New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451 = 520532063 (1.020)3 (0.050) 3/326 NT 3.9% coding (1113/1209 nt) PP_4586 conserved exported protein of unknown function
?AE015451 = 5205480 89 (1.520)coding (953/1209 nt) PP_4586 conserved exported protein of unknown function
Rejected: Frequency below/above cutoff threshold.

ATACCCGCTCAGGGCCCAAGTTCAAATAACAAGGCCGTGCGGCGAAGCTTGGGCTGTCAAAGGCCGGGACAGGCGCCAGGGATGCCTTGGGCCTGTATGAAAAGCCTTGAGACGAAGGTCAGGCAAGGCTTTTCATACGGGCCTTAGTGCAACACCTTGCTCTGCAGCACATCCGAACGCCGCCCCAGCACGTTCTCGCTGATCTGCACGAAGTCCTCGGTACTGACGCTGGGCAGGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5205081‑5205320
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcaggcgcGTCTGAGCAAGCAGGGCGCCAAGGTCAACCGGCTCAAGGGTGAGCGCGCAAGCCCGGCCCAGGTGGCCCGTGGCGTGATGCTGTTGCAGGCATTGGACAAGGAAATCCGCATTCACACCCAGGCCAAACGTTCGTTGGACGATGTAGTGCGTGGGCTGATGCGCCTGCCCAGCGTCAGTACCGAGGACTTCGTGCAGATCAGCGAGAACGTGCTGGGGCGGCGTTCGGATGTG  <  AE015451/5205480‑5205248
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
ATACCCGCTCAGGGCCCAAGTTCAAATAACAAGGCCGTGCGGCGAAGCTTGGGCTGTCAAAGGCCGGGACAGGCGCCAGGGATGCCTTGGGCCTGTATGAAAAGCCTTGAGACGAAGGTCAGGCAAGGCTTTTCATACGGGCCTTAGTGCAACACCTTGCTCTGCAGCACATCCGAACGCCGCCCCAGCACGTTCTCGCTGATCTGCACGAAGTCCTCGGTACTGACGCTGGGCAG                                                                                                                                                                                                                                               >  1:403264/1‑236
    CCGCTCAGGGCCCAAGTTCAAATAACAAGGCCGTGCGGCGAAGCTTGGGCTGTCAAAGGCCGGGACAGGCGCCAGGGATGCCTTGGGCCTGTATGAAAAGCCTTGAGACGAAGGTCAGGCAAGGCTTTTCATACGGGCCTTAGTGCAACACCTTGCTCTGCAGCACATCCGAACGCCGCCCCAGCACGTTCTCGCTGATCTGCACGAAGTCCTCGGTACTGACGCTGGGCAGGCGC                                                                                                                                                                                                                                           <  1:964967/236‑1
      GCTCAGGGCCCAAGTTCAAATAACAAGGCCGTGCGGCGAAGCTTGGGCTGTCAAAGGCCGGGACAGGCGCCAGGGATGCCTTGGGCCTGTATGAAAAGCCTTGAGACGAAGGTCAGGCAAGGCTTTTCATACGGGCCTTAGTGCAACACCTTGCTCTGCAGCACATCCGAACGCCGCCCCAGCACGTTCTCGCTGATCTGCACGAAGTCCTCGGTACTGACGCTGGGCAGGCGC                                                                                                                                                                                                                                           <  2:679715/234‑1
       CTCAGGGCCCAAGTTCAAATAACAAGGCCGTGCGGCGAAGCTTGGGCTGTCAAAGGCCGGGACAGGCGCCAGGGATGCCTTGGGCCTGTATGAAAAGCCTTGAGACGAAGGTCAGGCAAGGCTTTTCATACGGGCCTTAGTGCAACACCTTGCTCTGCAGCACATCCGAACGCCGCCCCAGCACGTTCTCGCTGATCTGCACGAAGTCCTCGGTACTGACGCTGGGCAGGCGC                                                                                                                                                                                                                                           <  2:727521/233‑1
                     aCAAATAACAAGGCCGCGCGGCGAAGCTTGGGCTGTCAAAGGCCGGGACCGGCGCCAGGGATGCCTTGGGCCTGTATGAAAAGCCTTGAGACGAAGGTCAGGCAAGGCTTTTCATACGGGCCTTAGTGCAACACCTTGCTCTGCAGCACATCCGAACGCCGCCCCAGCACGTTCTCGCTGATCTGCACGAAGTCCTCGGTACTGACGCTGGGCAGGC                                                                                                                                                                                                                                             <  1:319680/216‑1
                           AACAAGGCCGTGCGGCGAAGCTTGGGCTGTCAAAGGCCGGGACAGGCGCCAGGGATGCCTTGGGCCTGTATGAAAAGCCTTGAGACGAAGGTCAGGCAAGGCTTTTCATACGGGCCTTAGTGCAACACCTTGCTCTGCAGCACATCCGAACGCCGCCCCAGCACGTTCTCGCTGATCTGCACGAAGTCCTCGGTACTGCCGCTGGGCAGGCGCct                                                                                                                                                                                                                                         >  1:89906/1‑213
                                                                                                          TTGAGACGAAGGTCAGGCAAGGCTTTTCATACGGGCCTTAGTGCAACACCTTGCTCTGCAGCACATCCGAACGCCGCCCCAGCACGTTCTCGCTGATCTGCACGAAGTCCTCGGTACTGACGCTGGGCAGGCGCGTCTGAGCAAGCAGGGCGCCAAGGTCAACCGGCTCAAGGGTGAGCGCGCAAGCCCGGCCCAGGTGGC                                                                                                                                                                        <  2:819546/201‑1
                                                                                                                                                 AGTGCAACACCTTGCTCTGCAGCACATCCGAACGCCGCCCCAGCACGTTCTCGCTGATCTGCACGTAGTCCTCGGTACGGACGCTGGGCAGGCGCGTCTGAGCAAGCAGGGCGCCAAGGTCAACCGGCTCAAGGGTGAGCGCGCAAGCC                                                                                                                                                                                     <  2:908018/149‑1
                                                                                                                                                                                                     GCTGATCTGCACGAAGTCGTCGGTACTGACGCTGGGCAGGC                                                                                                                                                                                                                                             <  2:87001/41‑1
                                                                                                                                                                                                            TGCACGAAGTCCTCGGTACTGACGCTGGGCAGGCGCGTCTGAGCAAGCAG                                                                                                                                                                                                                             >  2:283113/1‑50
                                                                                                                                                                                                                                          AGGCGCGTCTGAGCAAGCAGGGCGCCAAGGTCAACCGCCTCAAGGGTGAGCGCGCAAGCCCGGCCCAGGTGGCCCGTGGCGTGATGCTGTTGCACGCATTGGACAAGGAAATCCGCATTCACACCCAGGCCAAA                                                                                                           >  2:308990/1‑134
                                                                                                                                                                                                                                               CGTCTGAGCAAGCAGGCCGCCAAGGTCAACCGGCTCAAGGGTGAGCGCGCAAGCCCGGCCCAGGTGGCCCGTGGCGTGATGCTGTTGCAGGCATTGGACAAGGAAATCCGCATTCACACCCAGGCCAAACGTTCGTTGGACGATGTAGTGCGTGGGCTGATGCGCCTGCCCAGCGTCAGTACCGAGGACTTCGTGCAGATCAGCGAGAACGTGCTGGGGCGGCGTTCGGATGTG  <  2:393347/234‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
ATACCCGCTCAGGGCCCAAGTTCAAATAACAAGGCCGTGCGGCGAAGCTTGGGCTGTCAAAGGCCGGGACAGGCGCCAGGGATGCCTTGGGCCTGTATGAAAAGCCTTGAGACGAAGGTCAGGCAAGGCTTTTCATACGGGCCTTAGTGCAACACCTTGCTCTGCAGCACATCCGAACGCCGCCCCAGCACGTTCTCGCTGATCTGCACGAAGTCCTCGGTACTGACGCTGGGCAGGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5205081‑5205320
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcaggcgcGTCTGAGCAAGCAGGGCGCCAAGGTCAACCGGCTCAAGGGTGAGCGCGCAAGCCCGGCCCAGGTGGCCCGTGGCGTGATGCTGTTGCAGGCATTGGACAAGGAAATCCGCATTCACACCCAGGCCAAACGTTCGTTGGACGATGTAGTGCGTGGGCTGATGCGCCTGCCCAGCGTCAGTACCGAGGACTTCGTGCAGATCAGCGAGAACGTGCTGGGGCGGCGTTCGGATGTG  <  AE015451/5205480‑5205248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.