breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 9.9% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 128,120 G→T 13.8% pseudogene (76/106 nt) mgtT → protein MgtT
RA NZ_CP022686 235,846 A→C 7.0% N287T (AAC→ACC)  pykF → pyruvate kinase PykF
RA NZ_CP022686 238,324 G→C 18.8% V85V (GTC→GTG sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 298,027 A→C 10.6% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 311,464 A→C 9.2% E119D (GAA→GAC ydjY → lipoprotein YdjY
RA NZ_CP022686 349,106 A→C 10.3% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 355,148 T→G 5.9% G139G (GGT→GGG
D270A (GAC→GCC) 
yeaL →
nimR ←
DUF441 domain‑containing protein
DNA‑binding transcriptional regulator NimR
RA NZ_CP022686 360,227 T→G 17.4% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 377,307 A→C 7.8% K147N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 418,374 T→G 8.2% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,086 A→C 11.8% L399V (TTA→GTA)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 498,662 A→C 9.8% C183G (TGT→GGT)  araH ← arabinose ABC transporter permease AraH
RA NZ_CP022686 556,940 A→T 15.8% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 22.4% V1607G (GTC→GGC) ‡ irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,296 C→G 9.2% V1607V (GTC→GTG) ‡ irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 22.6% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,337 T→G 15.1% V1621G (GTT→GGT)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 684,585 A→C 12.1% intergenic (‑946/‑234) CIW80_RS26355 ← / → CIW80_RS03520 IS4 family transposase/TonB‑dependent receptor
RA NZ_CP022686 693,001 A→C 7.5% N114T (AAC→ACC)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 693,005 A→C 7.7% L115L (CTA→CTC CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 693,021 A→C 9.4% T121P (ACG→CCG)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 694,391 C→A 7.7% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,438 G→T 11.2% M1M (ATG→ATT) † CIW80_RS03560 → PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,448 T→A 11.0% intergenic (‑37/+190) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 10.8% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 16.9% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 864,173 T→G 18.4% intergenic (+39/+2) yehR → / ← yehS YehR family lipoprotein/DUF1456 family protein
RA NZ_CP022686 881,733 A→C 16.2% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,742 A→C 18.7% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,023 G→T 10.9% intergenic (+92/‑165) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 15.6% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 12.4% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,439 T→G 24.6% P942P (CCA→CCC yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,073 A→C 7.5% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 13.0% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,374 A→T 7.6% intergenic (‑223/‑50) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,065,978 A→C 8.0% F37V (TTT→GTT)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,065,997 A→C 8.8% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,959 T→G 8.2% D182A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,209,908 T→G 14.0% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,209,935 A→C 17.6% V387G (GTG→GGG)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,479 T→G 21.8% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,232,494 A→C 6.1% E635D (GAA→GAC acrD → multidrug efflux RND transporter permease AcrD
RA NZ_CP022686 1,237,918 C→A 11.1% C303F (TGC→TTC)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,239,841 A→C 17.0% intergenic (‑138/+30) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,360,709 T→G 11.3% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 8.3% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,756 C→A 8.9% intergenic (‑91/+233) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,762 G→T 7.6% intergenic (‑97/+227) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,366,270 T→G 6.0% intergenic (‑271/+171) CIW80_RS06740 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA NZ_CP022686 1,366,277 T→G 9.0% intergenic (‑278/+164) CIW80_RS06740 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA NZ_CP022686 1,370,568 G→T 15.0% V95V (GTC→GTA rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,441,726 A→C 8.3% L612V (TTA→GTA)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,484,920 C→A 9.2% intergenic (‑255/+10) CIW80_RS07420 ← / ← cysH Hok/Gef family protein/phosphoadenosine phosphosulfate reductase
RA NZ_CP022686 1,504,289 T→G 9.0% R242R (CGT→CGG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,310 G→T 11.9% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,313 C→G 6.8% C250W (TGC→TGG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,324 C→A 12.0% P254H (CCT→CAT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 10.0% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 21.3% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,546,995 G→T 19.1% intergenic (+17/‑17) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,000 G→C 9.6% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,110 G→C 20.6% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,113 G→C 17.5% intergenic (+25/‑9) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,377 G→T 11.6% P458P (CCG→CCT vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,558,395 A→C 14.1% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,582,998 T→G 5.6% T533P (ACG→CCG)  recD ← exodeoxyribonuclease V subunit alpha
RA NZ_CP022686 1,632,501 T→G 10.9% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,640,925 A→C 9.0% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,231 A→T 7.0% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 11.7% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 8.8% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 8.2% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,758,771 T→G 8.1% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,804,957 C→A 7.1% pseudogene (238/934 nt) CIW80_RS09020 → nucleotidyl transferase AbiEii/AbiGii toxin family protein
RA NZ_CP022686 1,832,023 T→G 10.6% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,833,750 A→T 11.4% V466D (GTC→GAC)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,843,277 A→C 6.4% F339V (TTT→GTT)  glcA ← glycolate permease GlcA
RA NZ_CP022686 1,860,077 G→T 12.1% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,888,373 C→A 13.6% R245R (CGA→AGA)  yqhG → YbjP/YqhG family protein
RA NZ_CP022686 1,911,602 T→G 7.9% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,954,946 A→C 11.3% V84G (GTC→GGC)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,974,549 T→G 5.1% V986G (GTC→GGC)  ebgA → beta‑galactosidase subunit alpha
RA NZ_CP022686 2,027,390 A→C 7.2% K80N (AAA→AAC agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,035,557 G→T 10.3% I126I (ATC→ATA rsmI ← 16S rRNA (cytidine(1402)‑2'‑O)‑methyltransferase
RA NZ_CP022686 2,049,089 T→G 20.8% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 14.6% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,076,554 T→G 13.3% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 11.8% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 5.7% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,150,071 T→G 12.8% G290G (GGT→GGG prmA → 50S ribosomal protein L11 methyltransferase
RA NZ_CP022686 2,159,639 G→T 7.2% D156Y (GAT→TAT)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,177 A→C 5.5% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,185,071 A→C 7.9% G47G (GGT→GGG rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,185,075 A→C 9.9% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,209,289 T→A 5.4% T187S (ACC→TCC)  chiA ← bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 6.9% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,257,157 A→C 6.1% S85A (TCA→GCA)  hofM ← DNA utilization protein HofM
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,324 A→C 15.5% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,489 T→G 11.3% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,362,607 T→G 8.6% L36R (CTT→CGT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,362,972 T→G 7.8% F158V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,380,936 A→C 8.6% K186N (AAA→AAC pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,386,496 T→G 9.8% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,393,013 T→G 6.1% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 18.8% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,318 G→T 8.8% Q105K (CAA→AAA)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,581 A→C 20.0% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 7.1% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,872 A→C 5.9% K761T (AAA→ACA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,558,864 T→G 5.1% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,572,721 A→C 15.7% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,703,299 A→C 10.2% intergenic (‑203/‑277) yieP ← / → CIW80_RS13540 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA NZ_CP022686 2,719,163 T→G 6.3% K123N (AAA→AAC ilvY ← HTH‑type transcriptional activator IlvY
RA NZ_CP022686 2,733,594 C→A 8.9% I60I (ATC→ATA rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,736,578 C→A 7.4% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 11.0% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 10.9% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,769,181 A→C 9.5% V52G (GTG→GGG)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,196 A→C 16.1% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,821,644 A→C 7.8% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,834,898 A→C 8.4% K601N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 16.7% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 10.0% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 10.3% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,874,073 A→C 8.2% T62P (ACC→CCC)  CIW80_RS14390 → carbohydrate porin
RA NZ_CP022686 2,900,823 G→T 5.1% E28* (GAA→TAA)  zapB → septal ring assembly protein ZapB
RA NZ_CP022686 2,901,370 A→C 5.7% V63G (GTC→GGC)  rraA ← ribonuclease E activity regulator RraA
RA NZ_CP022686 2,904,852 T→G 9.6% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,918 T→G 5.9% T217P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,981,612 A→C 8.9% T567P (ACC→CCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,981,625 A→C 7.1% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 9.6% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,017,622 A→C 13.9% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,090,101 A→C 9.6% V9G (GTT→GGT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,136,940 T→G 7.1% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,948 G→T 10.1% L158L (CTC→CTA phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,184,651 A→C 8.6% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,218,260 T→G 6.7% L233R (CTT→CGT)  miaA → tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
RA NZ_CP022686 3,265,084 T→G 17.3% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,297,360 T→G 10.1% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,342 A→T 8.1% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,345 G→T 8.4% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,357 T→G 6.5% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,363,064 C→A 6.2% intergenic (+763/‑28) CIW80_RS16815 → / → CIW80_RS16820 helix‑turn‑helix domain‑containing protein/Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 3,390,614 G→T 8.5% A143A (GCC→GCA sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 16.7% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,832 T→A 10.9% F274Y (TTC→TAC)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,451,843 G→C 14.4% E278Q (GAA→CAA)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,215 A→T 9.2% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,221 T→G 13.9% intergenic (‑30/+5) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 16.7% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 18.5% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 9.2% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,468,357 A→C 10.6% T300P (ACG→CCG)  yjjU → patatin family protein
RA NZ_CP022686 3,504,054 T→G 7.0% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,526,230 G→T 7.7% A209S (GCC→TCC)  dapB → 4‑hydroxy‑tetrahydrodipicolinate reductase
RA NZ_CP022686 3,634,614 A→C 12.5% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,652,079 T→G 8.1% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,090 T→G 8.0% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 11.8% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,731,466 T→C 51.8% intergenic (+20/‑35) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,470 C→T 53.3% intergenic (+24/‑31) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,868,846 T→G 12.1% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,871,944 C→T 84.6% E2K (GAA→AAA)  ecpR ← ECP biosynthesis operon DNA‑binding transcriptional regulator EcpR
RA NZ_CP022686 3,928,369 T→G 11.2% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 14.2% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,690 A→C 6.0% G319G (GGT→GGG frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,981,933 T→G 13.1% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,238 C→G 14.6% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,250 C→G 8.6% intergenic (+96/‑248) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,290 G→C 10.3% E106Q (GAA→CAA)  glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,041,543 A→T 6.7% I746I (ATT→ATA acrB ← efflux RND transporter permease AcrB
RA NZ_CP022686 4,056,962 C→A 13.8% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,067,840 A→C 5.8% K7N (AAA→AAC ybaQ → HigA family addiction module antitoxin
RA NZ_CP022686 4,214,435 G→C 9.0% intergenic (‑8/+27) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,290,599 T→G 7.3% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,571,439 T→G 9.5% H59P (CAC→CCC)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,583,770 G→T 12.3% D74Y (GAT→TAT)  CIW80_RS22900 → YfjI family protein
RA NZ_CP022686 4,678,041 A→C 19.3% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 27.6% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,712,763 A→C 18.1% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,712,985 A→C 7.0% intergenic (‑67/+67) yceB ← / ← grxB lipoprotein/glutaredoxin 2
RA NZ_CP022686 4,748,646 G→T 7.8% D380Y (GAT→TAT)  ptsG → PTS glucose transporter subunit IIBC
RA NZ_CP022686 4,762,516 T→G 7.2% K368N (AAA→AAC mfd ← transcription‑repair coupling factor
RA NZ_CP022686 4,764,877 T→G 16.0% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,896 C→G 7.8% intergenic (‑76/‑185) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,914 C→A 12.1% intergenic (‑94/‑167) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,809,441 T→G 9.2% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,839,687 T→G 7.5% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,178 T→G 16.0% F102V (TTC→GTC)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,905,221 T→G 12.4% L116R (CTT→CGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,916,128 A→C 7.9% intergenic (+255/+30) tdk → / ← CIW80_RS24885 thymidine kinase/IS4 family transposase
RA NZ_CP022686 5,001,164 A→C 17.0% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,042,151 T→G 9.3% V594V (GTT→GTG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,289 T→C 6.1% N640N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,304 T→C 7.1% N645N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,843 A→G 5.7% V1158V (GTA→GTG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 14.8% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 682–2618 3044–2957 340–2363 7876 [7691] [7573] 7732 JNG15_RS00010–mobA JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2771–2840 5510–3245 406–2740 7918 [7510] [6620] 7665 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 1522879 =NA (NA)12957 (2.220) 235/250 NT 92.6% intergenic (‑392/‑74) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pHH‑29_RBS 1429 = 1028 (0.090)intergenic (–/‑7) –/ompA –/–
* ? NZ_CP022686 = 1524130NA (NA)14103 (2.410) 240/250 NT 93.2% intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
?pHH‑29_RBS = 1429 1037 (0.090)intergenic (–/‑7) –/ompA –/–
* ? NZ_CP022686 = 4787823185 (0.860)36 (0.010) 22/246 NT 5.6% intergenic (‑76/‑20) CIW80_RS24075/CIW80_RS24080 IS3‑like element ISEc52 family transposase/super‑infection exclusion protein B
?pHH‑29_RBS 1431 = 1026 (0.090)intergenic (–/‑5) –/ompA –/–