Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	374,639	+GCCC	100%	coding (389/1110 nt)	frmA ←	alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 374639	0 (0.000)	6 (0.590) +GCCC	8/258	NT	100%	coding (389/1110 nt)	frmA	alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase
	?	CP009273	374640 =	0 (0.000)					coding (388/1110 nt)	frmA	alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase

GCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACA  >  CP009273/374533‑374779                                                                                                            |                                                                                                                                            acgtgttcatggtttgcttctggattaatttttgccagagacacttccgcgactacggtgtattcactgaatgtagagcaccccatgtagtgataaagcggctgcccGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGcc                                                                                                   <  1:155260‑M2/43‑1 (MQ=255)               tgcttctggattaattttttccagagacacttccgcgactacggtgtattcactgaatgtagagcaccccatgtcgtgataaagcggctgcccGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGttt                                                                                     >  1:87943‑M2/94‑150 (MQ=255)                                                    actacggtgtattcactgaatgtagagcaccccatgtagtgataaagcggctgcccGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACg                                                <  2:87943‑M2/94‑1 (MQ=255)                                                        cggtgtattcactgaatgtagagcaccccatgtagtgataaagcggctgcccGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACa                                             <  2:33510‑M2/97‑1 (MQ=255)                                                               ttcactgaatgtagagcaccccatgtagtgataaagcggctgcccGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTca                                      <  2:174050‑M2/104‑1 (MQ=255)                                                                      aatgtagagcaccccatgtagtgataaagcggctgcccGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTcg                                <  2:192924‑M2/110‑1 (MQ=255)                                                                                                aagcggctgcccGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATca    >  2:23485‑M2/13‑150 (MQ=255)                                                                                                  gcggctgcccGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATcaca  >  1:81914‑M2/11‑150 (MQ=255)                                                                                                            |                                                                                                                                            GCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACA  >  CP009273/374533‑374779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.