New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 317395275 (1.130)7 (0.030)
+AAC
3/246 NT 2.5% coding (1215/1254 nt) opuAA glycine/proline betaine ABC transporter ATP‑binding protein OpuAA
?NZ_CP034943 317396 = 275 (1.130)coding (1216/1254 nt) opuAA glycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Rejected: Frequency below/above cutoff threshold.

AATGAAAGGGATTGTCGTAAAAGGCGCGCTAATTGGCGCACTCGCCGGCAATAATGATTACATTAATGTTGAAGGCACT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/317317‑317395
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAACAAACACAAGATCCTTCTGTACAGGAGGTGAAGTAAGATGGATAGACTGCCTAGAATACCTTTAGCAGATATGATTGACCGTTTTGTTGACTGGATTACGATGACGTTTGGCG  >  NZ_CP034943/317396‑317511
                                                                               |||                                                                                                                    
AATGAAAGGAATTGTCGTAAGAGGCGCGCTGATTGGCGCACTTGCCGGCAATAACGAGTATATCAATGCTGAAGGCACTAACGAACAAACACAAGATCCTTCTGCACACGAGGTGAAATAAGATGGATAGACTGCCTAGA                                                            <  2:540104/140‑1
  TGAAAGGAATTGTCGTAAGAGGCGCGCTGATTGGCGCACTTGCCGGCAATAACGAGTATATCAATGCTGAAGGCACTAACGAACAAACACAAGATCCTTCTGCACAGGAGGTGAAATAAGATGGATAGACTGCCTAGAAT                                                          <  2:955151/140‑1
                                                          tatatcAATGCTGAAGGCACTAACGAACAAACACAAGATCCTTCTGCACAGGAGGTGAAATAAGATGGATAGACTGCCTAGAATACCTTTAGCAGATATTATTGACCGTTTTGTTGACTGGATTACGATGACGTTTGGCG  >  2:1138524/7‑140
                                                          tatatcAATGCTGAAGGCACTAACGAACAAACACAAGATCCTTCTGCACAGGAGGTGAAATAAGATGGATAGACTGCCTAGAATACCTTTAGCAGATATTATTGACCGTTTTGTTGACTGGATTACGATGACGTTTGGCG  >  2:2528837/7‑140
                                                          tatatcAATGCTGAAGGCACTAACGAACAAACACAAGATCCTTCTGCACAGGAGGTGAAATAAGATGGATAGACTGCCTAGAATACCTTTAGCAGATATTATTGACCGTTTTGTTGACTGGATTACGATGACGTTTGGCG  >  2:602344/7‑140
                                                                  tgcTGAAGGCACTAACGAACAAACACAAGATCCTTCTGCACAGGAGGTGAAATAAGATGGATAGACTGCCTAGAATACCTTTAGCAGATATTATTGA                                     <  1:1979860/94‑1
                                                                  tgcTGAAGGCACTAACGAACAAACACAAGATCCTTCTGCACAGGAGGTGAAATAAGATGGATAGACTGCCTAGAATACCTTTAGCAGATATTATTGA                                     >  2:1979860/4‑97
                                                                               |||                                                                                                                    
AATGAAAGGGATTGTCGTAAAAGGCGCGCTAATTGGCGCACTCGCCGGCAATAATGATTACATTAATGTTGAAGGCACT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/317317‑317395
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAACAAACACAAGATCCTTCTGTACAGGAGGTGAAGTAAGATGGATAGACTGCCTAGAATACCTTTAGCAGATATGATTGACCGTTTTGTTGACTGGATTACGATGACGTTTGGCG  >  NZ_CP034943/317396‑317511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.