New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 1222275 =266 (1.820)3 (0.020) 3/242 NT 1.2% coding (52/159 nt) yjcZ sporulation protein YjcZ
?NZ_CP034943 = 1222271 255 (1.820)coding (48/159 nt) yjcZ sporulation protein YjcZ
Rejected: Frequency below/above cutoff threshold.

TACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/1222416‑1222275
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccgccATAACAGCCGCCGCCGTAGCCGCCTCCGAATCCGTATCCAAAGCCCATAGTTAAGCACCTCCTTTACTCTCTATACACTATGTGTGCAGCCTTAATCGGCTTGGATATTCGCATAGGGCGTTTGCGGATTTATGATTGT  <  NZ_CP034943/1222271‑1222133
                                                                                                                                                                                                                                                                                         
TACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCG                                                                                                                                               >  1:1091685/1‑140
TACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCG                                                                                                                                               >  1:1809402/1‑140
TACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCG                                                                                                                                               >  1:972876/1‑140
TACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAAACATAGCCGCCGCCGTAACCGCCAGCATAACCG                                                                                                                                               >  1:885677/1‑140
 agagtACATCACCCGTACTTACCTAAATAAACCTTAGAAGTTATACAAAGAAGCACCCACGATGATAAGGAGAATGAACAATACTACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGC                                                                                                                                              <  2:1365544/135‑1
 ACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGC                                                                                                                                              <  2:2234866/140‑1
 ACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGC                                                                                                                                              <  2:2158706/140‑1
 ACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGC                                                                                                                                              <  2:1265265/140‑1
 ACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGC                                                                                                                                              <  2:1265131/140‑1
 ACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGC                                                                                                                                              <  2:1228383/140‑1
 ACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCAGAGCCGCCGCCGTAACCGCCAGCATAACCGC                                                                                                                                              <  2:1154460/140‑1
  CAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGCC                                                                                                                                             >  1:83196/1‑140
  CAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGCC                                                                                                                                             >  1:784134/1‑140
  CAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGCC                                                                                                                                             >  2:1587783/1‑140
  CAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGCC                                                                                                                                             >  2:601557/1‑140
                                                                              atAATACGACCAACAAAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGCCATAACAGCCGCCGCCGTAGCCGCCTCCGAATCCGTATCCAAAGCCCATAGTTAAGCACCTCCTTTACTCTCTATAC                                                                 <  2:1715649/138‑1
                                                                                                         AACCATAGCCGCCGCCGTAACCGCCAGCATAACCGCCATAACAGCCGCCGCCGTAGCCGCCGCCGAATCCGTATCCAAAGCCCAT                                                                                             >  1:680070/1‑85
                                                                                                         AACCATAGCCGCCGCCGTAACCGCCAGCATAACCGCCATAACAGCCGCCGCCGTAGCCGCCGCCGAATCCGTATCCAAAGCCCAT                                                                                             <  2:680070/85‑1
                                                                                                                                          CGCCATAACAGCCGCCGCCGTAGCCGCCTCCGAATCCGTATCCAAAGCCCATAGTTAAGCACCTCCTTTACTCTCTATACACTATGTGTGCAGCCTTAATCGGCTTGGATATTCGCATAGGGCGTTTGCGGATTTATGAT     <  2:2171383/140‑1
                                                                                                                                             CATAACAGCCGCCGCCGTAGCCGCCTCCGAATCCGTATCCAAAGCCCATAGTTAAGCACCTCCTTTACTCTCTATACACTATGTGTGCAGCCTTAATCGGCTTGGATATTCGCATAGGGCGTTTGCGGATTTATGATTGT  <  1:99621/140‑1
                                                                                                                                             CATAACAGCCGCCGCCGTAGCCGCCTCCGAATCCGTATCCAAAGCCCATAGTTAAGCACCTCCTTTACTCTCTATACACTATGTGTGCAGCCTTAATCGGCTTGGATATTCGCATAGGGCGTTTGCGGATTTATGATTGT  <  1:102878/140‑1
                                                                                                                                             CATAACAGCCGCCGCCGTAGCCGCCTCCGAATCCGTATCCAAAGCCCATAGTTAAGCACCTCCTTTACTCTCTATACACTATGTGTGCAGCCTTAATCGGCTTGGATATTCGCATAGGGCGTTTGCGGATTTATGATTGT  <  1:1605084/140‑1
                                                                                                                                             CATAACAGCCGCCGCCGTAGCCGCCTCCGAATCCGTATCCAAAGCCCATAGTTAAGCACCTCCTTTACTCTCTATACACTATGTGTGCAGCCTTAATCGGCTTGGATATTCGCATAGGGCGTTTGCGGATTTATGATTGT  <  1:1464300/140‑1
                                                                                                                                             CATAACAGCCGCCGCCGTAGCCGCCTCCGAATCCGTATCCAAAGCCCATAGTTAAGCACCTCCTTTACTCTCTATACACTATGTGTGCAGCCTTAATCGGCTTGGATATTCGCATAGGGCGTTTGCGGATTTATGATTGT  <  1:1177308/140‑1
                                                                                                                                                                                                                                                                                         
TACAGGACATCACCCGTACTTCCCTCAATAAACCTTAGAAGTTATAAAAAGATGCACCCACGATGATAAGGAGAATGAACAATACGACTAAAAGAACAAATGTTGAACCATAGCCGCCGCCGTAACCGCCAGCATAACCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/1222416‑1222275
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccgccATAACAGCCGCCGCCGTAGCCGCCTCCGAATCCGTATCCAAAGCCCATAGTTAAGCACCTCCTTTACTCTCTATACACTATGTGTGCAGCCTTAATCGGCTTGGATATTCGCATAGGGCGTTTGCGGATTTATGATTGT  <  NZ_CP034943/1222271‑1222133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.