New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 1891006	154 (1.110)	3 (0.020)	3/290	23.1	2.0%	intergenic (+1/‑183)	nudL/sdaA	putative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase/L‑serine dehydratase 1
	?	CP009273	= 3633344	145 (1.070)					intergenic (‑264/‑127)	uspB/uspA	universal stress (ethanol tolerance) protein B/universal stress global response regulator
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTACATCTGGGTCGTTATCACCCTTTAGATATCTACCGCCGTGGTGATTCACATCGGGTATGGCTGTCCTGGTACGAACAGTATTTTGTATGGGGAATGACCGCAGGCATAATTCGTGAGCTGGCGCTGCAAATTGGTGTGAAACCCTGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/1890856‑1891006 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gacCATCCTTACCTTATAGCGACCCGGATGATCCGTCAATCCGCCTTGCTTACTCGTTAGATAAACAAATTTTCGACATGAATATATTGATAGTGGTTAACCTTCTGGAAAAAAAACAACCTGATCTCCTACACTATCTATAGAGCCGCTCG  <  CP009273/3633344‑3633196                                                                                                                                                                                                                                                                                                              TTACATCTGGGTCGTTATCACCCTTTAGATATCTACCGCCGTGGTGATTCACATCGGGTATGGCTGTCCTGGTACGAACAGTATTTTGTATGGGGAATGACCGCAGGCATAATTCGTGAGCTGGCGCTGCAAATTGGTGTGAAACCCTGA                                                                                                                                                        <  1:523061/150‑1  TACATCTGGGTCGTTATCACCCTTTAGATATCTACCGCCGTGGTGATTCACATCGGGTATGGCTGTCCTGGTACGAACAGTATTTTGTATGGGGAATGACCGCAGGCATAATTCGTGAGCTGGCGCTGCAAATTGGTGTGAAACCCTGAC                                                                                                                                                       >  1:1463957/1‑150   ACATCTGGGTCGTTATCACCCTTTAGATATCTACCGCCGTGGTGATTCACATCGGGTATGGCTGTCCTGGTACGAACAGTATTTTGTATGGGGAATGACCGCAGGCATAATTCGTGAGCTGGCGCTGCAAATTGGTGTGAAACCCTGAC                                                                                                                                                       <  1:1589360/149‑1                                                     ATCGGGTATGGCTGTCCTGGTACGAACAGTATTTTGTATGGGGAATGACCGCAGGCATAATTCGTGAGCTGGCGCTGCAAATTGGTGTGAAACCCTGACCATCCTTACCTTATAGCGACCCGGATGATCCGTCAATCCGCCTTGCTTACT                                                                                                    >  1:206208/1‑150                                                          GTATGGCTGTCCTGGTACGAACAGTATTTTGTATGGGGAATGACCGCAGGCATAATTCGTGAGCTGGCGCTGCAAATTGGTGTGAAACCCTGACCATCCTTACCTTATAGCGACCCGGATGATCCGTCAATCCGCCTTGCTTACTCGTTA                                                                                               <  2:206208/150‑1                                                                                                                                                    TGACCATCCTTACCTTATAGCGACCCGGATGATCCGTCAATCCGCCTTGCTTACTCGTTAGATAAACAAATTTTCGACATGAATATATTGATAGTGGTTAAGCTTCTGGAAAAAAAACAACCTGATCCCCTACACTATCTATAGAGCCGC     >  1:1859325/1‑150                                                                                                                                                     GACCATCCTTACCTTATAGCGACCCGGATGATCCGTCAATCCGCCTTGCTTACTCGTTAGATAAACAAATTTTCGACATGAATATATTGATAGTGGTTAACCTTCTGGAAAAAAAACAACCTGATCTCCTACACTATCTATAGAGCCGCT    >  1:1105152/1‑150                                                                                                                                                       CCATCCTTACCTTATAGCGACCCGGATGATCCGTCAATCCGCCTTGCTTACTCGTTAGATAAACAAATTTTCGACATGAATATATTGATAGTGGTTAACCTTCTGGAAAAAAAACAACCTGATCTCCTACACTATCTATAGAGCCGCTCG  >  1:400560/1‑150                                                                                                                                                                                                                                                                                                              TTACATCTGGGTCGTTATCACCCTTTAGATATCTACCGCCGTGGTGATTCACATCGGGTATGGCTGTCCTGGTACGAACAGTATTTTGTATGGGGAATGACCGCAGGCATAATTCGTGAGCTGGCGCTGCAAATTGGTGTGAAACCCTGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/1890856‑1891006 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gacCATCCTTACCTTATAGCGACCCGGATGATCCGTCAATCCGCCTTGCTTACTCGTTAGATAAACAAATTTTCGACATGAATATATTGATAGTGGTTAACCTTCTGGAAAAAAAACAACCTGATCTCCTACACTATCTATAGAGCCGCTCG  <  CP009273/3633344‑3633196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.