BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	CP009273	4,303,167	Δ450 bp	coding (1‑450/450 nt)	rpiB →	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP009273	4303167	4303616	450	133 [0]	[0] 134	rpiB	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 4303166	0 (0.000)	132 (0.880)	108/290	0.2	100%	intergenic (‑358/‑1)	alsR/rpiB	d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
*	?	CP009273	4303617 =	0 (0.000)	132 (0.880)	108/290	0.2	100%	intergenic (+1/‑68)	rpiB/yjdP	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase/putative periplasmic protein

Only 100 of 134 total aligned reads displayed.

ACACCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACA  >  CP009273/4303468‑4303766

tggtgagtgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgag                                                                                                                                                       <  2:441221‑M2/1‑1 (MQ=255)

     agtgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCa                                                                                                                                                  <  1:1800333‑M2/6‑1 (MQ=255)

       tgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATc                                                                                                                                                <  1:914098‑M2/8‑1 (MQ=255)

         tctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATcc                                                                                                                                               <  2:410458‑M2/9‑1 (MQ=255)

              aacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCactact                                                                                                                                         >  2:1551786‑M2/136‑150 (MQ=255)

              aacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCactac                                                                                                                                          <  1:528886‑M2/14‑1 (MQ=255)

               acgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACtt                                                                                                                                        <  1:753136‑M2/16‑1 (MQ=255)

               acgctattgcgctgtcagatgtaactctgtaaaacagatcagcaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttctttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACtt                                                                                                                                        <  2:1881104‑M2/16‑1 (MQ=255)

                cgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTg                                                                                                                                       <  1:2406902‑M2/17‑1 (MQ=255)

                  ctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCa                                                                                                                                     >  2:684700‑M2/132‑150 (MQ=255)

                   tattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTg                                                                                                                                       >  2:2332701‑M2/131‑147 (MQ=255)

                    attgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGc                                                                                                                                      <  1:2332701‑M2/18‑1 (MQ=255)

                    attgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATg                                                                                                                                   >  2:1100135‑M2/130‑150 (MQ=255)

                       gcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGAt                                                                                                                                >  1:1982469‑M2/127‑150 (MQ=255)

                        cgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGAt                                                                                                                                <  1:519299‑M2/24‑1 (MQ=255)

                        cgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGAt                                                                                                                                <  2:1464882‑M2/24‑1 (MQ=255)

                          ctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAg                                                                                                                             >  2:1140929‑M2/124‑150 (MQ=255)

                               agatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAAt                                                                                                                         <  1:785017‑M2/31‑1 (MQ=255)

                                  tgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGCTTCCTCCACTACTTGCATGGATGAGTAATGAtt                                                                                                                     <  2:1507638‑M2/35‑1 (MQ=255)

                                  tgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGAt                                                                                                                      >  2:1317734‑M2/116‑149 (MQ=255)

                                    taactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTaa                                                                                                                   <  2:2251363‑M2/37‑1 (MQ=255)

                                    taactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTa                                                                                                                    <  2:1778086‑M2/36‑1 (MQ=255)

                                       ctctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAAtgt                                                                                                                >  1:1566434‑M2/111‑150 (MQ=255)

                                       ctctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAAtgt                                                                                                                <  1:1231326‑M2/40‑1 (MQ=255)

                                          tgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAAtgtg                                                                                                               <  1:34803‑M2/41‑1 (MQ=255)

                                            taaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGa                                                                                                             >  1:80048‑M2/106‑148 (MQ=255)

                                            taaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGa                                                                                                             >  1:1028530‑M2/106‑148 (MQ=255)

                                            taaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGa                                                                                                             >  1:1324698‑M2/106‑148 (MQ=255)

                                               aacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATagag                                                                                                        >  2:71061‑M2/103‑150 (MQ=255)

                                                acagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGt                                                                                                       >  1:2156422‑M2/102‑150 (MQ=255)

                                                 cagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGtt                                                                                                      >  1:604276‑M2/101‑150 (MQ=255)

                                                    atcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCt                                                                                                   <  1:62697‑M2/53‑1 (MQ=255)

                                                    atcaggaaggcgtaattcatcgattttgcgtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCt                                                                                                   >  2:2019100‑M2/98‑150 (MQ=255)

                                                            ggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAgg                                                                                           >  1:804296‑M2/90‑150 (MQ=255)

                                                             gcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGt                                                                                          <  2:686058‑M2/62‑1 (MQ=255)

                                                                taattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTgg                                                                                       <  2:894255‑M2/65‑1 (MQ=255)

                                                                taattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTgg                                                                                       <  1:2394183‑M2/65‑1 (MQ=255)

                                                                taattcatagattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTgg                                                                                       >  2:2003612‑M2/86‑150 (MQ=255)

                                                                      atcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATg                                                                                 <  2:98190‑M2/71‑1 (MQ=255)

                                                                       tcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATg                                                                                 >  2:467836‑M2/79‑149 (MQ=255)

                                                                        cgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGa                                                                                >  2:624794‑M2/78‑149 (MQ=255)

                                                                          attttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAAc                                                                             >  2:1166877‑M2/76‑150 (MQ=255)

                                                                          attttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAAc                                                                             <  1:963345‑M2/75‑1 (MQ=255)

                                                                              tgggtgtttttagttcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCt                                                                          <  2:1995838‑M2/78‑1 (MQ=255)

                                                                                ggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCttt                                                                        <  1:1620334‑M2/80‑1 (MQ=255)

                                                                                  tgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCg                                                                     <  2:1390829‑M2/83‑1 (MQ=255)

                                                                                  tgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCg                                                                     >  1:393049‑M2/68‑150 (MQ=255)

                                                                                    tttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGc                                                                    <  2:2396682‑M2/84‑1 (MQ=255)

                                                                                      tttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTa                                                                  <  1:624794‑M2/86‑1 (MQ=255)

                                                                                       ttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAtt                                                                >  1:1250364‑M2/63‑150 (MQ=255)

                                                                                       ttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAtt                                                                >  2:2387313‑M2/63‑150 (MQ=255)

                                                                                        taattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAttt                                                               >  1:1503470‑M2/62‑150 (MQ=255)

                                                                                        taattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAttt                                                               >  2:1769696‑M2/62‑150 (MQ=255)

                                                                                        taattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAttt                                                               >  2:1658786‑M2/62‑150 (MQ=255)

                                                                                        taattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAtt                                                                <  1:921144‑M2/88‑1 (MQ=255)

                                                                                         aattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAttt                                                               <  2:1583130‑M2/89‑1 (MQ=255)

                                                                                               ttaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAt                                                        >  2:1975350‑M2/55‑150 (MQ=255)

                                                                                                taacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAtt                                                       >  2:2249275‑M2/54‑150 (MQ=255)

                                                                                                 aacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGCGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAtt                                                       >  2:1930287‑M2/53‑149 (MQ=255)

                                                                                                  acatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCa                                                     >  1:1287461‑M2/52‑150 (MQ=255)

                                                                                                    atcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCAcc                                                   >  2:1513791‑M2/50‑150 (MQ=255)

                                                                                                    atcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCAcc                                                   >  1:735635‑M2/50‑150 (MQ=255)

                                                                                                     tcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCAccc                                                  <  2:1372237‑M2/102‑1 (MQ=255)

                                                                                                       acaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCt                                                 <  2:1011227‑M2/103‑1 (MQ=255)

                                                                                                          aatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCATCCTGCTc                                             <  2:1947407‑M2/107‑1 (MQ=255)

                                                                                                          aatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCt                                              >  1:1194442‑M2/44‑149 (MQ=255)

                                                                                                          aatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCt                                              >  2:1326760‑M2/44‑149 (MQ=255)

                                                                                                           atgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCa                                            >  1:906502‑M2/43‑150 (MQ=255)

                                                                                                            tgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCAc                                           <  1:1085743‑M2/109‑1 (MQ=255)

                                                                                                            tgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCAc                                           <  2:966798‑M2/109‑1 (MQ=255)

                                                                                                              ttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACg                                          <  1:544996‑M2/110‑1 (MQ=255)

                                                                                                                ttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCt                                        <  2:80048‑M2/112‑1 (MQ=255)

                                                                                                                ttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCt                                        <  1:1059403‑M2/112‑1 (MQ=255)

                                                                                                                ttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTg                                       >  2:1322327‑M2/38‑150 (MQ=255)

                                                                                                                 tttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGt                                      >  1:1832942‑M2/37‑150 (MQ=255)

                                                                                                                   tgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTc                                     >  1:763440‑M2/35‑149 (MQ=255)

                                                                                                                    gattgtgaagttgtgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCGCCCTGCTCACGCTGTcc                                    <  2:1993529‑M2/116‑1 (MQ=255)

                                                                                                                     attgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCAc                                  <  2:471539‑M2/118‑1 (MQ=255)

                                                                                                                     attgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCAc                                  >  2:2036313‑M2/33‑150 (MQ=255)

                                                                                                                      ttgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCAcc                                 <  2:1270578‑M2/119‑1 (MQ=255)

                                                                                                                        gtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGt                               >  2:316578‑M2/30‑150 (MQ=255)

                                                                                                                         tgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGtt                              >  2:1044531‑M2/29‑150 (MQ=255)

                                                                                                                          gaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTc                             >  1:206583‑M2/28‑150 (MQ=255)

                                                                                                                            agttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCg                           <  2:1028530‑M2/125‑1 (MQ=255)

                                                                                                                             gttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCgg                          >  2:583108‑M2/25‑150 (MQ=255)

                                                                                                                              ttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGc                         <  2:306842‑M2/127‑1 (MQ=255)

                                                                                                                                  gcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAg                     >  1:1977247‑M2/20‑150 (MQ=255)

                                                                                                                                   cacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCaga                    >  2:933369‑M2/19‑149 (MQ=255)

                                                                                                                                   cacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAg                     <  1:996852‑M2/131‑1 (MQ=255)

                                                                                                                                      ggacggggaagatgaGATTCATCCACTACTTGCAGGGATGAGTAATGATTAATGCGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTGTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGcc                  >  1:2111887‑M2/16‑149 (MQ=255)

                                                                                                                                        acggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGat               <  2:374890‑M2/137‑1 (MQ=255)

                                                                                                                                          ggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGatat             >  2:63920‑M2/12‑150 (MQ=255)

                                                                                                                                           gggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatt            <  1:443246‑M2/140‑1 (MQ=255)

                                                                                                                                              aagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATc         <  1:1140929‑M2/143‑1 (MQ=255)

                                                                                                                                               agatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcg        <  1:1551786‑M2/144‑1 (MQ=255)

                                                                                                                                                 atgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgac      <  2:610351‑M2/146‑1 (MQ=255)

                                                                                                                                                 atgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgac      <  2:393049‑M2/146‑1 (MQ=255)

                                                                                                                                                 atgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgac      <  2:2087499‑M2/146‑1 (MQ=255)

                                                                                                                                                 atgaGATTCATCAACTACTTTAATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgac      <  1:1756093‑M2/146‑1 (MQ=255)

                                                                                                                                                  tgaGATTCTTCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgacg     <  2:2156422‑M2/147‑1 (MQ=255)

ACACCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACA  >  CP009273/4303468‑4303766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`