New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	3643464 =	32 (0.800)	3 (0.080)	3/316	NT	8.9%	coding (583/804 nt)	PP_3210	ABC transporter, permease protein
	?	AE015451	3643546 =	31 (0.820)					coding (501/804 nt)	PP_3210	ABC transporter, permease protein

CGCCACGCCAGTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTGCAGCAGGATCGCATAGGGAAAC  >  AE015451/3643312‑3643744                                                                                                                                                                                                                                           |                                                                                                                                                                                                       cgccacgccaGTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTg                                                                                                                                                                                                        <  1:323531/235‑1 (MQ=255)           gTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGtt                                                                                                                                                                                                <  2:718601/233‑1 (MQ=255)                              gggCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAg                                                                                                                                                                           <  1:570317/235‑1 (MQ=255)                                  ggcgAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAg                                                                                                                                                                      >  1:266340/1‑236 (MQ=255)                                      aaTAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCGCCCCGCCGATCAAGGCCAAGCCGATGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCCGGCGCGGTTCAGATGGaa                                                                                                                                                                                       >  2:706699/1‑215 (MQ=255)                                                        aGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCa                                                                                                                                                 <  1:385826/235‑1 (MQ=255)                                                        aGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGGTCAGATGGAACAGGTTGAGCAGGCCAgg                                                                                                                                                                     >  1:345309/1‑215 (MQ=255)                                                                              gcTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCg                                                                                                                            <  2:448116/233‑1 (MQ=255)                                                                                           gCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGccacc                                                                                                              >  1:486448/1‑235 (MQ=255)                                                                                            cccGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCTAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGc                                                                                                                  <  2:585637/230‑1 (MQ=255)                                                                                             ccGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCCGGCCCAGGACgg                                                                                                                                           >  2:496400/1‑204 (MQ=255)                                                                                                   ccGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGAt                                                                                                                                                                                           <  2:366692/150‑1 (MQ=255)                                                                                                   ccGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGAt                                                                                                                                                                                           >  1:366693/1‑150 (MQ=255)                                                                                                   ccGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGgc                                                                                                      >  1:747607/1‑235 (MQ=255)                                                                                                         cgccCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATc                                                                                               <  1:671604/236‑1 (MQ=255)                                                                                                                 ccGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAg                                                                                                                                                <  2:352833/179‑1 (MQ=255)                                                                                                                   ggCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGc                                                                                                           <  2:289127/214‑1 (MQ=255)                                                                                                                                  caccacCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTg                                                                       <  1:166410/235‑1 (MQ=255)                                                                                                                                            ccGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAgg                                                                                  <  2:22325/214‑1 (MQ=255)                                                                                                                                            ccGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAgg                                                                                  >  1:22325/1‑214 (MQ=255)                                                                                                                                             cGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCCGATGGAACAg                                                                                                                                                                                    >  2:162868/1‑115 (MQ=255)                                                                                                                                                  aaGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGtt                                                                                                                                                                                 <  1:235315/113‑1 (MQ=255)                                                                                                                                                     gCCAGGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCGCGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCa                                                                                     <  2:166373/202‑1 (MQ=255)                                                                                                                                                         AGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTgg                                                                                                                                     >  2:621364‑M1/1‑150 (MQ=255)                                                                                                                                                         AGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGAt                                                                                                >  2:49118‑M1/1‑187 (MQ=255)                                                                                                                                                          GCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATc                                                                                               >  2:368702‑M1/1‑187 (MQ=255)                                                                                                                                                            CGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGcat                                                                                                                                                      <  1:40765‑M1/130‑2 (MQ=255)                                                                                                                                                            CGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGTCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGAt                                             >  1:710030‑M1/1‑235 (MQ=255)                                                                                                                                                            CGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGGTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGGGGTGGCGATGATCGGGa                                                                                                                         <  2:109966‑M1/159‑1 (MQ=255)                                                                                                                                                                    aTGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGc                                                                                                                  <  2:340368/158‑1 (MQ=255)                                                                                                                                                                        ctgttgcgcctgcgcattcccagcgccttaccgtgctttttcgctggcctgcgcatcgccagcggctGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCgg                                 >  1:562622‑M2/68‑235 (MQ=255)                                                                                                                                                                              cAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTAc                           >  1:721364/1‑235 (MQ=255)                                                                                                                                                                                gCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCTCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTg                                                                                                                                                           <  2:735215/105‑1 (MQ=255)                                                                                                                                                                                    aaaaGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTgcagc                    <  1:542236/236‑1 (MQ=255)                                                                                                                                                                                     gcattcccagcgccttaccgtgcttttccgctggcctgcgcatcgccagcggctGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAg                                                                                                                                                      <  2:624390‑M2/51‑1 (MQ=255)                                                                                                                                                                                            ggTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTGCAGCAGGATCGc            <  1:228571/236‑1 (MQ=255)                                                                                                                                                                                                  gcgcTGGGAAGGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTGCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCGCCAACGCCAAGGTCGTGTCGCTGCACCAg                                                                 <  2:228287/177‑1 (MQ=255)                                                                                                                                                                                                           aTGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGAt                                                                                                                                                                                           >  2:22636/1‑46 (MQ=255)                                                                                                                                                                                                           aTGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGAt                                                                                                                                                                                           <  1:22636/46‑1 (MQ=255)                                                                                                                                                                                                                    tggcctgcgcatcgccagcggctGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTgcagca                   <  2:562621‑M2/182‑1 (MQ=255)                                                                                                                                                                                                                        aCAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTGCAGCAGGATCGCATAGGGAAAc  <  1:231870/218‑1 (MQ=255)                                                                                                                                                                                                                                    agcggctGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGccacca                                     >  1:605794‑M2/8‑171 (MQ=255)                                                                                                                                                                                                                                    agcggctGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGccacca                                     <  2:605793‑M2/164‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                       CGCCACGCCAGTCAGGGCGATCAACACCAGGGCGGCGAATAGGCGGGGGATGTTGAGCTGGAATCCCGCTTGCAGGATCTGATACGCCAGGCCCGCACCCGTGCCGCCCGTACCGGCGACGAACTCGGCCACCACCGCGCCGATCAAGGCCAAGCCGCTGGCGATGCGCAGGCCAGCGAAAAAGCACGGTAAGGCGCTGGGAATGCGCAGGCGCAACAGCACTTGCCAGCGGCTGGCGCGGTTCAGATGGAACAGGTTGAGCAGGCCAGGGTTGACGCTGCGCAGGCCCAGGACGGTGTTGGCGATGATCGGGAAGATCGCCACCAGCGTGGCGCAGATCACCAACGCCAAGGTCGTGTCGCTGCACCAGATGATGATCAGCGGCGCGATGGCCACCACCGGCGTTACCTGCAGCAGGATCGCATAGGGAAAC  >  AE015451/3643312‑3643744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.