New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	3663643 =	22 (0.540)	4 (0.110)	4/304	NT	16.4%	coding (267/891 nt)	PP_3227	Transcriptional regulator, LysR family
	?	AE015451	3663659 =	21 (0.570)					coding (251/891 nt)	PP_3227	Transcriptional regulator, LysR family

GCGCGATCGACTGGCTGGCGATCCCTTCGGGGAAAATCTTGCCAGGCGGGAACAGGGCCGCGGCAGCGTCCAGCTCACCGTTTTCCATGCGCGCGATCAGGCTGCTGCCCCAGCCGCTGGTGACCTGGGTGCGCAGTTGGGGGTAGGCCTCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGCTTGAGCGGCTTGGTGTTGCGGTCGAGGAGGGTGGCGCCGAGGTCTTCCTCGAAATTCTGCACCCGGCGGGTAATGGCCGGCTGCGTCAGTTGCAGGGCCTCGGCGGCAAGGTTGGTGGACTGGCAACGA  >  AE015451/3663420‑3663871                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     tcgccgtcattcgttgccagtccaccaaccttgccgccgaggccctgcaactgacgcagccggccattacccgccgggtgcagaatttcgaggaagacctcggcgccaccctcctcgaccgcaacaccaagccgctcaagcccacccccatgggcttgcgggtctatgagcagtgcaaggcgatcctgcgcgagatagactcgctgcgtgaactggtagccaaCGACGgcgcgc                                                                                                                                                                                                                            >  2:544090‑M1/224‑234 (MQ=255)                        ccTTCGGGGAAAATCTTGCCAGGCGGGAACAGGGCCGCGGCAGCGTCCAGCTCACCGTTTTCCATGCGCGCGATCAGGCTGCTGCCCCAGCCGCTGGTGACCTGGGTGCGCAGTTGGGGGTAGGCCTCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACg                                                                                                                                                                                                      <  2:534274/233‑1 (MQ=255)                               ggAAAATCTTGCCAGGCGGGAACAGGGCCGCGGCAGCGTCCAGCTCACCGTTTTCCATGCGCGCGATCAGGCTGCTGCCCCAGCCGCTGGTGACCTGGGTGCGCAGTTGGGGGTAGGCCTCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCt                                                                                                                                                                                            >  1:275173/1‑236 (MQ=255)                                                                                               gATCAGGCTGCGGCCCCAGCCGCTGGTGGCCTGGGTGCGCAGTTGGGGGTAGGCCTCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACg                                                                                                                                                                                                      <  2:238118/162‑1 (MQ=255)                                                                                                 tCAGGCTGCTGCCCCAGCCGCTGGTGACCTGGGTGCGCAGTTGGGGGTAGGCCTCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACg                                                                                                                                                                                                      <  2:585928/160‑1 (MQ=255)                                                                                                    tcggcgccaccctcctcgaccgcaacaccaagccgcccaagcccccccccatgggcttgcgggtctatgagcagtgcaaggcgatcctgcgcgagatagactcgctgcgtgaactggtagccaaCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAg                                                                                                                                                                                                   >  2:382454‑M1/125‑160 (MQ=255)                                                                                                       tgctgcCCCAGCCGCTGGTGACCTGGGTGCGCAGTTGGGGGTAGGCCTCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGCTTGAGCGGCTTGgt                                                                                                                     >  1:168469/1‑235 (MQ=255)                                                                                                                aGCCGCTGGTGACCTGGGTGCGCAGTTGGGGGTAGGCCTCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCa                                                                                                                                                                                  <  2:305163/165‑1 (MQ=255)                                                                                                                             cTGGGTGCGCAGTTGGGGGTAGGCCTCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCtt                                                                                                                                                                        <  2:153418/162‑1 (MQ=255)                                                                                                                                   gcgcAGTTGGGGGTAGGCCTCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGCTTGAGCGGCTTGGTGTTGCGGTCGAGGAGGGTGGCGCCGAg                                                                                          >  1:386499/1‑234 (MQ=255)                                                                                                                                                      tCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCa                                                                                                                                                                                  <  2:647571/127‑1 (MQ=255)                                                                                                                                                      tCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCa                                                                                                                                                                                  >  1:647572/1‑127 (MQ=255)                                                                                                                                                      tCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATgggg                                                                                                                                         >  2:501709/1‑168 (MQ=255)                                                                                                                                                                 cgtctatgagccgtgcaagtcgatcctgcgcgagatagactcgccgcgtgaactggcagccaaAGACGGCGCGCCGTCGATGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCa                                                                                                                                                                                  >  1:680711‑M1/64‑116 (MQ=255)                                                                                                                                                                    cAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATgggg                                                                                                                                         >  1:130907/1‑154 (MQ=255)                                                                                                                                                                    cAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGCTTGAGCGGCTTGGTGTTGCGGTCGAGGAGGGTGGCGCCGAGGTCTTCCTCGAAATTCTGCACCCGGCGGGTAAt                                                         >  2:402757/1‑234 (MQ=255)                                                                                                                                                                    cAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGCGGGCTTGAGCGGCTTGGTGTTGCGGTCGAGGAGGGGGGCGCCGAGGTCTTCCTCg                                                                                >  1:69305/1‑211 (MQ=255)                                                                                                                                                                          gTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCcgtc                                                                                                                                                                                                                        <  2:459009/69‑1 (MQ=255)                                                                                                                                                                              agcagcACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCACGGGGGTGGGCTTGAGCGGCTTGGTGTTGCGGTCGAGGAGGGTGGCGCCGAGGTCTTc                                                                                    >  2:446408/1‑197 (MQ=255)                                                                                                                                                                                caaggcgatcctgcgcgagatagactcgctgcgtgaactggtagccaaCGACGgc                                                                                                                                                                                                                                >  2:672055‑M1/49‑55 (MQ=255)                                                                                                                                                                                caaggcgatcctgcgcgagatagactcgctgcgtgaactggtagccaaCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGCTCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGc                                                                                                                                   <  2:738717‑M1/100‑1 (MQ=255)                                                                                                                                                                                 ggcaCAACGTGGCGCAATGTCTGCGGCACACCCAGGCGCAAGGGGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCg                                                                                                                                                      <  2:23397/127‑1 (MQ=255)                                                                                                                                                                                      accgccGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGCtt                                                                                                                                 <  2:263192/139‑1 (MQ=255)                                                                                                                                                                                           cGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGCtt                                                                                                                                 >  2:393913/1‑139 (MQ=255)                                                                                                                                                                                                aaTGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCCTAGACCCGCAAGCCCATg                                                                                                                                            >  2:52504/1‑123 (MQ=255)                                                                                                                                                                                                 agatagactcgctgcgtgaactggtagccaaCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGGCTATCTCGCGCAGGATCGCCTTGCa                                                                                                                                                                     <  2:529666‑M1/66‑1 (MQ=255)                                                                                                                                                                                                                      gcAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGCTTGAGCGGCCTGGTGTTGCGGTCGAGGAGGGTGGCGCCGAGGTCTTCCTCGAAATTCTGCACCCGGCGGGTAATGGCCGGCTg                                                >  1:511880/1‑193 (MQ=255)                                                                                                                                                                                                                          ggTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGCTTGAGCGGCTTGGTGTTGCGGTCGAGGAGGGTGGCGCCGAGGTCTTCCTCGAAATTCTGCACCCGGCGGGTAATGGCCGGCTGCGTCAGTTGCAGGGCCTCGGCGGCAAGGTTGGTGGACTGGCAACGa  >  1:746818/1‑235 (MQ=255)                                                                                                                                                                                                                             gCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGCTTGAGCGGCTTGGTGTTGCGGTCGAGGAGGGTGGCGCCGAGGTCTTCCTCGAAATTCTGCACCCGGCGGGTAATGGCCGGCTGCgg                                             >  1:108406/1‑188 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     GCGCGATCGACTGGCTGGCGATCCCTTCGGGGAAAATCTTGCCAGGCGGGAACAGGGCCGCGGCAGCGTCCAGCTCACCGTTTTCCATGCGCGCGATCAGGCTGCTGCCCCAGCCGCTGGTGACCTGGGTGCGCAGTTGGGGGTAGGCCTCACGGATCTGTGCCAGGGCGTCGAGCAGCACAACGTCGCCCAATGTCTGCGGCACACCCAGGCGCAAGGTGCCCGACGGCGCGCCGTCGTTGGCTACCAGTTCACGCAGCGAGTCTATCTCGCGCAGGATCGCCTTGCACTGCTCATAGACCCGCAAGCCCATGGGGGTGGGCTTGAGCGGCTTGGTGTTGCGGTCGAGGAGGGTGGCGCCGAGGTCTTCCTCGAAATTCTGCACCCGGCGGGTAATGGCCGGCTGCGTCAGTTGCAGGGCCTCGGCGGCAAGGTTGGTGGACTGGCAACGA  >  AE015451/3663420‑3663871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.