breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 9.1% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 192,617 A→C 11.7% E676D (GAA→GAC rsxC → electron transport complex subunit RsxC
RA NZ_CP022686 238,312 G→T 17.5% Y89* (TAC→TAA sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 360,227 T→G 19.3% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,256 C→A 24.3% G25V (GGA→GTA)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 377,307 A→C 13.2% K147N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 402,874 A→C 10.5% pseudogene (785/2061 nt) ptrB ← oligopeptidase B
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 531,408 A→C 14.6% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 10.7% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 24.8% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 21.5% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 16.8% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,001 A→T 10.7% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,052 T→G 10.4% T23P (ACC→CCC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 21.3% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 22.7% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 16.2% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 881,733 A→C 12.1% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 17.6% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,027 A→T 12.4% intergenic (+96/‑161) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 18.9% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 972,697 A→C 7.3% T7P (ACG→CCG)  rcsD → phosphotransferase RcsD
RA NZ_CP022686 1,000,415 A→T 24.0% P950P (CCT→CCA yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,085 C→A 12.2% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,419 T→A 15.7% intergenic (‑268/‑5) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,174,272 A→C 16.7% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 14.3% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,239,833 G→T 15.2% intergenic (‑130/+38) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,269,551 T→G 12.0% K1124N (AAA→AAC CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,353,233 A→C 14.3% V72G (GTT→GGT)  yfiF ← tRNA/rRNA methyltransferase
RA NZ_CP022686 1,360,724 G→T 10.6% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,736 G→T 10.5% intergenic (‑71/+253) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 8.2% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,366,270 T→G 9.0% intergenic (‑271/+171) CIW80_RS06740 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA NZ_CP022686 1,479,607 A→C 9.6% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,258 G→T 15.4% S232I (AGT→ATT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,310 G→T 11.4% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 22.4% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,513,711 A→C 6.7% N93T (AAC→ACC)  barA → two‑component sensor histidine kinase BarA
RA NZ_CP022686 1,546,996 T→A 15.8% intergenic (+18/‑16) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,597,235 T→G 11.8% N19H (AAC→CAC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,597,265 G→T 7.7% Q9K (CAA→AAA)  thyA ← thymidylate synthase
RA NZ_CP022686 1,604,863 G→C 7.9% G378G (GGC→GGG lplT ← lysophospholipid transporter LplT
RA NZ_CP022686 1,632,501 T→G 11.6% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,868 C→A 24.7% E246* (GAA→TAA)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,633,870 G→T 13.5% A245E (GCA→GAA)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,674,224 T→G 15.8% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 12.2% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 12.8% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,145 A→C 9.1% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,734,237 A→G 100% W156R (TGG→CGG)  papX ← HTH‑type transcriptional regulator PapX
RA NZ_CP022686 1,758,771 T→G 8.0% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,949,882 G→C 7.9% G395A (GGA→GCA)  CIW80_RS09700 → RNA‑guided endonuclease TnpB family protein
RA NZ_CP022686 1,954,923 T→G 16.0% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,049,089 T→G 13.9% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,167 G→C 7.5% Q615E (CAG→GAG)  infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,076,556 C→G 9.4% intergenic (+26/‑207) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,465 T→G 12.8% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 9.4% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,115 T→G 15.1% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 18.4% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,179,877 C→G 11.3% K35N (AAG→AAC rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,075 A→C 12.1% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,215,206 C→A 5.7% A41S (GCT→TCT)  tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,226,139 A→C 8.6% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,336,298 A→C 6.3% V277G (GTC→GGC)  ugpE ← sn‑glycerol‑3‑phosphate ABC transporter permease UgpE
RA NZ_CP022686 2,340,264 A→C 5.9% V91G (GTC→GGC)  livF ← high‑affinity branched‑chain amino acid ABC transporter ATP‑binding protein LivF
RA NZ_CP022686 2,393,013 T→G 5.9% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,398,896 A→C 11.8% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,576 C→A 12.2% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,579 T→A 19.7% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,460,581 A→C 19.9% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 8.2% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,533,236 A→C 9.2% L301R (CTG→CGG)  tdh ← L‑threonine 3‑dehydrogenase
RA NZ_CP022686 2,546,394 A→C 13.0% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,559,398 T→G 5.7% N461T (AAC→ACC)  ligB ← NAD‑dependent DNA ligase LigB
RA NZ_CP022686 2,572,705 A→C 15.1% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 12.7% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,717,385 T→G 8.8% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,736,578 C→A 8.2% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,780,110 T→G 10.0% F677C (TTT→TGT)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,808,135 C→A 13.7% P444T (CCT→ACT)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,811,353 C→G 7.9% A17P (GCC→CCC)  rfaH ← transcription/translation regulatory transformer protein RfaH
RA NZ_CP022686 2,868,740 T→G 14.2% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 12.5% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,937,268 G→T 6.4% P199Q (CCA→CAA)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,989,627 T→G 13.3% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,086,834 A→C 9.6% F708V (TTT→GTT)  uvrA ← excinuclease ABC subunit UvrA
RA NZ_CP022686 3,184,651 A→C 8.6% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,209,121 T→G 7.2% T236P (ACC→CCC)  rsgA ← small ribosomal subunit biogenesis GTPase RsgA
RA NZ_CP022686 3,225,828 A→C 6.1% K288N (AAA→AAC rnr → ribonuclease R
RA NZ_CP022686 3,265,084 T→G 15.1% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,348,357 T→G 9.3% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,371 T→G 8.5% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,610 C→G 20.1% D145H (GAC→CAC)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 13.3% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,444,785 T→G 5.6% T503P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,222 C→G 13.8% intergenic (‑31/+4) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 15.2% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,504,054 T→G 7.7% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,652,079 T→G 10.4% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,731,470 C→T 100% intergenic (+24/‑31) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,734,367 T→G 22.1% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 13.7% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,524 T→G 6.9% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 15.6% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,236 G→T 6.9% R1576I (AGA→ATA)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,850,155 T→G 10.2% intergenic (+53/‑39) gpt → / → frsA xanthine phosphoribosyltransferase/esterase FrsA
RA NZ_CP022686 3,868,846 T→G 14.2% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,909,238 T→G 7.3% D280E (GAT→GAG pdeL → DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
RA NZ_CP022686 3,925,289 A→C 13.7% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,935,324 T→G 15.7% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,949,969 T→G 10.6% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 4,004,135 T→G 6.7% R29R (CGT→CGG yajQ → nucleotide binding protein YajQ
RA NZ_CP022686 4,014,238 C→G 13.5% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 14.2% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,061,214 A→C 9.7% L432R (CTG→CGG)  ybaL ← YbaL family putative K(+) efflux transporter
RA NZ_CP022686 4,079,363 A→C 12.1% A158A (GCT→GCG ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,280,095 T→G 9.2% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,461,768 C→A 5.8% intergenic (+122/‑48) aroA → / → ycaL 3‑phosphoshikimate 1‑carboxyvinyltransferase/metallopeptidase YcaL
RA NZ_CP022686 4,503,300 A→C 15.5% K364T (AAG→ACG)  rlmKL → bifunctional 23S rRNA (guanine(2069)‑N(7))‑methyltransferase RlmK/23S rRNA (guanine(2445)‑N(2))‑methyltransferase RlmL
RA NZ_CP022686 4,571,449 G→T 12.7% R56S (CGT→AGT)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,587,091 T→G 12.8% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,632,182 T→G 10.5% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,764,877 T→G 16.0% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,170 T→G 14.2% V99G (GTT→GGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,947,335 T→G 11.2% V42G (GTT→GGT)  rluB → 23S rRNA pseudouridine(2605) synthase RluB
RA NZ_CP022686 5,001,138 G→T 13.9% S154S (TCG→TCT ompG → monomeric porin OmpG
RA NZ_CP022686 5,042,250 A→T 9.5% V627V (GTA→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,304 T→G 13.5% N645K (AAT→AAG CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,484 T→G 6.8% intergenic (‑1688/‑47) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,762 G→T 14.1% E52* (GAA→TAA)  IL2wt no signal →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 303–2619 3084–2957 339–2782 6697 [6587] [6679] 7281 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4738–4809 5478–5073 265–741 3635 [3471] [0] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 4514374 =146 (0.860)4 (0.000)
+AGCT
3/242 NT 5.1% coding (59/1053 nt) ompA porin OmpA
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 7 (0.000)coding (1/399 nt) IL2wt no signal