New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 2071641 =101 (0.620)31 (0.190) 5/264 8.1 23.9% coding (2652/7377 nt) EO946_RS10385 NTTRR‑F1 domain
?NZ_CP034943 = 2072009 NA (NA)coding (2284/7377 nt) EO946_RS10385 NTTRR‑F1 domain
Rejected: Coverage evenness skew score above cutoff.

AACGGGAGAAACAGGATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/2071782‑2071641
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggaGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  NZ_CP034943/2072009‑2071871
                                                                                                                                                                                                                                                                                         
AACGGGAGAAACAGGATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAG                                                                                                                                               >  2:1117438/1‑140
AACGGGAGAAACAGGATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAG                                                                                                                                               <  1:936520/140‑1
 ACGGGAGAAACAGGATCAACCGGAGCAACTGGAGTAACTGGATCAACGGGAGACACAGGATGAACTGGTGCATCTGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGG                                                                                                                                              <  2:469595/140‑1
 ACGGGAGAAACAGGATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGG                                                                                                                                              <  2:2144809/140‑1
    GGAGAAACAGGATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGC                                                                                                                                           >  2:1894932/1‑140
    GGAGAAACAGGATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGC                                                                                                                                           >  1:1514490/1‑140
    GGAGAAACAGGATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGC                                                                                                                                           >  1:237884/1‑140
      AGAAACAGGATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCA                                                                                                                                         <  1:175030/140‑1
       gacACAGGATCAACCGGCGCAACGGGAGTACCGGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCAC                                                                                                                                        <  1:1332492/137‑1
       GAAACAGGATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGAGCACCTGGAGCCAC                                                                                                                                        >  2:1546812/1‑140
              GATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTA                                                                                                                                 >  2:989929/1‑140
              GATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTA                                                                                                                                 >  1:956700/1‑140
                   ACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGACACAGGATCAACAGGTCCATCGGGATCAACAGGATCACCCGGAGCCAGGGGAGCGACGGGAGCAACAGGATCCACAGGATCACCAGGTGCCCGTGGAGTACCTGG                                                                                                                            >  1:1977610/1‑140
                   ACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACCGGATCAACAGGATCAACAGGAGCCACGGGAGTAACTGG                                                                                                                            >  1:463002/1‑140
                   ACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGG                                                                                                                            >  2:147707/1‑140
                   ACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGG                                                                                                                            >  2:144672/1‑140
                   ACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGG                                                                                                                            >  1:2396462/1‑140
                     CGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGGTG                                                                                                                          <  1:2372069/140‑1
                        AGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCACCAGGAGCCACTGGAGTAACTGGTGCAc                                                                                                                       >  1:1041568/1‑139
                        AGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTCACTGGTGCAA                                                                                                                       >  2:246725/1‑140
                        AGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGGTGCca                                                                                                                       >  2:188873/1‑138
                        AGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGGTGCAc                                                                                                                       >  2:2366440/1‑139
                        AGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGGTGCAA                                                                                                                       >  1:487067/1‑140
                        AGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGGTGCAA                                                                                                                       >  1:1257086/1‑140
                        AGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGCAGCCACGGGAGTACCTGGTGCAc                                                                                                                       >  2:1933949/1‑139
                                GAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGGTGCAACCGGGGAA                                                                                                               >  1:2369080/1‑140
                                  GTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGGTGCAACCGGGGAAAC                                                                                                             <  1:1412345/140‑1
                                     ACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGG                                                                                                                                              <  2:646286/104‑1
                                     ACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGG                                                                                                                                              >  1:646286/1‑104
                                          AGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCA                                                                                                                                         >  2:2036802/1‑104
                                          AGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCA                                                                                                                                         <  1:2036802/104‑1
                                                        acggtTCAACAGGTCCATCGGGATCAACTGGATCAACCGGAGCCACTGGAGTCACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGCGCAACGGGACCAACAGGT                                                                                       <  2:1699014/135‑1
                                                                     TCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGAGCCACGGGAGTAACTGGTGCAACCGGGGAAACTGGAGCAACAGGACCAACAGGTCCAACGGGAGCca                                                                          >  1:1699014/1‑138
                                                                                                      TGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGG                                                                                                                                              >  1:1696315/1‑39
                                                                                                      TGGAGCGACGGGAGAAACAGGATAAACAGGATCAACAGG                                                                                                                                              <  2:1696315/39‑1
                                                                                                                  AGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAA                                                                                                                                <  1:1590789/41‑1
                                                                                                                  AGAAACAGGATCAACAGGATCAACAGGAGCCACTGGAGTAA                                                                                                                                >  2:1590789/1‑41
                                                                                                                            tctACAGGATCACCGGGAGCCTATGGAGTAACTGGTGGATCCGGGGAAACTGGAGGTACTGGAACAACCGCTCCAACGGGAGCAACTGGAGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAAC                   <  2:791921/137‑1
                                                                                                                                       AACAGGAGCAACGGGATCAACTGGTGCAACCGGGGAAACAGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAG        >  2:523946/1‑140
                                                                                                                                            GAGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCC   <  1:1844475/140‑1
                                                                                                                                             AGCCACTGGAGTCACTGGTGGAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  2:2020153/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGTCGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  2:900437/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  1:49400/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  1:1817107/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  1:2297785/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  >  1:2399628/1‑140
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  1:300047/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  1:1099222/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  2:291452/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  >  1:378550/1‑140
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  2:2409300/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  2:1549340/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  2:224385/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  2:2207576/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  1:778127/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  1:1576200/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  2:1543571/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  1:1481304/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  2:1884564/140‑1
                                                                                                                                             AGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACGGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  >  1:63851/1‑140
                                                                                                                                                                                                                                                                                         
AACGGGAGAAACAGGATCAACCGGAGCAACTGGAGTAACTGGAGCAACGGGAGAAACAGGATCAACAGGTCCATCGGGATCAACAGGATCAACCGGAGCCACTGGAGCGACGGGAGAAACAGGATCAACAGGATCAACAGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/2071782‑2071641
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggaGCCACTGGAGTAACTGGTGCAACCGGGGAAACTGGAGCTACAGGACCAACAGGTCCAACGGGAGCAACTGGTGCGACGGGAGCAACGGGAGCAACGGGAGCAACCGGATCAACCGGAGCAACGGGATCAACAGGAGCCA  <  NZ_CP034943/2072009‑2071871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.