New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 2526375 =283 (0.900)3 (0.010) 3/252 NT 0.85% intergenic (‑96/‑100) nucB/sigK sporulation‑specific Dnase NucB/RNA polymerase sporulation sigma factor SigK
?NZ_CP034943 3751590 = 415 (1.320)coding (64/600 nt) EO946_RS19305 DsbA family protein
Rejected: Frequency below/above cutoff threshold.

CAAAGCCGAGCGCTGCGAAAACACCTGTCACCATCGTCACCTCCACAAAAGTATGTAGGCAAAAGCCTATATGTAAATGTATGTGAGCGGGAGGCTGTCTGTGTCTGTACCGGGAAATTTCTCGTAGGAGAGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/2526508‑2526375
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCTTTTCCAATGAAACAAAATGGGCAGACGTAATCTGAATAAACCTTGATGTGTACTGTCATGATAATCTCCTTTCGTATAAGTTGATTTTAAACTGTAACAATTAAAGTTACAAATGTTTTTTTAAAAAGAAG  >  NZ_CP034943/3751590‑3751725
                                                                                                                                                                                                                                                                              
CAAAGCCGAGCGCTGCGAAAACACCTGTCACCATCGTCACCTCCACAAAAGTATGTAGGCAAAAGCCTATATGTAAATGTATGTGACCGGGAGGCTGTCTGTGTCTGTACCGGGAAATTTCTCGCGGGGGAGTCCGGCTT                                                                                                                                    >  2:387571/1‑140
                                                                                          GAGGCTGTCTGTGTCTGTACCGGGAAATTTCTCGCGGGGGAGTCCGGCTTTTCCAATGAAACAAAATGGGCAGACGTAATCTGAATAAACCTTGATGTGTACTGTCATGATAATCTCCTTTCGTATAAGTTG                                                  >  1:617999/1‑132
                                                                                          GAGGCTGTCTGTGTCTGTACCGGGAAATTTCTCGCGGGGGAGTCCGGCTTTTCCAATGAAACAAAATGGGCAGACGTAATCTGAATAAACCTTGATGTGTACTGTCATGATAATCTCCTTTCGTATAAGTTG                                                  <  2:617999/132‑1
                                                                                                                                  aaaCCGGCTGCTCCACTGAAACACAATGGGCAGACCTAATCTGAATAAACCTTGATGTGTACTGTCATGATAATCACCTGTCGTATAAGTTGATTTTCAACTGTAACAATTAAAGTTACAAATGTTTTTTTAAAAAGAAG  <  2:2917781/137‑1
                                                                                                                                                                                                                                                                              
CAAAGCCGAGCGCTGCGAAAACACCTGTCACCATCGTCACCTCCACAAAAGTATGTAGGCAAAAGCCTATATGTAAATGTATGTGAGCGGGAGGCTGTCTGTGTCTGTACCGGGAAATTTCTCGTAGGAGAGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/2526508‑2526375
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCTTTTCCAATGAAACAAAATGGGCAGACGTAATCTGAATAAACCTTGATGTGTACTGTCATGATAATCTCCTTTCGTATAAGTTGATTTTAAACTGTAACAATTAAAGTTACAAATGTTTTTTTAAAAAGAAG  >  NZ_CP034943/3751590‑3751725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.