Predicted mutation
evidence seq id position mutation annotation gene description
MC JC AP012306 87,679 (GCG)4→3 coding (1442‑1444/1767 nt) ftsI → transpeptidase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ AP012306 87669 87671 3 255 [0] [0] 259 ftsI transpeptidase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 87672 =0 (0.000)233 (0.810) 153/278 0.6 100% coding (1435/1767 nt) ftsI transpeptidase
?AP012306 = 87668 0 (0.000)coding (1431/1767 nt) ftsI transpeptidase

Only 100 of 259 total aligned reads displayed.

CGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTG  >  AP012306/87523‑87821
                                                                                                                                                     |                                                                                                                                                     
tagcgcgagtctacgcaactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccag                                                                                                                                                       >  2:1624753‑M1/150‑150 (MQ=255)
tagcgcgagtctacgcaactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccag                                                                                                                                                       >  1:2131514‑M1/150‑150 (MQ=255)
tagcgcgagtctacgcaactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccag                                                                                                                                                       <  2:3986417‑M1/1‑1 (MQ=255)
 agcgcgagtctacgcaactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccagg                                                                                                                                                      >  1:3780562‑M1/149‑150 (MQ=255)
 agcgcgagtctacgcaactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccagg                                                                                                                                                      >  2:856981‑M1/149‑150 (MQ=255)
       agtctacgcaactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaggcggcgg                                                                                                                                                <  1:2901266‑M1/8‑1 (MQ=255)
        gtctacgcaactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaggcggcggc                                                                                                                                               >  2:3745943‑M1/142‑150 (MQ=255)
          ctacgcaactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGt                                                                                                                                             <  1:2303803‑M1/11‑1 (MQ=255)
           tacgcaactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTg                                                                                                                                            >  1:1318666‑M1/139‑150 (MQ=255)
           tacgcaactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTg                                                                                                                                            <  1:3594533‑M1/12‑1 (MQ=255)
              gcaactatcggcggctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAg                                                                                                                                         >  2:526285‑M1/136‑150 (MQ=255)
               caactatcggcagctacggcatttatcgcccactgtcgattaccaaagttggccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAgg                                                                                                                                        >  1:707069‑M1/135‑150 (MQ=255)
               caactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAgg                                                                                                                                        <  1:1233070‑M1/16‑1 (MQ=255)
                aactatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAggc                                                                                                                                       <  1:1645619‑M1/17‑1 (MQ=255)
                 actatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAggcg                                                                                                                                      <  2:2620274‑M1/18‑1 (MQ=255)
                 actatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAggcg                                                                                                                                      <  2:691842‑M1/18‑1 (MQ=255)
                   tatcggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAggcggc                                                                                                                                    >  2:278795‑M1/131‑150 (MQ=255)
                       ggcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGAtt                                                                                                                                >  1:2751508‑M1/127‑150 (MQ=255)
                        gcagctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTa                                                                                                                               >  2:1907227‑M1/126‑150 (MQ=255)
                          agctacggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTaaa                                                                                                                             <  2:661728‑M1/27‑1 (MQ=255)
                               cggcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCta                                                                                                                        >  1:1391243‑M1/119‑150 (MQ=255)
                                 gcatttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatc                                                                                                                      <  2:2734991‑M1/34‑1 (MQ=255)
                                  catttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcg                                                                                                                     <  1:2738198‑M1/35‑1 (MQ=255)
                                    tttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgta                                                                                                                   <  2:3405267‑M1/37‑1 (MQ=255)
                                     ttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtat                                                                                                                  >  1:4001595‑M1/113‑150 (MQ=255)
                                     ttatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtat                                                                                                                  >  2:1935081‑M1/113‑150 (MQ=255)
                                      tatcgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtatc                                                                                                                 <  2:2380435‑M1/39‑1 (MQ=255)
                                         cgcccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGcc                                                                                                              >  2:2826220‑M1/109‑150 (MQ=255)
                                           cccactgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCAt                                                                                                            >  2:3753703‑M1/107‑150 (MQ=255)
                                               ctgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaaa                                                                                                        <  2:1743855‑M1/48‑1 (MQ=255)
                                                tgtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaaaa                                                                                                       >  1:3669361‑M1/102‑150 (MQ=255)
                                                 gtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAAc                                                                                                      <  1:3451327‑M1/50‑1 (MQ=255)
                                                 gtcgattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAAc                                                                                                      <  1:3505153‑M1/50‑1 (MQ=255)
                                                    gattaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCgg                                                                                                   <  2:245733‑M1/53‑1 (MQ=255)
                                                     attaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGt                                                                                                  >  1:2687838‑M1/97‑150 (MQ=255)
                                                      ttaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTa                                                                                                 >  2:3637681‑M1/96‑150 (MQ=255)
                                                      ttaccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTa                                                                                                 <  1:517639‑M1/55‑1 (MQ=255)
                                                       taccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAc                                                                                                >  1:817682‑M1/95‑150 (MQ=255)
                                                       taccaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAc                                                                                                >  1:1040432‑M1/95‑150 (MQ=255)
                                                        accaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAcc                                                                                               >  2:3959964‑M1/94‑150 (MQ=255)
                                                        accaaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAcc                                                                                               >  2:3233085‑M1/94‑150 (MQ=255)
                                                          caaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACcgc                                                                                             <  1:1138851‑M1/59‑1 (MQ=255)
                                                          caaagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACcgc                                                                                             <  2:2122459‑M1/59‑1 (MQ=255)
                                                            aagttgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGa                                                                                           <  1:157492‑M1/61‑1 (MQ=255)
                                                                tgaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGaaaaa                                                                                       <  1:934436‑M1/65‑1 (MQ=255)
                                                                 gaccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAg                                                                                      >  1:2896033‑M1/85‑150 (MQ=255)
                                                                   ccccccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGt                                                                                    >  2:20514‑M1/83‑150 (MQ=255)
                                                                      cccggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCgg                                                                                 >  1:910629‑M1/80‑150 (MQ=255)
                                                                        cggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGc                                                                               <  1:1935734‑M1/73‑1 (MQ=255)
                                                                         ggttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGcc                                                                              <  1:2419183‑M1/74‑1 (MQ=255)
                                                                          gttcccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCg                                                                             >  2:3358318‑M1/76‑150 (MQ=255)
                                                                             cccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGAc                                                                          <  1:2068323‑M1/78‑1 (MQ=255)
                                                                              ccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACg                                                                         <  2:1509002‑M1/79‑1 (MQ=255)
                                                                              ccggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACg                                                                         >  2:1836208‑M1/72‑150 (MQ=255)
                                                                               cggtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACgg                                                                        >  1:1506241‑M1/71‑150 (MQ=255)
                                                                                 gtgaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTc                                                                      >  2:2571766‑M1/69‑150 (MQ=255)
                                                                                   gaacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGc                                                                    <  1:2773266‑M1/84‑1 (MQ=255)
                                                                                    aacgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCt                                                                   >  2:3551920‑M1/66‑150 (MQ=255)
                                                                                     acgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTa                                                                  >  2:5626‑M1/65‑150 (MQ=255)
                                                                                     acgtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTa                                                                  <  2:1825902‑M1/86‑1 (MQ=255)
                                                                                       gtgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACa                                                                >  2:241643‑M1/63‑150 (MQ=255)
                                                                                        tgtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACAt                                                               >  2:3879339‑M1/62‑150 (MQ=255)
                                                                                         gtcttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATc                                                              >  1:3952536‑M1/61‑150 (MQ=255)
                                                                                           cttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaa                                                            >  1:3080144‑M1/59‑150 (MQ=255)
                                                                                            ttcccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaat                                                           >  1:1012465‑M1/58‑150 (MQ=255)
                                                                                              cccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaataa                                                         >  1:2084336‑M1/56‑150 (MQ=255)
                                                                                               ccggaatccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaataaa                                                        >  1:3352561‑M1/55‑150 (MQ=255)
                                                                                                    atccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAatatat                                                   <  1:3169782‑M1/101‑1 (MQ=255)
                                                                                                      ccattgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTg                                                 >  1:2515076‑M1/48‑150 (MQ=255)
                                                                                                        attgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCt                                               >  2:27208‑M1/46‑150 (MQ=255)
                                                                                                         ttgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCtt                                              >  2:4134355‑M1/45‑150 (MQ=255)
                                                                                                         ttgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCtt                                              >  2:1173042‑M1/45‑150 (MQ=255)
                                                                                                         ttgtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCtt                                              >  2:2295740‑M1/45‑150 (MQ=255)
                                                                                                           gtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTtat                                            <  1:1745624‑M1/108‑1 (MQ=255)
                                                                                                           gtccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTtat                                            <  1:2820282‑M1/108‑1 (MQ=255)
                                                                                                            tccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTtata                                           >  2:1902243‑M1/42‑150 (MQ=255)
                                                                                                            tccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTtata                                           >  1:2551324‑M1/42‑150 (MQ=255)
                                                                                                             ccgcactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATAc                                          >  2:797406‑M1/41‑150 (MQ=255)
                                                                                                                 actgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCa                                      <  1:2254714‑M1/114‑1 (MQ=255)
                                                                                                                    gtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGc                                   >  1:190206‑M1/34‑150 (MQ=255)
                                                                                                                    gtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGc                                   >  2:3698527‑M1/34‑150 (MQ=255)
                                                                                                                       gtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGtt                                <  2:980324‑M1/120‑1 (MQ=255)
                                                                                                                        tgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTTAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTg                               >  2:1489721‑M1/30‑150 (MQ=255)
                                                                                                                          catatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTgcg                             <  2:1675350‑M1/123‑1 (MQ=255)
                                                                                                                             atgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCt                          <  2:1008287‑M1/126‑1 (MQ=255)
                                                                                                                              tgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTg                         >  1:3232666‑M1/24‑150 (MQ=255)
                                                                                                                                atggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCg                       >  2:1324265‑M1/22‑150 (MQ=255)
                                                                                                                                atggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTAAATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCg                       >  2:1374155‑M1/22‑150 (MQ=255)
                                                                                                                                atggaaagcgtggcgctaccaGGCGGCGGCGTGAAGCCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCg                       <  1:4153433‑M1/129‑1 (MQ=255)
                                                                                                                                 tggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGa                      <  2:2897436‑M1/130‑1 (MQ=255)
                                                                                                                                  ggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAg                     <  1:646815‑M1/131‑1 (MQ=255)
                                                                                                                                         gtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCcg              <  1:4071871‑M1/138‑1 (MQ=255)
                                                                                                                                          tggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCcgc             >  2:3839535‑M1/12‑150 (MQ=255)
                                                                                                                                          tggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCcgc             >  2:2199875‑M1/12‑150 (MQ=255)
                                                                                                                                           ggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCcgcg            <  1:2439165‑M1/140‑1 (MQ=255)
                                                                                                                                            gcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCgcgc           <  1:635336‑M1/141‑1 (MQ=255)
                                                                                                                                              gctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCtt         <  2:3129149‑M1/143‑1 (MQ=255)
                                                                                                                                              gctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCtt         <  2:1964723‑M1/143‑1 (MQ=255)
                                                                                                                                                 accaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTcgc      >  1:3823099‑M1/5‑150 (MQ=255)
                                                                                                                                                     GGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTg  >  2:672190‑M1/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTG  >  AP012306/87523‑87821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑