New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1594248	32 (0.800)	4 (0.110)	3/320	NT	11.0%	coding (1175/1320 nt)	kgtP	Alpha‑ketoglutarate permease
	?	AE015451	= 1594250	34 (0.890)					coding (1173/1320 nt)	kgtP	Alpha‑ketoglutarate permease

CATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAGGTCTGGATGGTGTGCAGGGTGCTGAGGATGGGGACGGTGAACAGGGTGCCGAGCACACCGAAGG  >  AE015451/1594063‑1594470                                                                                                                                                                                            |                                                                                                                                                                                                                             cATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAgg                                                                                                                                                                              >  1:569787/1‑236 (MQ=255)          gggCGCCCCGTTGAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCg                                                                                                                                                                                                           <  2:23801/198‑1 (MQ=255)                    tttAGTACTGCCAGGCGAGCGTTACTCAGTGGTGATCCGCGAGTGCTGCTTGGTGTCTTTGATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACAcc                                                                                                                                                                                                            <  1:264010/187‑1 (MQ=255)                     ttAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCg                                                                                                                                                                                                           <  2:456754/187‑1 (MQ=255)                                 ggccagCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCtt                                                                                                                                               >  1:488154/1‑235 (MQ=255)                                  gccagCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGG‑AGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGa                                                                                                                                            <  1:279535/236‑1 (MQ=255)                                        gTTACTCAGTTGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTGCCAGTAGAAGCCGCTCTCCATCCCGGCTCTCTTGAACCACAGGGCCACGTATTCGGCGGTGccgccgaatacgtggccctgtggttcaaga                                                                                                                                                                                                >  2:244003‑M2/1‑149 (MQ=255)                                                  ggTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGccgccgaatacgtggccctgtggttcaagagcgccgggatggagagcggcttcta                                                                                                                                                                       >  2:615933‑M2/1‑139 (MQ=255)                                                    tAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAAc                                                                                                                                                                                     >  1:662917/1‑178 (MQ=255)                                                           cgAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGc                                                                                                                    <  1:303604/236‑1 (MQ=255)                                                            gAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCa                                                                                                                                                                                       >  2:742999/1‑168 (MQ=255)                                                                      tGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGt                                                                                                                         <  1:164271/220‑1 (MQ=255)                                                                       ggTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGaa                                                                                                                                                                       <  2:444836/173‑1 (MQ=255)                                                                                 aTGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGc                                                                                              >  1:502783/1‑236 (MQ=255)                                                                                   ggTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGccgccgaatacgtggccctgtggttcaaga                                                                                                                                                                                                >  1:327440‑M2/1‑106 (MQ=255)                                                                                                          aGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCccac                                                                     >  1:423606/1‑236 (MQ=255)                                                                                                          aGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTAT‑CGGCGGTGcc                                                                                                                                                                                                                            >  1:353995‑M2/1‑82 (MQ=255)                                                                                                          aGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTAT‑CGGCGGTGcc                                                                                                                                                                                                                            <  2:353994‑M2/84‑3 (MQ=255)                                                                                                           gacGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCTACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCAccc                                                                        <  2:423605/230‑1 (MQ=255)                                                                                                                           aCGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCAccc                                                                        >  1:333569/1‑216 (MQ=255)                                                                                                                           aCGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCAccc                                                                        <  2:333568/216‑1 (MQ=255)                                                                                                                             gTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAg                                                                                                                                                                                           >  1:552696/1‑99 (MQ=255)                                                                                                                             gTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAg                                                                                                                                                                                           <  2:552695/99‑1 (MQ=255)                                                                                                                             gTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGcc                                                                                                                                                >  2:22786/1‑141 (MQ=255)                                                                                                                             gTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGga                                                                                   <  2:488153/203‑1 (MQ=255)                                                                                                                                  aGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAGGTCTGGATGGTGTGCAGGGt                                              >  1:725606/1‑235 (MQ=255)                                                                                                                                    tAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAGGTCTGGATGGTGTGCAGGGTGCt                                           <  1:388197/236‑1 (MQ=255)                                                                                                                                     aGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCACCGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTAc                                                                                                             >  1:21168/1‑169 (MQ=255)                                                                                                                                             ctctCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGccgccgaatacgtggccctgtggttcaagagcgccgggatgg                                                                                                                                                                                    >  2:608096‑M2/1‑48 (MQ=255)                                                                                                                                              tctcCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAg                                                                  >  2:563385/1‑203 (MQ=255)                                                                                                                                                       cGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAGGTCTGGATGGTGTGCAGGGTGCTGAGGATGGGGACGGTGa                          <  2:384121/234‑1 (MQ=255)                                                                                                                                                               ttGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCgg                                                                                               >  1:565303/1‑157 (MQ=255)                                                                                                                                                                     cacaGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAGGTCTGGATGGTGTGCAGGGt                                              >  1:94776/1‑200 (MQ=255)                                                                                                                                                                     cacaGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAGGTCTGGATGGTGTGCAGGGt                                              <  2:94776/200‑1 (MQ=255)                                                                                                                                                                          ggCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAgg                                                                                    >  1:222680/1‑157 (MQ=255)                                                                                                                                                                          ggCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAGGTCTGGATGGTGTGCAGGGTGCTGAGGATGGGGACGGTGAACAGGGTGCCGAGCACACCg      <  1:349260/235‑1 (MQ=255)                                                                                                                                                                            ccACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAGGTCTGGATGGTGTGCAGGGTGCTGAGGATGGGGACGGTGAACAGGGTg                  >  2:737117/1‑221 (MQ=255)                                                                                                                                                                                    tCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGtt                                                                                                                                  <  2:40917/101‑1 (MQ=255)                                                                                                                                                                                      ggcggTGCCGCCGAAGATCGACGCCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGgaaga                                                                                <  2:249051/149‑1 (MQ=255)                                                                                                                                                                                       gcggTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGtt                                                                                                                                  <  2:181282/98‑1 (MQ=255)                                                                                                                                                                                        cggTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGt                                                                                                                         <  2:685493/106‑1 (MQ=255)                                                                                                                                                                                         ggTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCAccc                                                                        <  2:390222/154‑1 (MQ=255)                                                                                                                                                                                         ggTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCAccc                                                                        >  1:390223/1‑154 (MQ=255)                                                                                                                                                                                            gccgccGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAGGTCTGGATGGTGTGCAGGGTGCTGAGGATGGGGACGGTGAACAGGGTGACGAGCACACCGAAgg  >  2:309198/1‑221 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                                                             CATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCACCCCACCAGGTCTGGATGGTGTGCAGGGTGCTGAGGATGGGGACGGTGAACAGGGTGCCGAGCACACCGAAGG  >  AE015451/1594063‑1594470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.