New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 2529011	31 (0.780)	3 (0.080)	3/316	NT	10.5%	intergenic (+138/‑269)	PP_2217/PP_2218	enoyl‑CoA hydratase/transposase
	?	AE015451	= 4908014	22 (0.590)					coding (11/198 nt)	PP_4317	conserved protein of unknown function

CTTTCTCCACCGAAGACCAGAAAGAAGGCATGGCCGCATTCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCTTCGGTAGAATTCGCTGAAATCCAGTCAGGAAGACCCGAGCGATGTGGGCTGA  >  AE015451/2528797‑2529239                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     cTTTCTCCACCGAAGACCAGAAAGAAGGCATGGCCGCATTCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAcctgcctccgcaaggcctg                                                                                                                                                                                                                   >  1:261854‑M1/1‑215 (MQ=255)    tctcCACCGAAGACCAGAAAGAAGGCATGGCCGCATTCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCaa                                                                                                                                                                                                                                     >  2:516315/1‑213 (MQ=255)     ctcCACCGAAGACCAGAAAGAAGGCATGGCCGCATTCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGc                                                                                                                                                                                                             <  1:451297/236‑1 (MQ=255)      tcCACCGAAGACCAGAAAGAAGGCATGGCCGCATTCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGc                                                                                                                                                                                                             >  1:478298/1‑235 (MQ=255)           cGAAGACCAGAAAGAAGGCATGGCCGCATGCATCGCCAAGCGGGAGGCACAGTTCAAGGCCCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCt                                                                                                                                                                                                                                <  2:169336/211‑1 (MQ=255)                                  ccGCATTCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGGCGGGCATGGATGCCCGTCAAGCGCTGAGGccc                                                                                                                                                                                                                         >  2:24439/1‑195 (MQ=255)                                  ccGCATTCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCatg                                                                                                                                                                                                                     >  2:554293/1‑199 (MQ=255)                                  ccGCATTCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCatg                                                                                                                                                                                                                     <  1:554294/199‑1 (MQ=255)                                      aTTCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAcctgcctccgcaaggcctgcgttcggcctcctga                                                                                                                                                                                                    >  1:459533‑M1/1‑178 (MQ=255)                                        tCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCg                                                                                                                                                                          <  1:516316/236‑1 (MQ=255)                                          ctCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAgcg                                                                                                                                                                                                     <  1:281636/206‑1 (MQ=255)                                                     tGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAgcgc                                                                                                                                                                                                    >  2:93359/1‑197 (MQ=255)                                                             aGTTCAAGGACCGTGGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAgc                                                                                                                                                      <  1:432160/235‑1 (MQ=255)                                                                tCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGCGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATgggg                                                                                                                                                                                                              <  1:526725/176‑1 (MQ=255)                                                                tCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCGTGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGGACTCCCGGGa                                                                                                                                                                                        >  2:89469/1‑198 (MQ=255)                                                                tCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAgc                                                                                                                                                      <  1:526085/232‑1 (MQ=255)                                                                  aaGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGATGAgga                                                                                                                                                                                                                           >  2:557717/1‑160 (MQ=255)                                                                            gATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGGGCAAGACCGTAGCGAGGGAACCCCGGa                                                                                                                                                             >  1:414083/1‑214 (MQ=255)                                                                                  aCTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAgcg                                                                                                                                                                                                     <  1:242946/167‑1 (MQ=255)                                                                                     cGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCg                                                                                                                                                                               >  1:672962/1‑186 (MQ=255)                                                                                                       gttgccgtGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAcctgcctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaagatcaag                                                                                                                                                            >  2:655523‑M1/1‑113 (MQ=255)                                                                                                             gtGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGgatga                                                                                                                                       >  2:692049/1‑202 (MQ=40)                                                                                                                       cgtCTAAGGGCGCGATAGTTCAGGGGACGCAGATTGCGTCTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGa                                                                                                                                                                                        <  2:232326/141‑1 (MQ=12)                                                                                                                                gcgcGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTg                                                                                                <  2:657692/222‑1 (MQ=38)                                                                                                                                 cgcgATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGTGAGGTGACGGGCATGTATGCCCGTCAAgcg                                                                                                                                                                                                                                  <  2:98454/91‑1 (MQ=16)                                                                                                                                    gATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAAcccc                                                                                                                                                                >  2:521775/1‑154 (MQ=35)                                                                                                                                                      cttcgCGACTTCAGGAGGCCGAACGCA‑GGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCttt                                                              >  2:287936/5‑234 (MQ=255)                                                                                                                                                               ttCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGAAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCt                                                      >  2:671293/1‑233 (MQ=255)                                                                                                                                                                tCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCTTCgg                                                  >  1:477110/1‑236 (MQ=39)                                                                                                                                                                    gaggCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCTTCGGTAGa                                              >  1:381364/1‑236 (MQ=39)                                                                                                                                                                            gCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTc                                                             <  1:478685/213‑1 (MQ=39)                                                                                                                                                                                  gggCTTGCGGAGGGCGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGgat                                                                                                                                         <  2:130684/132‑1 (MQ=17)                                                                                                                                                                                   ggCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCtt                                                               <  1:399460/205‑1 (MQ=38)                                                                                                                                                                                          ggagggagGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACccgccg                                                                              >  2:87291/1‑183 (MQ=32)                                                                                                                                                                                                       gggCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCTTCGGTAGAATTCGCTGAAATCCAGCCAGGAAGACCCGAGc              >  2:209308/1‑234 (MQ=37)                                                                                                                                                                                                           aTGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCTTCGGTAGAATTCGCTGAAATCCAGTCAGGAAGACCCGAGCGAtgtg        >  1:187236/1‑236 (MQ=37)                                                                                                                                                                                                               aTGCCCGTCAcctgcctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaacggcaacggcaacggcaacggcaacggcaacagcaacagcaacaacagcacagtacagttttgccttgtt                                                                     >  2:720475‑M1/1‑10 (MQ=255)                                                                                                                                                                                                                  cccGTCAcctgcctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaacggcaacggcaacggcaacggcaacggcaacagcaacagcaacaacagcacagtacagttttgccttgttatttccattgactgtactggccggtgactccgcataatgacgcctcgaattcacccggaggtggcat  <  2:525877‑M1/235‑229 (MQ=255)                                                                                                                                                                                                                     gTCAcctgcctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaacggcaacggcaacggcaacggcaacggcaacagcaacagcaacaacagcacagtacaggtttgccttgttatttccattgactgtactggccggtgactccgcataatgacgcctcgaattcaccc             >  1:88946‑M1/1‑4 (MQ=255)                                                                                                                                                                                                                      tCAcatgcctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaacggcaacggcaacggcaacggcaacggcaacagcaacagcaacaacagcacagtacagttttgccttgttatttccattgactgtactggccggtga                                          <  2:637992‑M1/191‑189 (MQ=255)                                                                                                                                                                                                                       cAcctgcctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaacggcaacggcaacggcaacggcaacggcaacagcaacagcaacaacagcacagtaca                                                                                  <  2:138690‑M1/150‑149 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     CTTTCTCCACCGAAGACCAGAAAGAAGGCATGGCCGCATTCATCGCCAAGCGTGAGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTA‑GGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCTTCGGTAGAATTCGCTGAAATCCAGTCAGGAAGACCCGAGCGATGTGGGCTGA  >  AE015451/2528797‑2529239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.