Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	4,775,320	Δ160 bp	5.6%	coding (3788‑3947/10413 nt)	pvdD ←	non‑ribosomal peptide synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4775319	53 (0.920)	3 (0.060)	3/318	NT	5.6%	coding (3948/10413 nt)	pvdD	non‑ribosomal peptide synthetase
	?	AE015451	4775480 =	51 (0.950)					coding (3787/10413 nt)	pvdD	non‑ribosomal peptide synthetase

GCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCAACTGCTCGACCGTGGCCTGCAATTCGACAGCATCGAC  >  AE015451/4775269‑4775708                                                                                                                                                                                                                    |                                                                                                                                                                                                                                     gCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGAc                                                                                                                                                                                                               <  1:76381/235‑1 (MQ=255)  ccTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGa                                                                                                                                                                                                             <  1:486952/236‑1 (MQ=255)               cttcggtttcgccgcgcgtgcgattggccaccggcacgcccacgcggatgtccggctgagtcgagtaacgatgaagcagggtctggaagctggccagcagcaacatgaacagggtgacgccctgggcctgggcctgccgcctgagtgcctgcaccagcggcgcgtccagcgccaggctcaactggccgccagcgcggGCAGt                                                                                                                                                                                                                                  <  2:398288‑M2/5‑1 (MQ=255)                     cAGCGCCAGGCTCAACTGGCCGCCGGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCCCCGCCCAACTGTGCTTCCCAGTAGGCCGGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGcc                                                                                                                                                                                                                     <  1:246487/209‑1 (MQ=255)                      aGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGt                                                                                                                                                                                                                                  >  1:762221/1‑195 (MQ=255)                                     tGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCa                                                                                                                                                                           <  1:681641/235‑1 (MQ=255)                                     gggCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGAc                                                                                                                                                                                                                             <  2:792079/184‑1 (MQ=255)                                          gccAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACg                                                                                                                                                                                                                            <  2:384713/181‑1 (MQ=255)                                           ccAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTg                                                                                                                                                                                                     >  2:411746/1‑203 (MQ=255)                                           ccAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTg                                                                                                                                                                                                     <  1:411749/203‑1 (MQ=255)                                               cgcgGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATc                                                                                                                                                                <  1:921893/236‑1 (MQ=255)                                                 cgGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGAc                                                                                                                                                                                                               <  2:391349/187‑1 (MQ=255)                                                       ggCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGc                                                                                                                                                         <  1:884515/235‑1 (MQ=255)                                                         cTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGc                                                                                                                                                         <  2:827742/233‑1 (MQ=255)                                                            cgcCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCgg                                                                                                                                                   <  1:922428/236‑1 (MQ=255)                                                                 ggCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGtgatg                                                                                                                                              <  1:673497/236‑1 (MQ=255)                                                                    gcgGGTAGGCGGTCGGCAAGTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGc                                                                                                                                                                                     <  2:872902/194‑1 (MQ=255)                                                                       ggTAGTCGGTCGGCAATTCCCGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAg                                                                                                                                                          <  1:287215/218‑1 (MQ=255)                                                                       ggTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGAc                                                                                                                                                                                                               <  2:696287/165‑1 (MQ=255)                                                                       ggTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGAtgtg                                                                                                                                         <  1:422228/235‑1 (MQ=255)                                                                                   cgatgaagcagggtctggaagctggccagcagcaacatgaacagggtgacgccctgggcctgggcctgccgcctgagtgcctgcaccagcggcgcgtccagcgccaggctcaactggccgccagcgcggGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTtcatc                                                                                                                            <  2:738351‑M2/107‑1 (MQ=255)                                                                                             cACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGcaca                                                                                                                   <  1:748989/235‑1 (MQ=255)                                                                                                 ggCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATgcg                                                                                                               <  1:702255/235‑1 (MQ=255)                                                                                                     gTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGAc                                                                                                                                                                                                               >  2:883675/1‑135 (MQ=255)                                                                                                     gTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGAc                                                                                                                                                                                                               <  1:883678/135‑1 (MQ=255)                                                                                                     gTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGt                                                                                                                                                  >  2:11232/1‑196 (MQ=255)                                                                                                     gTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCtt                                                                                                           >  2:1007474/1‑234 (MQ=255)                                                                                                              ccAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGa                                                                                                                                                              >  2:1035572/1‑175 (MQ=255)                                                                                                                     tgCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCaaaa                                                                                          <  1:631413/236‑1 (MQ=255)                                                                                                                        ttCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAgg                                                                                        >  2:739106/1‑235 (MQ=255)                                                                                                                          cccAGGAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTCCACCGCCCGCGCGGGCAGTTCGACGGCCCGCCCATggc                                                                                                                                                                                                               >  2:102509/1‑112 (MQ=255)                                                                                                                               tAGGCCAGCTGCCGCGCTTGCTCGCTGGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATGGCGCCGCCAGCGCGGGCAGTGCGACGGCCTGCCCAGGACGATAGCCCTGGTACAGCGCCACGAGCTCGtt                                                                                                                                                                                <  2:38013/140‑1 (MQ=255)                                                                                                                                  gCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGc                                                                                                                      <  2:670864/195‑1 (MQ=255)                                                                                                                                  gCCAGCTGCCGCGCTTGCGCGCTCGCCCCCAGCCATGGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGc                                                                                                                                                                                     <  2:155965/132‑1 (MQ=255)                                                                                                                                     aGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCa                                                                           <  1:523940/235‑1 (MQ=255)                                                                                                                                       cctgggcctgggcctgccgcctgagtgcctgcaccagcggcgcgtccagcgccaggctcaactggccgccagcgcggGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTg                                                                        >  1:495580‑M2/78‑236 (MQ=255)                                                                                                                                       cTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATg                                                                                                                 <  1:775813/195‑1 (MQ=255)                                                                                                                                       cTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATg                                                                                                                 >  2:775810/1‑195 (MQ=255)                                                                                                                                       cTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTg                                                                        >  1:476136/1‑236 (MQ=255)                                                                                                                                         gCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAg                                                                                         >  1:946843/1‑217 (MQ=255)                                                                                                                                          ccGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGAc                                                                                                                                                                                                                             <  1:240115/84‑1 (MQ=255)                                                                                                                                          ccGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGAc                                                                                                                                                                                                                             >  2:240112/1‑84 (MQ=255)                                                                                                                                          ccGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTgg                                                                                                                                                                                                    >  2:831196/1‑109 (MQ=255)                                                                                                                                          ccGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTgg                                                                                                                                                                                                    <  1:831199/109‑1 (MQ=255)                                                                                                                                               cTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGgc                                                                                                >  2:829266/1‑204 (MQ=255)                                                                                                                                                             tCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGAAGTGCTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAg                                                    >  1:573319/1‑234 (MQ=255)                                                                                                                                                               gtgcctgcaccagcggcgcgtccagcgccaggctcaactggccgccagcgcggGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAgg                                                   <  2:495577‑M2/180‑1 (MQ=255)                                                                                                                                                                     ttGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACggg                                          <  1:928642/236‑1 (MQ=255)                                                                                                                                                                               cAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGtt                                                                                                                                >  2:105164/1‑140 (MQ=255)                                                                                                                                                                               cAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCAACt                                    >  2:957174/1‑232 (MQ=255)                                                                                                                                                                                     gcgTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGca                                                                                                                     >  2:973827/1‑145 (MQ=255)                                                                                                                                                                                           tCGGGGTATTGCCCCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGc                                                                                                                                                         <  2:318811/103‑1 (MQ=255)                                                                                                                                                                                           tCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATgtggt                                                                                                                                       >  1:815464/1‑121 (MQ=255)                                                                                                                                                                                           tCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATgtggt                                                                                                                                       <  2:815461/121‑1 (MQ=255)                                                                                                                                                                                           tCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCAACTGCTCGACCGTGGCCTg                    >  1:918641/1‑236 (MQ=255)                                                                                                                                                                                              gCGTATGGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGAc                                                                                                                                                             <  2:118614/96‑1 (MQ=255)                                                                                                                                                                                                         cgccagcgcggGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCaa                                      <  1:769058‑M2/193‑1 (MQ=255)                                                                                                                                                                                                            cagcgcggGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCAACTGCTCGACCGTGGCCTGCAATTCGACAg         <  2:479078‑M2/222‑1 (MQ=255)                                                                                                                                                                                                            cagcgcggGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCAACTGCTCGACCGTGGCCTGCAATTCGACAg         >  1:479081‑M2/9‑230 (MQ=255)                                                                                                                                                                                                             gtcatggGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCCACTGCTCGACCGt                          >  1:753277‑M2/8‑212 (MQ=255)                                                                                                                                                                                                              gcgcggGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCAACTGCTCGACCGTGGCCTGCAATTCGACAGCAtcgcc  >  1:113815‑M2/7‑233 (MQ=255)                                                                                                                                                                                                                 cggGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAAc                                                                                   <  2:189850‑M2/148‑1 (MQ=255)                                                                                                                                                                                                                   gGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCAACTGCTCGAcc                            >  2:438687‑M2/2‑204 (MQ=255)                                                                                                                                                                                                                   gGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCAACTGCTCGAcc                            <  1:438690‑M2/203‑1 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                     GCCTGCACCAGCGGCGCGTCCAGCGCCAGGCTCAACTGGCCGCCAGCGCGGCTTGGCTGCGCCGGGCGCGGGTAGTCGGTCGGCAATTCCAGCACCGGCTGTTCACCGCCCAACTGTGCTTCCCAGTAGGCCAGCTGCCGCGCTTGCTCGCTCGCCTCCAGCCATTGGCGCTGCCAAGCCGCGTAGTCGGCGTATTGCACCGCCAGCGCGGGCAGTTCGACGGCCTGCCCATGACGATAGCCCTGGTACAGCGCCACGAGCTCGTTGACCAGCACCGCCATCGACCAGCCATCGGTGATGATGTGGTGCAGGTTCATCACCAGCACATGCGCCTGGGCCTCAAGGCGCAAAAGGTCAACGCGGATCAGTGGGCCGTTGACCAGGTCGAAGGGTTCACGGGCCAACTGCTCGACCGTGGCCTGCAATTCGACAGCATCGAC  >  AE015451/4775269‑4775708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.