breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | SRR12717895_R1.good.fq | 4,041,000 | 589,897,466 | 100.0% | 146.0 bases | 149 bases | 98.2% |
errors | SRR12717895_R2.good.fq | 4,041,000 | 600,224,484 | 100.0% | 148.5 bases | 149 bases | 97.8% |
total | 8,082,000 | 1,190,121,950 | 100.0% | 147.3 bases | 149 bases | 98.0% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | U00096 | 4,641,652 | 248.1 | 4.1 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 71257 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 607 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.039 |
reference sequence | pr(no read start) |
---|---|
U00096 | 0.46507 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
Junction allow suboptimal matches | FALSE |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
option | value |
---|---|
Mode | Consensus/Mixed Base |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 10 |
Polymorphism frequency cutoff | 0.2 |
Polymorphism minimum variant coverage each strand | OFF |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | OFF |
Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
program | version |
---|---|
bowtie2 | 2.2.6 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 13:57:05 21 Feb 2023 | 13:59:03 21 Feb 2023 | 1 minute 58 seconds |
Read alignment to reference genome | 13:59:03 21 Feb 2023 | 14:13:09 21 Feb 2023 | 14 minutes 6 seconds |
Preprocessing alignments for candidate junction identification | 14:13:09 21 Feb 2023 | 14:14:51 21 Feb 2023 | 1 minute 42 seconds |
Preliminary analysis of coverage distribution | 14:14:51 21 Feb 2023 | 14:19:01 21 Feb 2023 | 4 minutes 10 seconds |
Identifying junction candidates | 14:19:01 21 Feb 2023 | 14:19:06 21 Feb 2023 | 5 seconds |
Re-alignment to junction candidates | 14:19:06 21 Feb 2023 | 14:23:11 21 Feb 2023 | 4 minutes 5 seconds |
Resolving best read alignments | 14:23:11 21 Feb 2023 | 14:25:42 21 Feb 2023 | 2 minutes 31 seconds |
Creating BAM files | 14:25:42 21 Feb 2023 | 14:29:22 21 Feb 2023 | 3 minutes 40 seconds |
Tabulating error counts | 14:29:22 21 Feb 2023 | 14:31:07 21 Feb 2023 | 1 minute 45 seconds |
Re-calibrating base error rates | 14:31:07 21 Feb 2023 | 14:31:08 21 Feb 2023 | 1 second |
Examining read alignment evidence | 14:31:08 21 Feb 2023 | 14:51:44 21 Feb 2023 | 20 minutes 36 seconds |
Polymorphism statistics | 14:51:44 21 Feb 2023 | 14:51:44 21 Feb 2023 | 0 seconds |
Output | 14:51:44 21 Feb 2023 | 14:52:07 21 Feb 2023 | 23 seconds |
Total | 55 minutes 2 seconds |