Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	303,121	+GCT	89.4%	coding (1286/1314 nt)	envZ →	Osmolarity sensor protein EnvZ

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 303121	5 (0.090)	43 (0.710) +GCT	33/316	NT	89.4%	coding (1286/1314 nt)	envZ	Osmolarity sensor protein EnvZ
	?	AE015451	303122 =	5 (0.080)					coding (1287/1314 nt)	envZ	Osmolarity sensor protein EnvZ

TCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCG  >  AE015451/302911‑303316                                                                                                                                                                                                                    |                                                                                                                                                                                                   gtgtgctggaccggggcaccggtattgacgagtcggagctggaaaccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcggggcaggttgccgctggggctgctgct                                                                                                                                                                                               >  1:66249‑M2/212‑217 (MQ=255)   gtgctggaccggggcaccggtattgacgagtcggagctggaaaccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCtgtgt                                                                                                                                                                           >  1:518163‑M2/210‑235 (MQ=255)                 caccggtattgacgagtcggagctggaaaccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGcc                                                                                                                                                             >  1:972324‑M2/196‑235 (MQ=255)                 caccggtattgacgagtcggagctggaaaccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGcc                                                                                                                                                             >  1:740413‑M2/196‑235 (MQ=255)                       tattgacgagtcggagctggaaaccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtccggttgccgctggggctg                                                                                                                                                                                                    >  2:8965‑M2/190‑190 (MQ=255)                           gacgagtcggagctggaaaccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctgctgctg                                                                                                                                                                                              <  2:607815‑M2/7‑1 (MQ=255)                                 tcggagctggaaaccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGc                                                                                                                                             <  1:721465‑M2/56‑1 (MQ=255)                                 gcggggctggaaaccatcttcaccccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTaa                                                                                                                                                <  2:560116‑M2/53‑1 (MQ=255)                                    gagctggaaaccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaacgggctggcggcgggatcgaggcgcgggtcagggtgccgctggggctgc                                                                                                                                                                                                   >  1:292600‑M2/177‑178 (MQ=255)                                          gaatccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctgctgctgctgc                                                                                                                                                                                          <  1:679386‑M2/11‑1 (MQ=255)                                              ccatcttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                  <  2:972323‑M2/67‑1 (MQ=255)                                                  cttcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCtgt                                                                                                                                                                             >  2:896033‑M2/163‑186 (MQ=255)                                                    tcaacccgttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGAtt                                                                                                                             <  2:679991‑M2/72‑1 (MQ=255)                                                         gcgttcagtcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctgctgctg                                                                                                                                                                                              <  2:34921‑M2/7‑1 (MQ=255)                                                         ccgttcattcgcggtgatcgggcacggggcggcaagggaaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGcc                                                                                                                                                                                         <  1:5245‑M2/12‑1 (MQ=255)                                                           gttcattcgcggtgatcgggcacggggcggcaagggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTaa                                                                                                                                                <  1:362892‑M2/53‑1 (MQ=255)                                                                          tGATCGGGCACGGGGCGGCAAGGGCACCTGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGc                                                                                                                                                                      <  1:277835/169‑1 (MQ=255)                                                                                            gggcaccgggctggggctggtgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                  <  1:495338‑M2/67‑1 (MQ=255)                                                                                            gggcaccgggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCggg                                                                                    <  2:740412‑M2/113‑1 (MQ=255)                                                                                                   gggctggggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTaa                                                                                                                                                <  2:170216‑M2/53‑1 (MQ=255)                                                                                                    ccGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTgc                                                                                                                           >  1:62614/1‑186 (MQ=255)                                                                                                        ggggatggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGcggcgg                                                                                     <  1:368759‑M2/112‑1 (MQ=255)                                                                                                          ggctggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgtgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGcc                                                                                                                                            <  2:1110690‑M2/57‑1 (MQ=255)                                                                                                             tggcgatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGc                                                                   <  2:643709‑M2/130‑1 (MQ=255)                                                                                                                 gatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                  <  1:1124170‑M2/67‑1 (MQ=255)                                                                                                                 gatcgtcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAg                                                                                                                                  >  2:1124169‑M2/100‑166 (MQ=255)                                                                                                                      tcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACgg                                                                                                                                                    <  2:959086‑M2/49‑1 (MQ=255)                                                                                                                      tcaagcggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACgg                                                                                                                                                    >  1:959087‑M2/95‑143 (MQ=255)                                                                                                                           aaGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCAcc                                                                                                                                       <  2:62614/151‑1 (MQ=255)                                                                                                                            ggattgctgcgcaacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAg                                                                                                                                              <  2:853005‑M2/55‑1 (MQ=255)                                                                                                                                  ctgcgcaacatggcgggaacggggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGa                                                                                                                               <  2:298846‑M2/70‑1 (MQ=255)                                                                                                                                        aacatggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGcc                                     >  1:1101146‑M2/77‑236 (MQ=255)                                                                                                                                             ggcgggaacgtggagttgcgtatccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAg                                                                                                                                              <  2:305208‑M2/55‑1 (MQ=255)                                                                                                                                             ggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtccggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTcc                                                    >  1:1047428‑M2/72‑216 (MQ=255)                                                                                                                                             ggcgggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGc                                  <  2:1101145‑M2/163‑1 (MQ=255)                                                                                                                                                gggaacgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCCAAAGCCGCACCTACAGGGATTGCGCa                                                                                                                        >  2:760996‑M2/69‑145 (MQ=255)                                                                                                                                                    ccgtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTg                                                                                                                            <  2:123648‑M2/73‑1 (MQ=255)                                                                                                                                                      gtggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCAttc                             <  1:622035‑M2/168‑1 (MQ=255)                                                                                                                                                       tggagttgcgtaaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTCCAGGGATTGCGCAGTACCTGTGGCAGc                                                                                                          >  2:1096813‑M2/62‑152 (MQ=255)                                                                                                                                                              gcgtaaccgttcgggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCctc                                                                                                                                                           <  2:134991‑M2/42‑1 (MQ=255)                                                                                                                                                                  aaccggtctggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCgat            >  1:1102419‑M2/51‑235 (MQ=255)                                                                                                                                                                           ggcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCcg                                                                                                >  1:378346‑M2/42‑142 (MQ=255)                                                                                                                                                                            gcggggggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGCGGCGCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGcc                                                                                                 <  2:513990‑M2/100‑1 (MQ=255)                                                                                                                                                                            gcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGGTTGAATCAGCCTTTGccc                                                           >  1:27242‑M2/41‑178 (MQ=255)                                                                                                                                                                            gcggcgggatcgaggcgcgggtcaggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCCGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGt                   >  1:1126411‑M2/41‑218 (MQ=255)                                                                                                                                                                            cTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCg  <  1:1086056/235‑1 (MQ=255)                                                                                                                                                                                        gggcgcgggtccggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCGCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGa                                                                         <  2:313638‑M2/124‑1 (MQ=255)                                                                                                                                                                                              cgcgGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCgggc                                                >  1:60440/1‑170 (MQ=255)                                                                                                                                                                                                  caggttgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCAGTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCa                                                                                                            >  2:924774‑M2/19‑107 (MQ=255)                                                                                                                                                                                                       tgccgctggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCg  <  1:725943‑M2/195‑1 (MQ=255)                                                                                                                                                                                                             tggggctGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCt                                              >  2:1106435‑M2/8‑158 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                   TCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTCCAGGTGCTCGATGATCATCCCG  >  AE015451/302911‑303316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.