| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| JC | CP009273 | 374,639 | +GCCC | 100% | coding (389/1110 nt) | frmA ← | alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | CP009273 | = 374639 | 1 (0.020) | 43 (0.740) +GCCC |
40/258 | NT | 97.8% | coding (389/1110 nt) | frmA | alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase |
| ? | CP009273 | 374640 = | 1 (0.020) | coding (388/1110 nt) | frmA | alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase | |||||
GTGTACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCC > CP009273/374491‑374787 | gtgtACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCt < 1:731370/149‑1 (MQ=255) tACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgccc < 2:246206‑M1/150‑5 (MQ=255) tACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgccc < 1:40954‑M1/150‑5 (MQ=255) aCCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccg > 1:662303‑M1/1‑145 (MQ=255) cctcgccaaTACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgc < 2:5179‑M1/147‑7 (MQ=255) aaTACTGGAGGTCACGCCACAGCCCAGCAGGCCGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCCCTGTATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccggtg > 2:924723‑M1/1‑137 (MQ=255) aTACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgta < 2:653825‑M1/150‑15 (MQ=255) aCCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgt > 1:831947‑M1/1‑134 (MQ=255) gtggtCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaa < 2:770801‑M1/150‑21 (MQ=255) tggtCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaac > 1:1035675‑M1/1‑129 (MQ=255) ccACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggt < 1:758038‑M1/150‑30 (MQ=255) cacaGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtg > 1:832095‑M1/1‑120 (MQ=255) caGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgcc < 2:256732‑M1/150‑33 (MQ=255) cccAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCCCTGAATGTAGAGCCCCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaccgggtggtgccgtc < 1:391695‑M1/150‑36 (MQ=255) ccAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtct < 2:937337‑M1/150‑37 (MQ=255) aggcagACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcat < 1:398452‑M1/150‑42 (MQ=255) cagACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCt > 1:999164/1‑150 (MQ=255) gtgtTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctt < 1:392404‑M1/150‑50 (MQ=255) gtgtTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggccttt < 1:358993‑M1/150‑50 (MQ=255) tgtTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctt > 1:236939‑M1/1‑100 (MQ=255) tgtTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctt > 1:961049‑M1/1‑100 (MQ=255) ttCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttac < 1:328240‑M1/150‑53 (MQ=255) gTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgg < 2:1020613‑M1/149‑58 (MQ=255) ttGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtt > 1:265391‑M1/1‑90 (MQ=255) tGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggttt > 1:839160‑M1/1‑89 (MQ=255) cTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttc < 2:109109‑M1/149‑63 (MQ=255) gATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaacc > 1:533347‑M1/1‑81 (MQ=255) tAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgc > 1:494718‑M1/1‑78 (MQ=255) gCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacaga < 2:662303‑M1/149‑80 (MQ=255) gagaCACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggtta < 2:102355‑M1/150‑85 (MQ=255) agaCACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttag > 1:540421‑M1/1‑65 (MQ=255) acacTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttt > 2:619083‑M1/1‑63 (MQ=255) ccGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgc > 1:172327‑M1/1‑57 (MQ=255) aCGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacga > 1:187484‑M1/1‑49 (MQ=255) cGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgac < 1:436346‑M1/148‑101 (MQ=255) ggTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacag > 2:46050‑M1/1‑47 (MQ=255) gtgtATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaa > 2:847280‑M1/1‑46 (MQ=255) tATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaact > 1:7397‑M1/1‑43 (MQ=255) aTTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactca > 1:219783‑M1/1‑42 (MQ=255) aCTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacac < 2:831947‑M1/149‑112 (MQ=255) gAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgc > 2:367918‑M1/1‑35 (MQ=255) gAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgc > 1:877478‑M1/1‑35 (MQ=255) aaTGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcg < 2:274594‑M1/148‑115 (MQ=255) cACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgc > 1:115936‑M1/1‑25 (MQ=255) ccATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtg > 1:454625‑M1/1‑21 (MQ=255) aTGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgta < 2:265391‑M1/150‑132 (MQ=255) aTAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcggga < 2:532925‑M1/150‑140 (MQ=255) aaaGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggat > 1:122873‑M1/1‑9 (MQ=255) ggCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcacatg > 2:503589‑M1/1‑4 (MQ=255) ggCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcacatg < 1:367918‑M1/150‑147 (MQ=255) gCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcacatgg > 1:855831‑M1/1‑3 (MQ=255) gCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcacatgg > 1:765788‑M1/1‑3 (MQ=255) cTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcacatggt > 2:352693‑M1/1‑2 (MQ=255) | GTGTACCGCGCCAATACCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCC > CP009273/374491‑374787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |