mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | SRR10918232_R1.good.fq | 6,546,033 | 985,784,798 | 100.0% | 150.6 bases | 151 bases | 98.2% |
| errors | SRR10918232_R2.good.fq | 6,546,033 | 985,784,798 | 100.0% | 150.6 bases | 151 bases | 95.8% |
| total | 13,092,066 | 1,971,569,596 | 100.0% | 150.6 bases | 151 bases | 97.0% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | CP009974 | 5,798,534 | 332.2 | 2.8 | 100.0% | Pseudomonas putida S12, complete genome. |
| total | 5,798,534 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 100009 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 6 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 1622 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.089 |
| reference sequence | pr(no read start) |
|---|---|
| CP009974 | 0.37805 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 14:53:24 25 Jan 2023 | 14:56:47 25 Jan 2023 | 3 minutes 23 seconds |
| Read alignment to reference genome | 14:56:48 25 Jan 2023 | 15:24:14 25 Jan 2023 | 27 minutes 26 seconds |
| Preprocessing alignments for candidate junction identification | 15:24:14 25 Jan 2023 | 15:27:14 25 Jan 2023 | 3 minutes 0 seconds |
| Preliminary analysis of coverage distribution | 15:27:14 25 Jan 2023 | 15:34:41 25 Jan 2023 | 7 minutes 27 seconds |
| Identifying junction candidates | 15:34:41 25 Jan 2023 | 15:41:19 25 Jan 2023 | 6 minutes 38 seconds |
| Re-alignment to junction candidates | 15:41:19 25 Jan 2023 | 15:52:43 25 Jan 2023 | 11 minutes 24 seconds |
| Resolving best read alignments | 15:52:43 25 Jan 2023 | 15:57:47 25 Jan 2023 | 5 minutes 4 seconds |
| Creating BAM files | 15:57:47 25 Jan 2023 | 16:04:36 25 Jan 2023 | 6 minutes 49 seconds |
| Tabulating error counts | 16:04:36 25 Jan 2023 | 16:07:19 25 Jan 2023 | 2 minutes 43 seconds |
| Re-calibrating base error rates | 16:07:19 25 Jan 2023 | 16:07:20 25 Jan 2023 | 1 second |
| Examining read alignment evidence | 16:07:20 25 Jan 2023 | 16:41:49 25 Jan 2023 | 34 minutes 29 seconds |
| Polymorphism statistics | 16:41:49 25 Jan 2023 | 16:41:51 25 Jan 2023 | 2 seconds |
| Output | 16:41:51 25 Jan 2023 | 16:43:27 25 Jan 2023 | 1 minute 36 seconds |
| Total | 1 hour 50 minutes 2 seconds | ||
