New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	685602 =	31 (0.760)	3 (0.080)	3/328	NT	9.1%	coding (645/2400 nt)	cadA‑II	cadmium translocating P‑type ATPase
	?	AE015451	685617 =	30 (0.750)					coding (630/2400 nt)	cadA‑II	cadmium translocating P‑type ATPase

CAAGGCGCGTTCCGGGCGCAGGGCTTCGAGGGCTCGGATGGCACTGGCGGTCTGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAGCATGGGTAATACCAGCGGCAGGGCCAACAGCAAGGCTGCACCGACTGCCAGGCGCTCGTTGCGCAGGCGGCGCTGGA  >  AE015451/685374‑685804                                                                                                                                                                                                                                     |                                                                                                                                                                                                           cAAGGCCCGTTCCGGGCGCAGGGCTTCGAGGGCTCGGATGGCACTGGCGGTCTGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCcggcgccggcgc                                                                                                                                                                                                     <  1:706907/236‑1 (MQ=255)                gcgcAGGGCTTCGAGGGCTCGGATGGCACTGGCGGTCTGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAgg                                                                                                                                                                                      <  1:236452/236‑1 (MQ=255)                  gcAGGGCTTCGAGGGCTCGGATGGCACTGGCGGTCTGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGc                                                                                                                                                                                     >  1:359894/1‑235 (MQ=255)                      ggCTTCGAGGGCTCGGATGGCACTGGCGGTCTGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAgg                                                                                                                                                                                      <  2:359893/230‑1 (MQ=255)                       gCTTCGAGGGCTCGGATGGCACTGGCGGTCTGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAgg                                                                                                                                                                                      <  1:84198/229‑1 (MQ=255)                                                  gTCTGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTgccggcgccgg                                                                                                                                                                                                        >  2:718349/1‑184 (MQ=255)                                                                          aGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGGGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGGCCATGTTgccggcgc                                                                                                                                                                                                           >  1:87443/1‑157 (MQ=255)                                                                                  gCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCggg                                                                                                                                                                    <  1:210506/188‑1 (MQ=255)                                                                                                   cAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCcggcgccggcgc                                                                                                                                                                                                     >  2:436975/1‑138 (MQ=255)                                                                                                   cAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAg                                                                                                                                                                           >  2:535736/1‑164 (MQ=255)                                                                                                       ctggtattacccatgctggtacacccgttcggcttgcactggatgctacccgcctgggcgccgttcctgctggccacacccgtgcagttcattctcggtgcccgcttctacattgcagcctggaagGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCgaga                                                                                                                                                            >  1:512989‑M1/127‑175 (MQ=255)                                                                                                       caccacGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAg                                                                                                <  1:420066/235‑1 (MQ=255)                                                                                                                      agacaggTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTAcc                                                                                 <  1:631365/229‑1 (MQ=255)                                                                                                                                     cGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCa                                                                                                       >  1:24480/1‑198 (MQ=255)                                                                                                                                         gCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGAGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGcc                                                                                                                        <  1:234905/177‑1 (MQ=255)                                                                                                                                          ccATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACt                                                                                                                                                     >  2:631364/1‑147 (MQ=255)                                                                                                                                             tGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGACCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAGCATGGGTAATACCAGCGGCa                                                           <  1:405327/234‑1 (MQ=255)                                                                                                                                                                       tACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAgg                                                                                                                                                                                      >  1:239536/1‑85 (MQ=255)                                                                                                                                                                       tACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAgg                                                                                                                                                                                      <  2:239535/85‑1 (MQ=255)                                                                                                                                                                               cAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGACCt                                                                                                                                                     >  2:5195/1‑110 (MQ=255)                                                                                                                                                                                 cacacccgtgcagttcattctcggtgcccgcttctacattgcagcctggaagGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCa                                                                                <  1:595788‑M1/125‑1 (MQ=255)                                                                                                                                                                                 cacacccgtgcagttcattctcggtgcccgcttctacattgcagcctggaagGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCa                                                                                >  2:595787‑M1/53‑177 (MQ=255)                                                                                                                                                                                   ccctAACCCGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAGCATGGGTAATACCAGc                                                               <  2:245802/189‑1 (MQ=255)                                                                                                                                                                                    cATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAgg                                                                                                                                                                                      >  1:399626/1‑72 (MQ=255)                                                                                                                                                                                    cATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAgg                                                                                                                                                                                      <  2:399625/72‑1 (MQ=255)                                                                                                                                                                                    cATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTCCCAGGCTGCAATGTAGAAGCGGGCACCGGGAATGAACTGCCCGGGTGTGGCCAGCAGGAAc                                                                                                                              >  2:227075/1‑128 (MQ=255)                                                                                                                                                                                         ccAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCACACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAGCATGGGTAATACCAGCGGCAg                                                          >  2:311662/1‑191 (MQ=255)                                                                                                                                                                                               cTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGGAGCATCCAGGGCAAGc                                                                                               >  1:171685/1‑148 (MQ=255)                                                                                                                                                                                                  gTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGcc                                                                                              <  2:406164/146‑1 (MQ=255)                                                                                                                                                                                                  gTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGcc                                                                                              >  1:406165/1‑146 (MQ=255)                                                                                                                                                                                                     cccAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAGCATGGGTAATACCAGCGGCAGGGCCAACAGCAAGGCTGCACCGACt                                 >  1:79692/1‑204 (MQ=255)                                                                                                                                                                                                       caggctcaccaGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTAcc                                                                                 <  1:238179/148‑1 (MQ=255)                                                                                                                                                                                                                       tCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAGCATGGGTAATACCAGCGGCAGGGCCAACAGc                                                >  1:144445/1‑171 (MQ=255)                                                                                                                                                                                                                         cATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGGAGCAGCCAgg                                                                                                     >  2:215405/1‑115 (MQ=255)                                                                                                                                                                                                                          aTGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCgg                                                                                                                 <  2:215746/103‑1 (MQ=255)                                                                                                                                                                                                                              tGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGCACGGTTGTACCAGCCTggg                                                                         >  2:692889/1‑139 (MQ=255)                                                                                                                                                                                                                                   gcgccggcgcGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTAcc                                                                                 <  1:377673/126‑1 (MQ=255)                                                                                                                                                                                                                                     GCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAGCATGGGTAATACCAGCGGCAGGGCCAACAGCAAGGCTGCACCGACTGCCAGGCGCTCGTTGCGCAGGCGGCGCTGGa  <  2:323604‑M1/203‑1 (MQ=255)                                                                                                                                                                                                                                     GCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAGCATGGGTAATACCAGCGGCAGGGCCAACAGCAAGGCTGCACCGACTGCCAGGCGCTCGTTGCGCAGGCGGCGCTGGa  >  1:323605‑M1/1‑203 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                           CAAGGCGCGTTCCGGGCGCAGGGCTTCGAGGGCTCGGATGGCACTGGCGGTCTGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAGCATGGGTAATACCAGCGGCAGGGCCAACAGCAAGGCTGCACCGACTGCCAGGCGCTCGTTGCGCAGGCGGCGCTGGA  >  AE015451/685374‑685804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.