breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 119,500 A→C intergenic (+157/‑4) pdhR → / → aceE pyruvate dehydrogenase complex transcriptional repressor PdhR/pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
MC JC 1,290,924 2651 bp→80 bp coding (4‑2654/2676 nt) adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
MC JC 1,749,959 1388 bp→80 bp coding (5‑1392/1413 nt) pykF → pyruvate kinase PykF
MC JC 1,931,909 1419 bp→81 bp coding (4‑1422/1443 nt) pyk → pyruvate kinase
RA 3,081,838 G→A A66T (GCA→ACA)  galP → galactose/proton symporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 255803 256027 225 52 [50] [49] 52 [phoE] [phoE]
* * ÷ NZ_CP009273 578573 579155 583 53 [48] [49] 52 [BW25113_RS26085]–[appY] [BW25113_RS26085],[appY]
* * ÷ NZ_CP009273 748254 748661 408 52 [50] [50] 52 [gltA] [gltA]
* * ÷ NZ_CP009273 946672 949096 2425 51 [50] [49] 52 pflB–[focA] pflB,[focA]
* * ÷ NZ_CP009273 1524242 1524739 498 52 [50] [47] 52 BW25113_RS26145–[BW25113_RS25600] BW25113_RS26145,[BW25113_RS25600]
* * ÷ NZ_CP009273 1972971 1973160 190 106 [0] [50] 51 flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* * ÷ NZ_CP009273 2462577 2463411 835 51 [50] [49] 51 [yfdH]–[BW25113_RS12270] [yfdH],[BW25113_RS12270]
* * ÷ NZ_CP009273 2463508 2463986 479 53 [49] [47] 59 [BW25113_RS12270]–[BW25113_RS26185] [BW25113_RS12270],[BW25113_RS26185]
* * ÷ NZ_CP009273 3762856 3763589 734 52 [45] [49] 51 [BW25113_RS26240] [BW25113_RS26240]
* * ÷ NZ_CP009273 3797557 3798589 1033 51 [50] [50] 52 [rfaS]–[rfaP] [rfaS],[rfaP]
* * ÷ NZ_CP009273 4127770 4129012 1243 52 [49] [45] 54 gldA–[fsa] gldA,[fsa]
* * ÷ NZ_CP009273 4546125 4546569 445 52 [44] [49] 57 [yjiC] [yjiC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =61 (0.530)77 (0.670) 51/474 1.4 56.2% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 59 (0.520)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =43 (0.380)91 (0.850) 65/442 0.4 70.8% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 35 (0.330)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326143 (0.380)95 (0.890) 73/442 0.2 71.7% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 35 (0.330)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 19729700 (0.000)105 (0.920) 66/474 0.5 100% intergenic (‑516/‑264) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP