Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	303,121	+GCT	89.4%	coding (1286/1314 nt)	envZ →	Osmolarity sensor protein EnvZ

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 303121	5 (0.090)	43 (0.710) +GCT	33/316	NT	89.4%	coding (1286/1314 nt)	envZ	Osmolarity sensor protein EnvZ
	?	AE015451	303122 =	5 (0.080)					coding (1287/1314 nt)	envZ	Osmolarity sensor protein EnvZ

GTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCG  >  AE015451/302914‑303319                                                                                                                                                                                                                |                                                                                                                                                                                                       gtgtGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGGCAGGTTGCCGCTGGGgctgctgct                                                                                                                                                                                               >  1:66249‑M1/1‑208 (MQ=255)   gtgCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgt                                                                                                                                                                           >  1:518163‑M1/1‑206 (MQ=255)                 cACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcc                                                                                                                                                             >  1:972324‑M1/1‑192 (MQ=255)                 cACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcc                                                                                                                                                             >  1:740413‑M1/1‑192 (MQ=255)                       tATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCCGGTTGCCGCTGGGgctg                                                                                                                                                                                                    >  2:8965‑M1/1‑186 (MQ=255)                           gACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctg                                                                                                                                                                                              <  2:607815‑M1/192‑11 (MQ=255)                                 tCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagc                                                                                                                                             <  1:721465‑M1/235‑60 (MQ=255)                                 gcgGGGCTGGAAACCATCTTCACCCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaa                                                                                                                                                <  2:560116‑M1/231‑57 (MQ=255)                                    gAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAAACGGGCTGGCGGCGGGATCGAGGCGCGGGTCAGGGTGCCGCTGGGgctgc                                                                                                                                                                                                   >  1:292600‑M1/1‑173 (MQ=255)                                          gAATCCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgc                                                                                                                                                                                          <  1:679386‑M1/181‑15 (MQ=255)                                              ccATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacag                                                                                                                                  <  2:972323‑M1/233‑71 (MQ=255)                                                  cTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgt                                                                                                                                                                             >  2:896033‑M1/1‑159 (MQ=255)                                                    tCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggatt                                                                                                                             <  2:679991‑M1/232‑76 (MQ=255)                                                         gcgTTCAGTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctg                                                                                                                                                                                              <  2:34921‑M1/161‑11 (MQ=255)                                                         ccGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGAACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgcc                                                                                                                                                                                         <  1:5245‑M1/167‑16 (MQ=255)                                                           gTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaa                                                                                                                                                <  1:362892‑M1/206‑57 (MQ=255)                                                                       tGATCGGGCACGGGGCGGCAAGGGCACCTGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGc                                                                                                                                                                         <  1:277835/169‑1 (MQ=255)                                                                                            gggCACCGGGCTGGGGCTGGTGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacag                                                                                                                                  <  1:495338‑M1/187‑71 (MQ=255)                                                                                            gggCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcggg                                                                                    <  2:740412‑M1/233‑117 (MQ=255)                                                                                                 ccGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTgc                                                                                                                              >  1:62614/1‑186 (MQ=255)                                                                                                   gggctggggctgGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaa                                                                                                                                                <  2:170216‑M1/166‑57 (MQ=255)                                                                                                        ggggatggCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgg                                                                                     <  1:368759‑M1/220‑116 (MQ=255)                                                                                                          ggctggCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGTGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagcc                                                                                                                                            <  2:1110690‑M1/163‑61 (MQ=255)                                                                                                             tggCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagc                                                                   <  2:643709‑M1/233‑134 (MQ=255)                                                                                                                 gATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacag                                                                                                                                  <  1:1124170‑M1/166‑71 (MQ=255)                                                                                                                 gATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacag                                                                                                                                  >  2:1124169‑M1/1‑96 (MQ=255)                                                                                                                      tCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacgg                                                                                                                                                    <  2:959086‑M1/143‑53 (MQ=255)                                                                                                                      tCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacgg                                                                                                                                                    >  1:959087‑M1/1‑91 (MQ=255)                                                                                                                        aaGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCAcc                                                                                                                                          <  2:62614/151‑1 (MQ=255)                                                                                                                            ggATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaag                                                                                                                                              <  2:853005‑M1/143‑59 (MQ=255)                                                                                                                                  ctgcGCAACATGGCGGGAACGGGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacaggga                                                                                                                               <  2:298846‑M1/152‑74 (MQ=255)                                                                                                                                        aaCATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtccgggtctgattgggcc                                     >  1:1101146‑M1/1‑73 (MQ=255)                                                                                                                                             ggCGGGAACGTGGAGTTGCGTATCCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaag                                                                                                                                              <  2:305208‑M1/126‑59 (MQ=255)                                                                                                                                             ggCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCCGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtcc                                                    >  1:1047428‑M1/1‑68 (MQ=255)                                                                                                                                             ggCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtccgggtctgattgggcccgc                                  <  2:1101145‑M1/234‑167 (MQ=255)                                                                                                                                                gggAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggccaaagccgcacctacagggattgcgca                                                                                                                        >  2:760996‑M1/1‑65 (MQ=255)                                                                                                                                                    ccgTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattg                                                                                                                            <  2:123648‑M1/136‑77 (MQ=255)                                                                                                                                                      gTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtccgggtctgattgggcccgccattc                             <  1:622035‑M1/230‑172 (MQ=255)                                                                                                                                                       tGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctccagggattgcgcagtacctgtggcagc                                                                                                          >  2:1096813‑M1/1‑58 (MQ=255)                                                                                                                                                              gCGTAACCGTTCGGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctc                                                                                                                                                           <  2:134991‑M1/96‑46 (MQ=255)                                                                                                                                                                  aaCCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtccgggtctgattgggcccgccattcttctccaggtgctcgat            >  1:1102419‑M1/1‑47 (MQ=255)                                                                                                                                                                         cTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCg     <  1:1086056/235‑1 (MQ=255)                                                                                                                                                                           ggcggcGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccg                                                                                                >  1:378346‑M1/1‑38 (MQ=255)                                                                                                                                                                            gcggggGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgcggcgcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggcc                                                                                                 <  2:513990‑M1/140‑104 (MQ=255)                                                                                                                                                                            gcggcgGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagagggttgaatcagcctttgccc                                                           >  1:27242‑M1/1‑37 (MQ=255)                                                                                                                                                                            gcggcgGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatccgcctttgcccttggtccgggtctgattgggcccgccattcttctccaggt                   >  1:1126411‑M1/1‑37 (MQ=255)                                                                                                                                                                                        gggcGCGGGTCCGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggccgcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttga                                                                         <  2:313638‑M1/151‑128 (MQ=255)                                                                                                                                                                                           cgcgGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCgggc                                                   >  1:60440/1‑170 (MQ=255)                                                                                                                                                                                                  cAGGTTGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcagtggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggca                                                                                                            >  2:924774‑M1/1‑15 (MQ=255)                                                                                                                                                                                                       tGCCGCTGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtccgggtctgattgggcccgccattcttctccaggtgctcgatgatcatcccg  <  1:725943‑M1/208‑199 (MQ=255)                                                                                                                                                                                                             tGGGgctgctgctgctgccgcgtaatgctgtgtgaggctcactggcctcttcacggctaaagccgcacctacagggattgcgcagtacctgtggcagcggcttggccgcgaagcggcgggtagaggcttgaatcagcctttgcccttggtccgggtct                                              >  2:1106435‑M1/1‑4 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                                                       GTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCGGCG  >  AE015451/302914‑303319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.