BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	U00096	3,153,487	Δ15 bp	intergenic (+64/‑62)	metC → / → yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	U00096	3153487	3153501	15	251 [0]	[0] 252	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	= 3153486	0 (0.000)	240 (1.120)	157/280	0.0	100%	intergenic (+63/‑77)	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB
*	?	U00096	3153502 =	0 (0.000)	240 (1.120)	157/280	0.0	100%	intergenic (+79/‑61)	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

Only 100 of 252 total aligned reads displayed.

GCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTACT  >  U00096/3153354‑3153650

gaccttgattcgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccc                                                                                                                                                      <  2:2399910‑M2/1‑1 (MQ=255)

gaccttgattcgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccc                                                                                                                                                      <  2:2399907‑M2/1‑1 (MQ=255)

   cttgattcgcctgcattttggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaaatttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGa                                                                                                                                                   >  2:2400031‑M2/146‑149 (MQ=255)

       attcgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattatataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGAt                                                                                                                                               >  1:2400084‑M2/142‑149 (MQ=255)

        ttcgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGAtt                                                                                                                                              >  1:2400098‑M2/141‑149 (MQ=255)

        ttcgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGAtt                                                                                                                                              >  1:2400001‑M2/141‑149 (MQ=255)

        ttcgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggatttgcgcgaatactataacattgccacttttggacaattttgaagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGAtt                                                                                                                                              >  1:2400073‑M2/141‑149 (MQ=255)

          cgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCa                                                                                                                                            <  1:2399915‑M2/11‑1 (MQ=255)

          cgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCa                                                                                                                                            <  2:2399916‑M2/11‑1 (MQ=255)

            cctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataaccttgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAgg                                                                                                                                          >  2:2400070‑M2/137‑149 (MQ=255)

              tgcatattggtctggaagatgtcgacgttctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAg                                                                                                                                        >  1:2400062‑M2/135‑149 (MQ=255)

              tgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAg                                                                                                                                        <  2:2399920‑M2/15‑1 (MQ=255)

                 atattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTAc                                                                                                                                     >  2:2400024‑M2/132‑149 (MQ=255)

                 atattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTAc                                                                                                                                     <  2:2399923‑M2/18‑1 (MQ=255)

                  tattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACa                                                                                                                                    <  1:2399925‑M2/19‑1 (MQ=255)

                   attggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACaa                                                                                                                                   >  1:2400095‑M2/130‑149 (MQ=255)

                    ttggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAAt                                                                                                                                  >  1:2400035‑M2/129‑149 (MQ=255)

                     tggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATa                                                                                                                                 <  2:2399927‑M2/22‑1 (MQ=255)

                      ggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAg                                                                                                                                >  1:2400047‑M2/127‑149 (MQ=255)

                       gtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAgg                                                                                                                               <  2:2399929‑M2/24‑1 (MQ=255)

                        tctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGc                                                                                                                              >  1:2400139‑M2/125‑149 (MQ=255)

                           ggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGc                                                                                                                              >  2:2400017‑M2/122‑146 (MQ=255)

                             aagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATa                                                                                                                         <  2:2399935‑M2/30‑1 (MQ=255)

                              agatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATaa                                                                                                                        >  1:2400077‑M2/119‑149 (MQ=255)

                                atgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATaaa                                                                                                                       <  1:2399936‑M2/32‑1 (MQ=255)

                                atgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAg                                                                                                                      <  1:2399938‑M2/33‑1 (MQ=255)

                                    cgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccTGGACGATTCAGGAGTACAATAGGCAGATAAAGGCt                                                                                                                   <  1:2399943‑M2/36‑1 (MQ=255)

                                    cgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCtt                                                                                                                  <  2:2399942‑M2/37‑1 (MQ=255)

                                     gacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTa                                                                                                                 >  1:2400109‑M2/112‑149 (MQ=255)

                                      acgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTaa                                                                                                                <  2:2399947‑M2/39‑1 (MQ=255)

                                      acgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTaa                                                                                                                <  2:2399949‑M2/39‑1 (MQ=255)

                                      acgatcggattgccgatctggacgccggttatgcgagaattgtataacattgtcacttttggagaattttgcagacattttattgtgaaaagtcttaaaatgttgcgtcaCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTaa                                                                                                                >  2:2400059‑M2/111‑149 (MQ=255)

                                          tctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCa                                                                                                                                            >  1:2399950‑M2/107‑117 (MQ=255)

                                          tctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGc                                                                                                            >  1:2400144‑M2/107‑149 (MQ=255)

                                           ctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCt                                                                                                           <  1:2399954‑M2/44‑1 (MQ=255)

                                             gattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACg                                                                                                             <  2:2399956‑M2/42‑1 (MQ=255)

                                              attgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGtt                                                                                                        >  2:2400009‑M2/103‑149 (MQ=255)

                                              attgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGtt                                                                                                        >  1:2400117‑M2/103‑149 (MQ=255)

                                                tgccgatctggacgccggtgttgcgcgaattgtataaccttgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattggtgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTcc                                                                                                      <  2:2399959‑M2/49‑1 (MQ=255)

                                                 gccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCca                                                                                                     >  2:2400093‑M2/100‑149 (MQ=255)

                                                        tggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGaaa                                                                                              <  1:2399964‑M2/57‑1 (MQ=255)

                                                        tggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGaaa                                                                                              <  2:2399962‑M2/57‑1 (MQ=255)

                                                         ggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAgg                                                                                                 <  2:2399965‑M2/54‑1 (MQ=255)

                                                         ggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAg                                                                                             >  2:2400126‑M2/92‑149 (MQ=255)

                                                          gacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGt                                                                                            <  1:2399969‑M2/59‑1 (MQ=255)

                                                             gccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCa                                                                                         <  2:2399971‑M2/62‑1 (MQ=255)

                                                              ccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCAt                                                                                        <  2:2399974‑M2/63‑1 (MQ=255)

                                                              ccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCAt                                                                                        <  1:2399973‑M2/63‑1 (MQ=255)

                                                                  ttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCt                                                                                    >  1:2400105‑M2/83‑149 (MQ=255)

                                                                    ttgcgcgaattgtataacattggcacttttggagaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGCAGTACAATAGGCAGATAATGGCTTTAACGCTGTTCGACAGGAAAGTCCATGGCTGt                                                                                  <  2:2399977‑M2/69‑1 (MQ=255)

                                                                        gcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTa                                                                                <  1:2399978‑M2/71‑1 (MQ=255)

                                                                        gcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTAtt                                                                              >  2:2400065‑M2/77‑149 (MQ=255)

                                                                         cgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTc                                                                             >  2:2400054‑M2/76‑149 (MQ=255)

                                                                          gaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCa                                                                            >  1:2400049‑M2/75‑149 (MQ=255)

                                                                           aattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCaa                                                                           <  2:2399982‑M2/76‑1 (MQ=255)

                                                                           aattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCaa                                                                           <  1:2399980‑M2/76‑1 (MQ=255)

                                                                            attgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAg                                                                          <  1:2399984‑M2/77‑1 (MQ=255)

                                                                                  taacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatca                                                                    <  2:2399988‑M2/83‑1 (MQ=255)

                                                                                  taacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatca                                                                    <  1:2399989‑M2/83‑1 (MQ=255)

                                                                                   aacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcat                                                                   >  1:2400135‑M2/66‑149 (MQ=255)

                                                                                    acattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcatc                                                                  <  2:2399991‑M2/85‑1 (MQ=255)

                                                                                    acattgcaacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTGAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcatc                                                                  >  2:2400044‑M2/65‑149 (MQ=255)

                                                                                      attgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGc                                                                >  1:2400069‑M2/63‑149 (MQ=255)

                                                                                       ttgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCt                                                               <  1:2399994‑M2/88‑1 (MQ=255)

                                                                                        tgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTg                                                              <  2:2399996‑M2/89‑1 (MQ=255)

                                                                                         gccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgc                                                             >  2:2400160‑M2/60‑149 (MQ=255)

                                                                                           cacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTa                                                                                                                 <  1:2399997‑M2/38‑1 (MQ=255)

                                                                                           cacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgcgc                                                           >  2:2400137‑M2/58‑149 (MQ=255)

                                                                                                 tggacaattttgcacacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGc                                                     >  2:2400108‑M2/52‑149 (MQ=255)

                                                                                                     caattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAaca                                                 <  2:2400001‑M2/102‑1 (MQ=255)

                                                                                                     caattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAaca                                                 <  1:2400003‑M2/102‑1 (MQ=255)

                                                                                                         tttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGAc                                             <  2:2400006‑M2/106‑1 (MQ=255)

                                                                                                           tgcagagattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACtt                                           >  1:2400007‑M2/42‑149 (MQ=255)

                                                                                                              agacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc                                        <  1:2400011‑M2/111‑1 (MQ=255)

                                                                                                               gacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGcc                                       <  2:2400012‑M2/112‑1 (MQ=255)

                                                                                                                acattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTATGCcg                                      >  1:2400170‑M2/37‑149 (MQ=255)

                                                                                                                   ttttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGc                                                     >  1:2400164‑M2/34‑131 (MQ=255)

                                                                                                                   ttttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc                                        >  1:2400207‑M2/34‑144 (MQ=255)

                                                                                                                   ttttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                                   >  2:2400113‑M2/34‑149 (MQ=255)

                                                                                                                    tttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                                   >  1:2400089‑M2/33‑148 (MQ=255)

                                                                                                                    tttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt                                  <  1:2400017‑M2/117‑1 (MQ=255)

                                                                                                                      tattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTgg                                <  2:2400019‑M2/119‑1 (MQ=255)

                                                                                                                        ttgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCg                              <  1:2400020‑M2/121‑1 (MQ=255)

                                                                                                                            gaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCCTGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACCCGACTTTGCCGCGCTGGCGGATc                          >  2:2400133‑M2/25‑149 (MQ=255)

                                                                                                                            gaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATc                          >  1:2400198‑M2/25‑149 (MQ=255)

                                                                                                                                 gtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCat                     >  2:2400151‑M2/20‑149 (MQ=255)

                                                                                                                                  tcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCata                    >  1:2400190‑M2/19‑149 (MQ=255)

                                                                                                                                   cttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCatat                   <  1:2400037‑M2/132‑1 (MQ=255)

                                                                                                                                    ttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATAtt                  >  2:2400119‑M2/17‑149 (MQ=255)

                                                                                                                                     taaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATAtt                  >  1:2400028‑M2/16‑148 (MQ=255)

                                                                                                                                     taaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTg                 <  1:2400031‑M2/134‑1 (MQ=255)

                                                                                                                                       aattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGtt               >  2:2400205‑M2/14‑149 (MQ=255)

                                                                                                                                        attgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAACGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTa              >  2:2400026‑M2/13‑149 (MQ=255)

                                                                                                                                        attgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTa              <  2:2400035‑M2/137‑1 (MQ=255)

                                                                                                                                             tgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgtt         <  1:2400024‑M2/142‑1 (MQ=255)

                                                                                                                                                 tccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATGGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGttt     >  1:2400124‑M2/4‑149 (MQ=255)

                                                                                                                                                 tccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGttt     >  2:2400156‑M2/4‑149 (MQ=255)

                                                                                                                                                 tccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGttt     >  2:2400172‑M2/4‑149 (MQ=255)

                                                                                                                                                   cCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCGGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTAc   <  2:2400040‑M2/148‑1 (MQ=255)

                                                                                                                                                    cGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTACt  >  2:2400085/1‑149 (MQ=255)

GCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTACT  >  U00096/3153354‑3153650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`