New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 3756498 =349 (1.160)4 (0.010) 3/234 NT 1.2% coding (130/678 nt) EO946_RS19340 CidB/LrgB family autolysis modulator
?NZ_CP034943 = 3756494 335 (1.200)coding (126/678 nt) EO946_RS19340 CidB/LrgB family autolysis modulator
Rejected: Frequency below/above cutoff threshold.

TCAAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGCAGCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/3756645‑3756498
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcagcagACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCCGATGAACACGTATGTTTTTT  <  NZ_CP034943/3756494‑3756358
                                                                                                                                                                                                                                                                                             
TCAAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACC                                                                                                                                                   <  2:1551215/140‑1
TCAAAATAATTTCCACTGAGTATTTCTTTAAAACACGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGGACGGGACATTCACC                                                                                                                                                   <  2:1540894/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATCTGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  1:2971202/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  1:161604/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:3668839/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:3082403/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:2456137/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:1584185/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:1539321/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:3860506/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  1:2971726/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:4309844/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  1:1747738/140‑1
  AAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCACCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  1:824248/140‑1
  AAAATAATTTCCACTGCGTATTACATTAAAACAGCGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:4398745/140‑1
  AAAATAATGTCCACGGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:3758760/140‑1
  AAAATAAGTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:3289946/140‑1
   AAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGC                                                                                                                                                <  1:4414066/140‑1
   AAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGC                                                                                                                                                <  1:1953363/140‑1
      TAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGCAGC                                                                                                                                             <  2:4169747/140‑1
       AATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGCAGCA                                                                                                                                            >  2:3307945/1‑140
       AATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGCAGCA                                                                                                                                            >  2:3295387/1‑140
        ATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGCAGCAG                                                                                                                                           >  2:481046/1‑140
                                              ATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 <  2:896674/96‑1
                                              ATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAG                                                                                                                                                 >  1:896674/1‑96
                                                                                TGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGCAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTT                                                                   <  1:243226/140‑1
                                                                                TGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGCAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTT                                                                   <  1:243193/140‑1
                                                                                  AAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGCAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTG                                                                 <  1:1245676/140‑1
                                                                                            GTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGCAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATA                                                       >  2:3729948/1‑140
                                                                                                                                           CAGCAGCATACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGAAACGCGGAAAGCGTTGATATACCTTTTTTGCGCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCAGATGAACACGTATG        >  2:492517/1‑140
                                                                                                                                           CAGCAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCAGATGAACACTTATG        >  2:4761843/1‑140
                                                                                                                                              CAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCCGATTAACACGTATGTTT     >  2:621566/1‑140
                                                                                                                                              CAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCCGATGAACACGTATGTTT     >  2:3302148/1‑140
                                                                                                                                              CAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCCGATGAACACGTATGTTT     >  2:3302379/1‑140
                                                                                                                                              CAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCCGATGAACACGTATGTTT     >  2:3109519/1‑140
                                                                                                                                              CAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCCGATGAACACGTATGTTT     >  1:2639497/1‑140
                                                                                                                                              CAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCCGATGAACACGTATGTTT     >  1:2638859/1‑140
                                                                                                                                              CAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCCGATGAACACGTATGTTT     >  2:4396044/1‑140
                                                                                                                                              CAGCAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTCTTAAAAACAAACTTACAATTCCGATGAACACGTATGTTT     >  2:3109777/1‑140
                                                                                                                                                 CAGACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCCGATGAACACGTATGTTTTTT  >  2:1836671/1‑140
                                                                                                                                                                                                                                                                                             
TCAAAATAATTTCCACTGCGTATTTCTTTAAAACAGGGAAATATTTATAAAGCGGGATCGCGAATGCCACGGTGGCCGGCTGAAGCATATCGGTCAGCAATCCGCCGCCGAGATTATATGACTCGTACGGGACATTCACCAGCAGCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/3756645‑3756498
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcagcagACCGACTAACACAGCGGGCGTCACCAATAGCGGCGACGTATAGACACGCGGAAAGCGTTGATATACCTTTTTTGCTCCTATATACACCAAAACTGTTAAAAACAAACTTACAATTCCGATGAACACGTATGTTTTTT  <  NZ_CP034943/3756494‑3756358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.