New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	5783602 =	42 (1.050)	4 (0.100)	4/322	NT	10.0%	coding (2077/3309 nt)	kefA	mechanosensitive channel protein, intermediate (smaller, MscS‑like) conductance, K+ efflux
	?	AE015451	5783631 =	32 (0.840)					coding (2106/3309 nt)	kefA	mechanosensitive channel protein, intermediate (smaller, MscS‑like) conductance, K+ efflux

CGGTGGCCGAGCACCAGCCTTCGGCACTGGCAGACGACGTGTTGGGCATCGGCGTGGTGCTGACCTGCTATGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCTGGCCTACCAGCGTGCGTTGACCAAGCGTGCGGCGGCCAAGGAAGGGCTGGATGGCGAAGTCATCACCGAGGAGCCAACGCTG  >  AE015451/5783411‑5783817                                                                                                                                                                                                                             |                                                                                                                                                                                           cggtggcCGAGCACCAGCCTTCGGCACTGGCAGACGACGTGTTGGGCATCGGCGTGGTGCTGACCTGCTATGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTAcc                                                                                                                                                                                >  2:480177/1‑233 (MQ=255)                                      cgTGTTGGGCATCGGCGTTGTGCTGACCTGCTATGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGAt                                                                                                                                                                             <  1:4254/199‑1 (MQ=255)                                       agcccttccttggccgccgcacgcttggtcaacgcacgctggtaggccaggcgccgtgcagccaccgacaggccgcgcacgaaggcggcttcgatcaccaaccagaacagcagcaggtacagggtgtagatgagtcgatcggtaagcttcagtGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCAt                                                                                                                                                                     <  1:134333‑M1/53‑1 (MQ=255)                                         gtTGGGCATCGGCGTGGTGCTGACCTGCTATGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCtggttgg                                                                                                                                     >  1:631507/1‑236 (MQ=255)                                                   ggCGTGGTGCTGACCTGCTATGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAg                                                                                                                            <  1:344408/235‑1 (MQ=255)                                                               aCCTGCTATGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCt                                                                                                                                                                   <  1:17939/184‑1 (MQ=255)                                                               aCCTGCTATGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCt                                                                                                                                                                   >  2:17939/1‑184 (MQ=255)                                                                  tGCTATGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGCCACCTCGCTGTGCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTAcc                                                                                                                                                       <  1:136853/193‑1 (MQ=255)                                                                       tGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGAt                                                                                                                                 >  1:365579/1‑210 (MQ=255)                                                                       tGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTACGCAGGGCGGTGGGGCTAGCGTACACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGc                                                                                                           >  2:709653/1‑232 (MQ=255)                                                                            tAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCAt                                                                                                                                                                     >  1:223320/1‑169 (MQ=255)                                                                            tAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCAt                                                                                                                                                                     <  2:223320/169‑1 (MQ=255)                                                                            gctggtaggcccggcgccgtgcagccaccgacaggccgcgcacgaaggcggcttcgatcaccaaccagaacagcagcaggtacagggtgtagatgagtcgatcggtaagcttcagtGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTa                                                                                                                                                                                  >  2:134333‑M1/117‑156 (MQ=255)                                                                                  ccTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCAACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTc                                                                                                      <  2:385895/226‑1 (MQ=255)                                                                                     ggCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGt                                                                                                                                                          <  2:736126/171‑1 (MQ=255)                                                                                     ggCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACg                                                                                          <  1:480178/235‑1 (MQ=255)                                                                                         gctCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCGCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTAcc                                                                                                                                                       >  1:486057/1‑170 (MQ=255)                                                                                         gctCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTAcc                                                                                                                                                       <  2:486056/170‑1 (MQ=255)                                                                                         gctCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTg                                                                                              >  2:42992/1‑227 (MQ=255)                                                                                               ccGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCg                                                                                                                                                                           <  2:170777/144‑1 (MQ=255)                                                                                                   ctgctgCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCTGGCCTACCa                                                                           >  1:431868/1‑236 (MQ=255)                                                                                                            agcagcCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGgc                                                                                        <  1:377737/214‑1 (MQ=255)                                                                                                                  ccGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGAt                                                                                                                                                                             <  2:507084/123‑1 (MQ=255)                                                                                                                      cacaCCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGCCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTcgcgcgcgct                                                                                                           >  2:223547/1‑183 (MQ=255)                                                                                                                         acCGGGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCTGGCCTACCAGCGTGCGTTGACCAAGCGt                                                        <  2:270469/233‑1 (MQ=255)                                                                                                                              gACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGAt                                                                                                                                                                             >  2:354417/1‑111 (MQ=255)                                                                                                                              gACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGAt                                                                                                                                                                             <  1:354418/111‑1 (MQ=255)                                                                                                                                                                 gTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCTGGCCTACCCgcgtgcgct                                                                  >  1:215876/1‑181 (MQ=255)                                                                                                                                                                  ttCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCTGGCCTACCAGCGTGCGTTGACCAAGCGTGCGGCGGCCAAGGAAGGGCTGGATGGCGAAGTCATCACCgagg             <  1:437648/235‑1 (MQ=255)                                                                                                                                                                        atgagtcgatcggtaagcttcagtgccgtgtagtagtagccgaagcacacggcACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCTGGCCTACCAGCGTGCGTTGACCAAGCGTGCGGCGGCCAAGGAAGGGCt                                    <  1:134333‑M2/153‑1 (MQ=255)                                                                                                                                                                            tGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCTGGCCTACCAGCGTGCGTTGACCAAGCGTGCGGCGGCCAAGGAAGGGCTGGATGGCGAAGTCATCACCGAGGAGCCAACGCTg  >  1:393071/1‑236 (MQ=255)                                                                                                                                                                                 aTCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCAt                                                                                                                                                                     <  1:545966/68‑1 (MQ=255)                                                                                                                                                                                   cGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGgc                                                                                        <  2:549578/143‑1 (MQ=255)                                                                                                                                                                                     taagcttcagtgccgtgtagtagtagccgaagcacacggcACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCGGGCCTACCAgc                                                                         >  2:134333‑M2/41‑156 (MQ=255)                                                                                                                                                                                            cgtcgCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCTGGCCTACCAgcgtgcg                                                                    <  2:202189/154‑1 (MQ=255)                                                                                                                                                                                                            tagccgaagcacacggcACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGt                                                                                                                  <  2:313936‑M2/75‑1 (MQ=255)                                                                                                                                                                                                            tagccgaagcacacggcACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGt                                                                                                                  >  1:313937‑M2/18‑92 (MQ=255)                                                                                                                                                                                                              ctactactaCTCGGCACTGAAGCTTACCGATCGCCTCATCTCCACCCTGTTCCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCGTCGTGCGCGGCCGGTCGGTGGCTGCACGGCGCCTGGCCTACCAGCGTGCGTTGACCAAGCGTGCGGCGGCCAAGGAAGGGCTGGATGGCGAAGTCATCACCGAGGAGCCAAc      <  2:201763/198‑1 (MQ=255)                                                                                                                                                                                                                     tacACGGCACTGAAGCTTACCGATCGACTCATCTACCCCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCTGGCCTACCAGCGTGCGTTGACCAAGCGTGCGGCGGCCAAGGAAGGGCTGGAt                                <  2:284599/165‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                           CGGTGGCCGAGCACCAGCCTTCGGCACTGGCAGACGACGTGTTGGGCATCGGCGTGGTGCTGACCTGCTATGCGCTAATGGCCTGGCTGCTCAGCCGTCTGCTGCTCAGCAGCCCGGCACACCGTGACACCTCGCTGTTCCGCAGGGCGGTGGGGGTAGCGTTCACCGCCCTGCCCATCGCCCTGTTCGTCGCCGTGTGCTTCGGCTACTACTACACGGCACTGAAGCTTACCGATCGACTCATCTACACCCTGTACCTGCTGCTGTTCTGGTTGGTGATCGAAGCCGCCTTCGTGCGCGGCCTGTCGGTGGCTGCACGGCGCCTGGCCTACCAGCGTGCGTTGACCAAGCGTGCGGCGGCCAAGGAAGGGCTGGATGGCGAAGTCATCACCGAGGAGCCAACGCTG  >  AE015451/5783411‑5783817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.