| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| JC | CP009273 | 374,639 | +GCCC | 100% | coding (389/1110 nt) | frmA ← | alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | CP009273 | = 374639 | 0 (0.000) | 51 (0.910) +GCCC |
43/258 | NT | 100% | coding (389/1110 nt) | frmA | alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase |
| ? | CP009273 | 374640 = | 0 (0.000) | coding (388/1110 nt) | frmA | alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase | |||||
GTGTACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCA > CP009273/374491‑374788 | gtgtACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCt < 2:955907/149‑1 (MQ=255)gtgtACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTg > 1:904957‑M1/1‑149 (MQ=255) tgtACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgc > 2:714435‑M1/1‑148 (MQ=255) tACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgccc > 1:208830‑M1/1‑146 (MQ=255) tACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgccc > 2:48583‑M1/1‑146 (MQ=255) ccGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgc < 1:469432‑M1/150‑7 (MQ=255) cgcCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccg > 1:866699‑M1/1‑141 (MQ=255) aTACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgt > 2:965888‑M1/1‑136 (MQ=255) ccGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaaga < 1:342099‑M1/149‑17 (MQ=255) aCGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggt < 1:479518‑M1/150‑27 (MQ=255) aCGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtg < 2:880208‑M1/150‑27 (MQ=255) cacaGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgc < 2:952949‑M1/150‑31 (MQ=255) aGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccg > 2:718842‑M1/1‑117 (MQ=255) cccAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtct < 2:718781‑M1/150‑36 (MQ=255) cagcagGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctg > 2:231057‑M1/1‑113 (MQ=255) aggcagACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatc < 1:401458‑M1/150‑42 (MQ=255) ttCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttacc > 1:391837‑M1/1‑98 (MQ=255) aTGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccc < 2:221591‑M1/149‑55 (MQ=255) tGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgg < 1:405400‑M1/150‑57 (MQ=255) ttCTGGATTAATTTTTGCCAGAGTCACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATATAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgc > 2:126656‑M1/1‑86 (MQ=255) ttCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgc > 2:147378‑M1/1‑86 (MQ=255) ttCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgc > 2:805110‑M1/1‑86 (MQ=255) gATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaacc > 2:654997‑M1/1‑81 (MQ=255) aaTTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaa > 2:701094‑M1/1‑77 (MQ=255) aaTTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaa > 2:755617‑M1/1‑77 (MQ=255) aaTTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaa > 2:415382‑M1/1‑77 (MQ=255) ttGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacag < 2:102075‑M1/149‑78 (MQ=255) tGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagag > 1:959947‑M1/1‑71 (MQ=255) gagaCACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggtta < 2:490826‑M1/150‑85 (MQ=255) agaCACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagt > 2:759229‑M1/1‑65 (MQ=255) gaCACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagt > 1:766987‑M1/1‑64 (MQ=255) gaCACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagt > 2:653803‑M1/1‑64 (MQ=255) cacTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagtttt < 1:805110‑M1/150‑89 (MQ=255) acTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagtttt < 2:246956‑M1/150‑90 (MQ=255) acTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttg > 1:976522‑M1/1‑61 (MQ=255) ttCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgc > 2:983699‑M1/1‑59 (MQ=255) ccGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgcca < 2:97764‑M1/149‑93 (MQ=255) cgcgACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccag < 1:714435‑M1/149‑94 (MQ=255) gACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaac < 1:890655‑M1/150‑98 (MQ=255) ggTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacag > 1:576550‑M1/1‑47 (MQ=255) ggTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaa > 2:671983‑M1/1‑47 (MQ=255) ttCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactca > 1:101797‑M1/1‑41 (MQ=255) ttCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcac < 2:208830‑M1/149‑109 (MQ=255) cACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacac > 2:129864‑M1/1‑39 (MQ=255) cACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacac > 2:750085‑M1/1‑39 (MQ=255) aaTGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgc < 2:866699‑M1/148‑115 (MQ=255) tAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcaaactcgccgcac < 1:965888‑M1/150‑121 (MQ=255) gagCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactcc < 1:415814‑M1/150‑123 (MQ=255) gCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgc > 2:520462‑M1/1‑26 (MQ=255) cACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgc > 2:214183‑M1/1‑25 (MQ=255) aCCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcg > 1:676200‑M1/1‑24 (MQ=255) aCCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcg < 2:904957‑M1/150‑127 (MQ=255) ccccATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggt < 1:643596‑M1/150‑128 (MQ=255) gTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcg > 2:812397‑M1/1‑14 (MQ=255) gTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcg > 1:122623‑M1/1‑14 (MQ=255) gATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcggga > 1:813000‑M1/1‑12 (MQ=255) tAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatc < 1:415382‑M1/150‑141 (MQ=255) aaGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcac < 1:48583‑M1/150‑143 (MQ=255) aaGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcac < 2:976522‑M1/150‑143 (MQ=255) aaGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatca > 1:930013‑M1/1‑8 (MQ=255) gCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcaca < 2:743749‑M1/150‑145 (MQ=255) gCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcaca < 2:101797‑M1/150‑145 (MQ=255) cGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcacatg > 1:259242‑M1/1‑5 (MQ=255) tgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcacatggtc > 1:583459‑M1/1‑1 (MQ=255) | GTGTACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCA > CP009273/374491‑374788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |