New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 908850 =347 (0.770)3 (0.010) 3/242 NT 0.88% coding (151/258 nt) sspE gamma type acid‑soluble spore protein SspE
?NZ_CP034943 = 908846 340 (0.780)coding (147/258 nt) sspE gamma type acid‑soluble spore protein SspE
Rejected: Frequency below/above cutoff threshold.

TTGTTTTGTTCAGCAGATTGGTTTTGCTGTCTTACCTGCTGTACGTCAGTTTCACTAGCGAATTCAGTGCCGAATTGACCTTGTTGGCCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/908941‑908850
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGACCAGCAGCTGATTGTTGGTTTTGTTTTCTAACTTGTTGTGCGTTTGTTTTGCTGAAATTGTTTGA  <  NZ_CP034943/908846‑908709
                                                                                                                                                                                                                                      
TTGTTTTGTTCAGCAGATTGGTTTTGCTGTCTTACCTGCTGTGCGTCAGTTTCACTAGCGAATTCAGTGCCGAATTGACCTTGTTGTCCAGCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGC                                                                                            <  2:6393030/140‑1
                               TTACCTGCTGTGCGTCAGTTTCACTAGCGAATTCAGTGCCGAATTGACCTTGTTGTCCAGCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCAAATTGACCTTGACCAGCA                                                             >  2:6706546/1‑140
                                           CGTCAGTTTCACTAGCGAATTCAGTGCCGAATTGACCTTGTTGTCCAGCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTGTGTTTCGCTAGCAAATTCAGTGCCAAATTGACCTTGACCAGCAGCTGATTGTTGG                                                 >  1:2951273/1‑140
                                                                                        CAGCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGTCGAGCAGCGGATTGTTGGTTTTGTGTTGTACCTTGTTGTGCGTTTGTTGTGCTGAAATTGTTg    >  2:4745129/1‑139
                                                                                        CAGCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGACCAGCAGCTGATTGTTGGTTTTGTTTTCTAACTTGTTGTGCGTTTGTTTTGCTGAAATTGTTT    >  1:3465583/1‑140
                                                                                        CAGCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGACCAGCAGCTGATTGTTGGTTTTGTTTTCTAACTTGTTGTGCGTTTGTTTTGCTGAAATTGTTT    >  2:2946163/1‑140
                                                                                        CAGCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGACCAGCAGCTGATTGTTGGTTTTGTTTTCTAACTTGTTGTGCGTTTGTTTTGCTGAAATTGTTT    >  2:3989992/1‑140
                                                                                        CAGCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGACCAGCAGCTGATTGTTGGTTTTGTTTTCTAACTTGTTGTGCGTTTGTTTTGCTGAAATTGTTT    >  2:4311136/1‑140
                                                                                        CAGCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGACCAGCAGCTGATTGTTGGTTTTGTTTTCTAACTTGTTGTGCGTTTGTTTTGCTGAAATTGTTT    >  2:4340274/1‑140
                                                                                        CAGCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGACCAGCAGCTGATTGTTGGTTTTGTTTTCTAACTTGTTGTGCGTTTGTTTTGCTGAAATTGTTT    >  2:7349174/1‑140
                                                                                          GCTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGACCAGCAGCTGATTGTTGGTTTTGTTTTCTAACTTGTTGTGCGTTTGTTTTGCTGAAATTGTTTGA  <  2:648535/140‑1
                                                                                          GCTGATTGCTGGTTTTGTTGTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGACCAGCAGCTGATTGTTGGTTTTGTTTTCTAACTTGTTGTGCGTTTGTTTTGCTGAAATTGTTTGA  <  2:3001239/140‑1
                                                                                                                                                                                                                                      
TTGTTTTGTTCAGCAGATTGGTTTTGCTGTCTTACCTGCTGTACGTCAGTTTCACTAGCGAATTCAGTGCCGAATTGACCTTGTTGGCCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/908941‑908850
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcTGATTGCTGGTTTTGTTTTCTGACTTGCTGAGCGTTTGTTTCGCTAGCAAATTCAGTGCCGAATTGACCTTGACCAGCAGCTGATTGTTGGTTTTGTTTTCTAACTTGTTGTGCGTTTGTTTTGCTGAAATTGTTTGA  <  NZ_CP034943/908846‑908709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.