New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 607643239 (0.780)7 (0.020) 7/254 10.0 1.9% coding (486/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607824 = 480 (1.650)coding (667/1125 nt) EO946_RS03185 collagen‑like protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/607500‑607643
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agccggaCCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCA  >  NZ_CP034943/607824‑607962
                                                                                                                                                                                                                                                                                           
GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGC                                                                                                                                                 >  1:2953557/1‑140
   GCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGG                                                                                                                                              <  1:4237095/140‑1
   GCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGG                                                                                                                                              <  1:3826914/140‑1
   GCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGG                                                                                                                                              <  1:6091/140‑1
   GCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCTGAGCGACAGGAGCTACCCGGGACGCCGGACCAGCCGG                                                                                                                                              >  1:4508413/1‑140
     TGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGgg                                                                                                                                            >  1:250746/1‑138
         CCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAGCCGcagagcc                                                                                                                                        >  2:3414494/1‑133
                                                                                             GCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCA                                                                                                                                                   >  1:2537200/1‑45
                                                                                             GCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCA                                                                                                                                                   <  2:2537200/45‑1
                                                                                             GCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCA                                                                                                                                                   >  1:1678923/1‑45
                                                                                                                      CTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCAC                                                                                                                      >  1:2181034/1‑49
                                                                                                                      CTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCAC                                                                                                                      <  2:2181034/49‑1
                                                                                                                         CCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGT                                                                          >  1:1946276/1‑90
                                                                                                                         CCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGT                                                                          <  2:1946276/90‑1
                                                                                                                          CGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGT                                                                                         >  2:3888365/1‑74
                                                                                                                          CGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGT                                                                                         <  1:3888365/74‑1
                                                                                                                                   cggcgCAGCCGGACCACCCGGAGCGACCGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAA              <  1:3704427/135‑1
                                                                                                                                          GCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGAT       >  1:4382136/1‑140
                                                                                                                                           CCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATA      <  2:4457732/140‑1
                                                                                                                                            CGGACCAACCGGAGCGACAGGAGCGACGGGAGCCACAGGCACAGGAGAAGCTGGGTTATCCGAATCCGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAA     >  2:687750/1‑140
                                                                                                                                            CGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAA     >  2:1693933/1‑140
                                                                                                                                             GGACCACCCGGAGCGACAGGAGCGACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGTTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGTCTGATGTCATTTTTGATTCAACAGGGATAAC    <  1:3748643/140‑1
                                                                                                                                             GGACCAACCGGAGCGACAGGAGCGACAGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAAC    <  2:2304025/140‑1
                                                                                                                                             GGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGGAGCTGTGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAAC    <  1:2784185/140‑1
                                                                                                                                             GGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAAC    <  1:378320/140‑1
                                                                                                                                             GGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAAC    <  1:4693664/140‑1
                                                                                                                                             GGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAAC    <  2:238011/140‑1
                                                                                                                                             GGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAAC    <  1:2780665/140‑1
                                                                                                                                             GGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAAC    <  2:4327898/140‑1
                                                                                                                                             GGACCAACCGGAGCGACAGGAGACACGGGGGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAAC    <  1:331683/140‑1
                                                                                                                                               ACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATCACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCA  >  1:3151669/1‑140
                                                                                                                                               ACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCA  >  1:3380516/1‑140
                                                                                                                                               ACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCA  >  1:2790529/1‑140
                                                                                                                                               ACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCA  >  1:1553035/1‑140
                                                                                                                                               ACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCA  >  1:1217794/1‑140
                                                                                                                                                                                                                                                                                           
GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/607500‑607643
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agccggaCCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCA  >  NZ_CP034943/607824‑607962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.