Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	374,639	+GCCC	100%	coding (389/1110 nt)	frmA ←	alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 374639	0 (0.000)	6 (0.590) +GCCC	8/258	NT	100%	coding (389/1110 nt)	frmA	alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase
	?	CP009273	374640 =	0 (0.000)					coding (388/1110 nt)	frmA	alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase

ACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGT  >  CP009273/374537‑374783                                                                                                       |                                                                                                                                                 aCGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcc                                                                                                   <  1:155260‑M1/150‑48 (MQ=255)               tGCTTCTGGATTAATTTTTTCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTCGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggttt                                                                                     >  1:87943‑M1/1‑89 (MQ=255)                                                    aCTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacg                                                <  2:87943‑M1/150‑99 (MQ=255)                                                        cGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgaca                                             <  2:33510‑M1/149‑102 (MQ=255)                                                               ttCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactca                                      <  2:174050‑M1/149‑109 (MQ=255)                                                                      aaTGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcg                                <  2:192924‑M1/148‑115 (MQ=255)                                                                                                aaGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatca    >  2:23485‑M1/1‑8 (MQ=255)                                                                                                  gCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcaca  >  1:81914‑M1/1‑6 (MQ=255)                                                                                                       |                                                                                                                                                 ACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGT  >  CP009273/374537‑374783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.