQ=255)                                                                                                                                                                                                                              cAGCGCGGgcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatcaccagcacatgcgcctgggcctcaaggcgcaaaaggtcaacgcggatcagtgggccgttgaccaggtcgaagggttcacgggccaactgctcgaccgtggcctgcaattcgacag           >  1:479081‑M1/1‑8 (MQ=255)                                                                                                                                                                                                                               gtcatgggcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatcaccagcacatgcgcctgggcctcaaggcgcaaaaggtcaacgcggatcagtgggccgttgaccaggtcgaagggttcacgggcccactgctcgaccgt                            >  1:753277‑M1/6‑7 (MQ=255)                                                                                                                                                                                                                                gcgcGGgcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatcaccagcacatgcgcctgggcctcaaggcgcaaaaggtcaacgcggatcagtgggccgttgaccaggtcgaagggttcacgggccaactgctcgaccgtggcctgcaattcgacagcatcgcc    >  1:113815‑M1/1‑6 (MQ=255)                                                                                                                                                                                                                                 cgcgGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATc  <  1:921893/236‑1 (MQ=255)                                                                                                                                                                                                                                   cgGgcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatcaccagcacatgcgcctgggcctcaaggcgcaaaaggtcaac                                                                                     <  2:189850‑M1/151‑149 (MQ=255)                                                                                                                                                                                                                                   cgGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGc                                                                                                                          <  1:281448/114‑1 (MQ=255)                                                                                                                                                                                                                                   cgGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGAc                                                 <  2:391349/187‑1 (MQ=255)                                                                                                                                                                                                                                     ggcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatcaccagcacatgcgcctgggcctcaaggcgcaaaaggtcaacgcggatcagtgggccgttgaccaggtcgaagggttcacgggccaactgctcgacc                              <  1:438690‑M1/204‑204 (MQ=255)                                                                                                                                                                                                                                     ggcagttcgacggcctgcccatgacgatagccctggtacagcgccacgagctcgttgaccagcaccgccatcgaccagccatcggtgatgatgtggtgcaggttcatcaccagcacatgcgcctgggcctcaaggcgcaaaaggtcaacgcggatcagtgggccgttgaccaggtcgaagggttcacgggccaactgctcgacc                              >  2:438687‑M1/1‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        ACCTGGGTATTGACGAAGAAGCCCACCACCCCTTCGGTTTCGCCGCGCGTGCGATTGGCCACCGGCACGCCCACGCGGATGTCCGGCTGAGTCGAGTAACGATGAAGCAGGGTCTGGAAGCTGGCCAGCAGCAACATGAACAGGGTGACG‑CCCTGGGCCTGGGCCTGCCGCCTGAGTGCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATC  >  AE015451/4775092‑4775550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.