New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_012967 = 2278812191 (0.880)3 (0.020) 3/128 20.8 1.6% coding (2201/4515 nt) ECB_RS11350 alpha‑2‑macroglobulin
?NC_012967 = 2278856 205 (1.050)coding (2157/4515 nt) ECB_RS11350 alpha‑2‑macroglobulin
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 224 total aligned reads displayed.

GGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCAC  >  NC_012967/2278785‑2278927
                                                                       |                                                                       
ggCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTc                                                                         >  2:6339569/1‑72 (MQ=255)
ggCGGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACGTTGATCCCGGCGTTc                                                                         <  2:172745/72‑1 (MQ=255)
  cTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTg                                                                       >  2:4410821/1‑72 (MQ=255)
     gTAATGGGTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGaaa                                                                    <  1:6052067/72‑1 (MQ=255)
      tAATGGGTTTTGTCCGTTGTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGaaaa                                                                   <  1:1636587/72‑1 (MQ=255)
       agtGGGTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAAc                                                                  <  1:3374078/70‑1 (MQ=255)
       aaTGGGTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAAc                                                                  <  1:7429712/72‑1 (MQ=255)
          gggTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAANTTTGATCCCGGCGTTCTGAAAACttt                                                               >  2:812528/1‑70 (MQ=255)
          gggTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGt                                                               <  1:4026026/72‑1 (MQ=255)
           ggTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTa                                                              >  1:3465703/1‑72 (MQ=255)
           ggTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTa                                                              >  1:3056249/1‑72 (MQ=255)
              tttGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCAGGCGTTCTGAAAACTGTACTg                                                           <  1:6437083/72‑1 (MQ=255)
               ttGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTGTGATCCCGGCGTTCTGAAAACTGTACTGa                                                          <  2:3705527/72‑1 (MQ=255)
                tGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGAc                                                         <  1:4489212/72‑1 (MQ=255)
                tGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGAc                                                         <  2:2328683/72‑1 (MQ=255)
                 gTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGAcc                                                        <  2:3250355/72‑1 (MQ=255)
                  tCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCa                                                       >  1:6045457/1‑72 (MQ=255)
                   ccGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCAt                                                      >  1:3865041/1‑72 (MQ=255)
                   ccGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCAt                                                      <  2:6840752/72‑1 (MQ=255)
                   ccGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCAt                                                      >  1:6563399/1‑72 (MQ=255)
                   ccGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCAt                                                      >  2:6132326/1‑72 (MQ=255)
                     gTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTa                                                    >  2:5732344/1‑72 (MQ=255)
                     gTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTa                                                    <  1:6665351/72‑1 (MQ=255)
                     gTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTa                                                    <  2:3273295/72‑1 (MQ=255)
                     gTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTa                                                    >  1:2183745/1‑72 (MQ=255)
                     gTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTa                                                    >  2:970949/1‑72 (MQ=255)
                     gTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTa                                                    >  2:2412717/1‑72 (MQ=255)
                       tttCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACg                                                  >  2:4910117/1‑72 (MQ=255)
                        ttCACTCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACgg                                                 <  2:2540215/72‑1 (MQ=255)
                        ntCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACgg                                                 <  2:2812276/71‑1 (MQ=255)
                           aCCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGta                                              >  1:5909052/1‑72 (MQ=255)
                            cccGGATATCAAGCTGAGGAACGGCAACTTTGATCACGGCGTTCTGAAAACTGTACTGACCATTACGGGtat                                             <  1:1110144/72‑1 (MQ=255)
                             ccGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTNCTGACCATTACGGGtata                                            >  1:2506012/1‑72 (MQ=255)
                              cGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATAc                                           >  1:7337503/1‑72 (MQ=255)
                              cGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATAc                                           >  1:2217378/1‑72 (MQ=255)
                              cGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGCATAc                                           >  2:1654715/1‑72 (MQ=255)
                               ggATATCAAGCTGAGGAACGGCAACTTTGNTCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATCCa                                          >  2:625173/1‑72 (MQ=255)
                               ggATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACa                                          <  2:7428988/72‑1 (MQ=255)
                                gATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTCCTGACCATTACGGGTATACAg                                         >  2:424601/1‑72 (MQ=255)
                                 atatCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGc                                        >  2:4530073/1‑72 (MQ=255)
                                 atatCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGc                                        >  1:7408792/1‑72 (MQ=255)
                                 atatCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGc                                        >  1:1499148/1‑72 (MQ=255)
                                 atatCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGc                                        >  2:3361347/1‑72 (MQ=255)
                                 atatCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATNACGGGTATACAGc                                        <  1:2406860/72‑1 (MQ=255)
                                  tatCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACTCTTACGGGTGTACAGCa                                       >  2:949030/1‑72 (MQ=255)
                                   atCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCAc                                      <  1:3158085/72‑1 (MQ=255)
                                   atCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCAc                                      <  2:5281996/72‑1 (MQ=255)
                                    tCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCAcc                                     >  2:2338336/1‑72 (MQ=255)
                                      aaGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGa                                   <  1:6765501/72‑1 (MQ=255)
                                      aaGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGa                                   <  1:2939454/72‑1 (MQ=255)
                                      aaGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGa                                   >  1:4895673/1‑72 (MQ=255)
                                      aaGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGa                                   >  1:6008531/1‑72 (MQ=255)
                                       aGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGaa                                  >  2:4345333/1‑72 (MQ=255)
                                       aGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGaa                                  <  1:2612112/72‑1 (MQ=255)
                                        gCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGaac                                 >  2:1397942/1‑71 (MQ=255)
                                          tGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGaaaaa                               <  2:7313117/72‑1 (MQ=255)
                                            aGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACCGTACTGACCATTACGGGTATACAGCACCGAAAAAGt                             >  2:3211327/1‑72 (MQ=255)
                                             ggAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGTGTATACAGCACCGAAAAAGTg                            >  1:5236670/1‑72 (MQ=255)
                                               aaCGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGAt                          >  2:5871299/1‑72 (MQ=255)
                                               aaCGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGAt                          <  2:2044926/72‑1 (MQ=255)
                                               aaCGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGAt                          >  2:6578148/1‑72 (MQ=255)
                                               aaCGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGAt                          <  1:5369246/72‑1 (MQ=255)
                                               aaCGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACAATTACGGGTATACAGCACCGAAAAAGTGAt                          >  2:7139060/1‑72 (MQ=255)
                                                  ggCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGtt                       >  1:1168678/1‑72 (MQ=255)
                                                  ggCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGtt                       >  1:7429815/1‑72 (MQ=255)
                                                   gCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTa                      <  1:3731510/72‑1 (MQ=255)
                                                   gCAACTTTGATCCCGGCGNTCTGAAAACTGTACTGACCNTTACGGGTATACANNACCGAAAAAGTGATGTTa                      <  2:266033/72‑1 (MQ=255)
                                                    cAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAg                     >  1:3391874/1‑72 (MQ=255)
                                                    cAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAg                     <  2:1415975/72‑1 (MQ=255)
                                                    cAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAg                     <  1:5836554/72‑1 (MQ=255)
                                                     aaCTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAgg                    <  2:2001290/72‑1 (MQ=255)
                                                     aaCTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAgg                    >  2:1147897/1‑72 (MQ=255)
                                                      aCTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGgc                   <  1:4593631/72‑1 (MQ=255)
                                                      aCTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGgc                   >  1:472740/1‑72 (MQ=255)
                                                       cTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcg                  <  1:65385/72‑1 (MQ=255)
                                                       cTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcg                  <  1:1792415/72‑1 (MQ=255)
                                                       cTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcg                  <  2:5157870/72‑1 (MQ=255)
                                                       cTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcg                  <  1:7302795/72‑1 (MQ=255)
                                                        tttGATCCCGGCGTTCTGAAAACTGTACTGATCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcgc                 >  2:1054465/1‑72 (MQ=255)
                                                        tttGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcgc                 <  2:4646507/72‑1 (MQ=255)
                                                        tttGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcgc                 <  1:2112055/72‑1 (MQ=255)
                                                        tttGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcgc                 <  1:4205780/72‑1 (MQ=255)
                                                          tGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAANAAGTGATGTTAGGCGCaa               >  1:2915538/1‑72 (MQ=255)
                                                           gATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCaaa              >  2:871719/1‑72 (MQ=255)
                                                              cccGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGa           >  2:5989273/1‑72 (MQ=255)
                                                              cccGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGa           <  2:2123358/72‑1 (MQ=255)
                                                               ccGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGaa          <  2:3819930/72‑1 (MQ=255)
                                                               ccGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGaa          >  1:1954527/1‑72 (MQ=255)
                                                                cGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAAt         >  1:7271971/1‑72 (MQ=255)
                                                                 ggCGTTCTGAAAACTGTACTGACCATTACTGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGANtt        >  2:608924/1‑72 (MQ=255)
                                                                 ggCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAAtt        >  2:1318802/1‑72 (MQ=255)
                                                                 ggCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAAtt        >  2:4057323/1‑72 (MQ=255)
                                                                  gCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTg       <  2:2638052/72‑1 (MQ=255)
                                                                   cGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGc      >  2:5528197/1‑72 (MQ=255)
                                                                    gTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCTCAAAGGAATTGCt     <  2:2742287/72‑1 (MQ=255)
                                                                    gTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCt     >  1:5092774/1‑72 (MQ=255)
                                                                    gTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCt     <  2:5131304/72‑1 (MQ=255)
                                                                     ttCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTc    <  1:2640171/72‑1 (MQ=255)
                                                                       cTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCAc  >  2:2205419/1‑72 (MQ=255)
                                                                       cTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCAc  <  1:5485133/72‑1 (MQ=255)
                                                                       |                                                                       
GGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCAAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCATTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCAC  >  NC_012967/2278785‑2278927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.